ISfinder Mirror Site

ISFsp14

Family IS1380
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009921	ND	Frankia sp.	Frankia sp. EAN1pec

DNA section

IS Length : 1696 bp

Ends

IR Length : 9/15

IRL : CCTCCGGCTTTCACGGCGATGATCGTCTGTTGGGGTGTCGGAAATAGGAA
IRR : CCCAGATCTTTCGCGCCTTGGCCAGGTGCGATCGCGGGCTGGCCTCCCAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCTGGCCACGACGCCA	CTAG	CAGGACACGGGCCCGATCTC	4
ACCGGAGCCGACCAGA	CTAG	TGTAGATCACGTGACG	4
TTGCGATCTCTGGTCG	CTAG	GGTCTTCAGATTTGTG	4
CAGCCATGCGCGCATA	TTAG	CGGCCCGCAAAACGAC	4

DNA sequence

CCTCCGGCTTTCACGGCGATGATCGTCTGTTGGGGTGTCGGAAATAGGAAAGTGCTCCTGAACTGGGAGGATCGGGCTTGTCTAGGACCTGATCCGTTCC
AATCCAAGGAGCACCTTCATGGTGCAGAGTACCGGTGTTTACCCCTCTGTCCGCATCGACGCCGCCGGGCGGGGTGTCGTGTCGGCGGCGGGCAGCGTGC
TGCTGACCGAGACGGTCCGCGTCAGCGGGCTGGGTCGGTTGCTGTCCGACGCGCTCGCGCCGTGGCGGTCACCGCTGGCGACCCATGACCCGGCGAAGGT
ACTGACCGATCTGGCCGTGGCGCTGGCGTTGGGCGGGGACTGCCTGGCCGACATCGCCCAGCTACGCGCGGAGCCGGAGTTGTTCGGGCTGGTGGCCTCG
GACCCGACGGTGTCACGCACGATCGACCGGCTCGCCGGCGACGCCGAGAAGGTCCTGACGGCACTCGACCGGGCCCGCGCGGCAGCGCGGGGCCAGGTAT
GGGCGGCGGCCGGGGAACACGCCCCCGACCACACGGTGTCGGTGCACGACCCGCTGATCGTGGATCTCGACGCGACACTGGTCACCGCCCATTCCGAGAA
ACAAGACGCGGCGCCGACATTCAAACGCGGCTTCGGGTTCCACCCGTTGTGGGCGTTCATCGACCACGGCCAGGCCGGCACCGGGGAACCCGCGACGGTC
CTGCTGCGCCCCGGTAACGCCGGGTCGAACACCGCCGCCGACCACATCACCGTCGCCACCGGCGCCCTCGCCCAGCTCCCGGCCCGGCTACGCCGCTCCC
GCAAGGTACTGATCCGCGCCGACTCCGCCGGTGGGACCCACGCCTTCCTCGCCTGGGCGCACCAGCGCAGGCTGGCCTACTCGGTCGGGTTCACCCTGCC
CGACAACGCCGCCCAGCTGATCGGCGAGATCCCCGCGAAGGCGTGGACACCGGCCTACGACGCCGACCGCCAGCCCCGGCCCGGCGCGTTCGTCGCCGAA
CTGTCCGGGCTGATGGACCTGACCGGCTGGCCACCGGGCATGCGTGTCCTCGTCCGCAAGGAACGCCCGCACCCCGGCGCGCAGCTGCGTGTCACCGACG
TCGACGGCAACCGGATCACCGCGTTCGCGACCAACGCCGCCCGCGGGCAGCTCGCCGACCTCGAACTACGCCACCGCCGCCGGGCCCGCTGCGAGGACCG
TATCCGAACCGCCAAGGACACCGGTCTGACCAACCTGCCGCTGCACGACTTCACCCAGAACCAGATCTGGTGCGCCCTTGTCGCACTCGCTCTCGATCTG
ATCGCCTGGACCCAGATGCTCGCCCTCGCCGGCCAGCCCGCGCGACGCTGGGAACCGAAACGGCTACGCCACCGCCTGTTCTGGCTCGCAGGCCGGCTCG
CCCACCACGCCCGCCGCAGCACCCTGCACCTGCCCCCGCACCACCCCTGGGCCCCCCTCGCCCTCCAGGCGATCACCACACTGCGCGCACTGCCCGCCCC
CGGATAGCCCGCGACCACAGACCTCCCACCCCACCGACCAGAAGGAACCCCACCCGGACGTGGACCGGGCGCCCACCCCGCGCGACAGCCGGGGCACTCC
GCCACGGTCACACCCGGACCGAGCGGCAAAACTCAACATCAACAGAGTGGGAGGCCAGCCCGCGATCGCACCTGGCCAAGGCGCGAAAGATCTGGG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1389 bp	462 aa	119	1507	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVQSTGVYPSVRIDAAGRGVVSAAGSVLLTETVRVSGLGRLLSDALAPWRSPLATHDPAKVLTDLAVALALGGDCLADIAQLRAEPELFGLVASDPTVSR
TIDRLAGDAEKVLTALDRARAAARGQVWAAAGEHAPDHTVSVHDPLIVDLDATLVTAHSEKQDAAPTFKRGFGFHPLWAFIDHGQAGTGEPATVLLRPGN
AGSNTAADHITVATGALAQLPARLRRSRKVLIRADSAGGTHAFLAWAHQRRLAYSVGFTLPDNAAQLIGEIPAKAWTPAYDADRQPRPGAFVAELSGLMD
LTGWPPGMRVLVRKERPHPGAQLRVTDVDGNRITAFATNAARGQLADLELRHRRRARCEDRIRTAKDTGLTNLPLHDFTQNQIWCALVALALDLIAWTQM
LALAGQPARRWEPKRLRHRLFWLAGRLAHHARRSTLHLPPHHPWAPLALQAITTLRALPAPG

Blast result :

Comments

ISFsp14 is 73% aa similar to IS1676.

References

1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Saunders,E., Brettin,T., Bruce,D., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Rawnsley,T., Niemann,J., Benson,D.R., Huang,Y., Mastronunzio,J., Bassi,C.A., Tisa,L.S. and Richardson,P. (2007) Direct submission GenBank.