ISPa33
- Family IS1380
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF498413 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Pseudomonas mendocina LM7 Arthrobacter aurescens TC1 plasmid TC1 Pseudomonas sp. LM13 Arthrobacter aurescens TC1 plasmid pAA1 Pseudomonas sp. |
DNA section
IS Length : 1561 bp
Ends
IR Length : 16
IRL : CCCGGATTTCTCATGACCGCATAAACAAAAACGGCTGAAAGCCTTATGAT
IRR : CCCGGATTTCTCATGAGGCCGGCAGGCGTGGCCGGTGAAGCCTCGGTGTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGCACCGAA | CTAG | GTCTTGTCGA | 4 |
| CACGGCTGAG | TTAG | CAAACGGCGC | 4 |
| GATCTTGATC | CTAA | CGATGTAACC | 4 |
DNA sequence
CCCGGATTTCTCATGACCGCATAAACAAAAACGGCTGAAAGCCTTATGATTCTTGTCGCCAAACAAAGCCCCATAAGTCTTCAGCCGCGCACAAAATGCT
ACCAGAAAAACTCCACTTTTCACCCCTCTCTCGCCGCCTCGTCGAGGCCTCTTTCACCGGTGGTCACATCACCTCGGATGCGGGCTTGTTGCTGCTGCGC
GAGGTCGACCGGCAACATGGCTTGACCCGCCGCCTGGCCAAAATCGTTCCCGATCGGCGTGATCCTGGGCGTGTCCAGCATGGACTGCAGACACTGCTCG
CCCAGCGTATCTATGCACTGGCCGCCGGCTACGAGGATCTCAACGATCATGACGGCCTGCGCCATGACTACGCGCTGCAGACTGCGGTCAACCGCCTGCA
ACCGCTGGCCGGCAAATCCACTCTGGGGCGCCTGGAACAGCAAGCCGATCGCGAAACGGTGGTGCAAGCCCATCGCCTGCTGTGGGAGCACTTCATCGCC
CAGCATGACCAGGCGCCGGCTGAGATCGTGCTCGACTTCGATGCGACCGATGTGCCGGTGCATGGTGATCAGGAAGGGCGCTTCTTCCATGGCTATTACG
ACCATTACTGCTTCCTGCCGCTCTATGTGTTCTGTGGTCGCCATCTGCTGGTGAGCTATTTGCGCCCGAGCAACATCGATGGCGCCCGACACAGTTGGGC
CATCCTGGCACTGCTGGTCAAGTTCATTCGCCGCTTCTGGCCAGAGACCCGCATCGTGTTCCGCGGTGACGGCGGTTTTTGCCGACATCGCATGCTCGAC
TGGTGTGACCGCAAGCAGGTGGATTATGTGGTGGGTCTGGCCCGCAACACCCGCCTGCAGCGGATGGCGGCGCCGGCCATGCAAGCGGTCGCCGAGGCCT
ACCAAGAAACCGGGCAGAAGATGTCCGGCGTGTATCGCTTCCTCTATCAGGCCGGTAGTTGGCGTAGGCGCCGCCTGGTCGTGTCGCGCCTGGAACATGG
CGAGCAGGGTGCCAATCCACGCTTCATCGTCGTGTCCCGCTTTGCCGAGGATGCTGCCCAGCAGTACTACGAAGACTACTGCGGCCGTGGCGACATGGAG
AATCGGATCAAGGACCAACAGTTGGATCTGTTCGCCGACCGCACCTCCAGCCCACGCTGGTGGACCAACCAGTGGCGGCTGATGCTGTCGGCCTTTGCCT
ATGTGCTGTTCGAACGGCTACGCGATCATCTGCAGGGCACCGCATTGGCGCGCATGAGCATCCATAACCTGCGCTTGAAACTGCTCAAGATCGGTGCGGT
GATCATTCGCAACAGCCGGCGCATCCGCGTGCTGATCAGCGAGGCCTATCCGCATCAGGAACTCTTTGCGGGGCTGGTTGCCCGCCTCAAGCCGACCTGA
ACAGGCGGGCGCCCCCGGCCCGGCTCAATGGGGGAAAGGGGGCAGTGCGCCCCATACGCGGGAATTGAATAAAAAACGATCAATTTTTAGCGGCGGAAGG
CAGGTTGTTGATACACCGAGGCTTCACCGGCCACGCCTGCCGGCCTCATGAGAAATCCGGG
ACCAGAAAAACTCCACTTTTCACCCCTCTCTCGCCGCCTCGTCGAGGCCTCTTTCACCGGTGGTCACATCACCTCGGATGCGGGCTTGTTGCTGCTGCGC
GAGGTCGACCGGCAACATGGCTTGACCCGCCGCCTGGCCAAAATCGTTCCCGATCGGCGTGATCCTGGGCGTGTCCAGCATGGACTGCAGACACTGCTCG
CCCAGCGTATCTATGCACTGGCCGCCGGCTACGAGGATCTCAACGATCATGACGGCCTGCGCCATGACTACGCGCTGCAGACTGCGGTCAACCGCCTGCA
ACCGCTGGCCGGCAAATCCACTCTGGGGCGCCTGGAACAGCAAGCCGATCGCGAAACGGTGGTGCAAGCCCATCGCCTGCTGTGGGAGCACTTCATCGCC
CAGCATGACCAGGCGCCGGCTGAGATCGTGCTCGACTTCGATGCGACCGATGTGCCGGTGCATGGTGATCAGGAAGGGCGCTTCTTCCATGGCTATTACG
ACCATTACTGCTTCCTGCCGCTCTATGTGTTCTGTGGTCGCCATCTGCTGGTGAGCTATTTGCGCCCGAGCAACATCGATGGCGCCCGACACAGTTGGGC
CATCCTGGCACTGCTGGTCAAGTTCATTCGCCGCTTCTGGCCAGAGACCCGCATCGTGTTCCGCGGTGACGGCGGTTTTTGCCGACATCGCATGCTCGAC
TGGTGTGACCGCAAGCAGGTGGATTATGTGGTGGGTCTGGCCCGCAACACCCGCCTGCAGCGGATGGCGGCGCCGGCCATGCAAGCGGTCGCCGAGGCCT
ACCAAGAAACCGGGCAGAAGATGTCCGGCGTGTATCGCTTCCTCTATCAGGCCGGTAGTTGGCGTAGGCGCCGCCTGGTCGTGTCGCGCCTGGAACATGG
CGAGCAGGGTGCCAATCCACGCTTCATCGTCGTGTCCCGCTTTGCCGAGGATGCTGCCCAGCAGTACTACGAAGACTACTGCGGCCGTGGCGACATGGAG
AATCGGATCAAGGACCAACAGTTGGATCTGTTCGCCGACCGCACCTCCAGCCCACGCTGGTGGACCAACCAGTGGCGGCTGATGCTGTCGGCCTTTGCCT
ATGTGCTGTTCGAACGGCTACGCGATCATCTGCAGGGCACCGCATTGGCGCGCATGAGCATCCATAACCTGCGCTTGAAACTGCTCAAGATCGGTGCGGT
GATCATTCGCAACAGCCGGCGCATCCGCGTGCTGATCAGCGAGGCCTATCCGCATCAGGAACTCTTTGCGGGGCTGGTTGCCCGCCTCAAGCCGACCTGA
ACAGGCGGGCGCCCCCGGCCCGGCTCAATGGGGGAAAGGGGGCAGTGCGCCCCATACGCGGGAATTGAATAAAAAACGATCAATTTTTAGCGGCGGAAGG
CAGGTTGTTGATACACCGAGGCTTCACCGGCCACGCCTGCCGGCCTCATGAGAAATCCGGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1305 bp | 434 aa | 96 | 1400 | + | No |
Chemistry : DDE
ORF sequence :
MLPEKLHFSPLSRRLVEASFTGGHITSDAGLLLLREVDRQHGLTRRLAKIVPDRRDPGRVQHGLQTLLAQRIYALAAGYEDLNDHDGLRHDYALQTAVNR
LQPLAGKSTLGRLEQQADRETVVQAHRLLWEHFIAQHDQAPAEIVLDFDATDVPVHGDQEGRFFHGYYDHYCFLPLYVFCGRHLLVSYLRPSNIDGARHS
WAILALLVKFIRRFWPETRIVFRGDGGFCRHRMLDWCDRKQVDYVVGLARNTRLQRMAAPAMQAVAEAYQETGQKMSGVYRFLYQAGSWRRRRLVVSRLE
HGEQGANPRFIVVSRFAEDAAQQYYEDYCGRGDMENRIKDQQLDLFADRTSSPRWWTNQWRLMLSAFAYVLFERLRDHLQGTALARMSIHNLRLKLLKIG
AVIIRNSRRIRVLISEAYPHQELFAGLVARLKPT
LQPLAGKSTLGRLEQQADRETVVQAHRLLWEHFIAQHDQAPAEIVLDFDATDVPVHGDQEGRFFHGYYDHYCFLPLYVFCGRHLLVSYLRPSNIDGARHS
WAILALLVKFIRRFWPETRIVFRGDGGFCRHRMLDWCDRKQVDYVVGLARNTRLQRMAAPAMQAVAEAYQETGQKMSGVYRFLYQAGSWRRRRLVVSRLE
HGEQGANPRFIVVSRFAEDAAQQYYEDYCGRGDMENRIKDQQLDLFADRTSSPRWWTNQWRLMLSAFAYVLFERLRDHLQGTALARMSIHNLRLKLLKIG
AVIIRNSRRIRVLISEAYPHQELFAGLVARLKPT
Blast result :
Comments
ISPa33 is 64% aa similar to ISThsp13.
An isoform of ISPa33 has been identified in Pseudomonas mendocina strain LM7(KJ920393) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis, this isoform is present in 6 copies in the genome of Pseudomonas mendocina strain LM7 and in 4 copies in the genome of Pseudomonas sp. LM13. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas sp. LM8, Pseudomonas sp. LM11, Pseudomonas sp. LM12, Pseudomonas sp. LM14, Pseudomonas sp. ZM2.
File updated the January 22 2015 with the information provided by Szuplewska M.
An isoform of ISPa33 has been identified in Pseudomonas mendocina strain LM7(KJ920393) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis, this isoform is present in 6 copies in the genome of Pseudomonas mendocina strain LM7 and in 4 copies in the genome of Pseudomonas sp. LM13. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas sp. LM8, Pseudomonas sp. LM11, Pseudomonas sp. LM12, Pseudomonas sp. LM14, Pseudomonas sp. ZM2.
File updated the January 22 2015 with the information provided by Szuplewska M.
References
1] ISfinder annotation (2009)
2] Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V., Ivey,R.G., Zhou,Y., Kaul,R., Clendenning,J.B. and Olson,M.V. (2002) J. Bacteriol. 184 (13), 3614-3622
3] Szuplewska, M., and Bartosik, D. (2014) Direct submission.
2] Raymond,C.K., Sims,E.H., Kas,A., Spencer,D.H., Kutyavin,T.V., Ivey,R.G., Zhou,Y., Kaul,R., Clendenning,J.B. and Olson,M.V. (2002) J. Bacteriol. 184 (13), 3614-3622
3] Szuplewska, M., and Bartosik, D. (2014) Direct submission.