ISfinder Mirror Site

ISMaq4

Family IS1380
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008740	ND	Marinobacter aquaeolei	Marinobacter aquaeolei VT8

DNA section

IS Length : 1559 bp

Ends

IR Length : 17/18

IRL : CCCGGATTTCTCATGAGGGGATAAAAGAAAGCGGCTGAAAGTGTTAAAAT
IRR : CCCGGATTTCTCATAGGTCCCCGGCAAGTCTCGCAGACTTTGCGGGTTAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGATAGTCTG	CTAG	ACGCCTGTAT	4
CCTAAGAGGG	CTAG	TATTTCAGAT	4
AACCGGCAAG	CTAG	CATCAATGAG	4
TGGGGCAATA	TTAG	TACTCAGATC	4

DNA sequence

CCCGGATTTCTCATGAGGGGATAAAAGAAAGCGGCTGAAAGTGTTAAAATTTCAGGACCTTACACCATCAATCAACACCAAAAGCCGCTATCCAAAATGG
TACCTGAAAAACTGACCTTCTCGCCACTGTCTCGCCGTCAAATTGAAGCCGATTTCTCCGGCGGCCATATCACCTCCGATGCCGGCCTGCTCCTGCTTCG
CGAAGTCGACAATCAACACCAGCTCACTCAGCGCCTGGCTACGGTGCTGGACGATACCCGTAATCCGGTGCTGGTTCGCCACAAGCTTCAAACCATGATC
CGGCAACGGGTCTTTGGCGTGGCTGCCGGGTACGAAGATCTGAACGATCACGAAACGTTACGCGCTGACCAGGCACTTCAGACCGCGACCGGTGAAGAGG
CCATTCTGGCGGGCAAATCGACCTTGTGCCGCATGGAACAGCGCGTGGACCGGCAGGCGGTGGTCAAAGCCCACGAACTGCTGTGGCATCACTTCATTGA
ACAGCACGAGACTCCACCCAAGGAAATTGTGCTGGATTTTGATGGCACCGATGTTCCGGTGCATGGCGACCAGCCCGGCAAGTTCTTTAACGCCTACTAC
GATCACCATTGTTACTTCCCGCTGTACGTGTTCTGCGGTCGCCATCTGCTGGTGAGCTACCTGCGCACCAGCAACCGAAGCGACAGCCGCCACAGCTGGG
CCATTCTGGCCCTGCTGGTGAAGTTCATCCGGCAGTACTGGCCGGATACCCGCATCGTGTTCCGGGGTGACAGCGGCTTCTATCGCCCCCGATTGCTGAG
CTGGTGCGACCGGAATAACGTGGATTACCTTGTGGGCATCAGCAAAAACAGCCGGCTGCTCAAGGAGGTGGACGTACCCTCGATGCTGGTTCGCAGGGCT
CACGGGGAGTTGGGAGAAAAAGTCTCCGCCACCTACCGGTTCCAGTACCAGGCCCGAACCTGGAAACACCCCCGCTGGGTGATCGCGCGTCTGGAGGAGG
GCGAACTGGGTGCCAACCCCAGGTTCATTGTCAGCTCCCGTTACGACGACGGCGTTAAACTCTACTACGAGCAATACTGTGCCCGGGGTGACATGGAAAA
CCGGATCAAGGATCAGCAGTTGTGTCTGTTCGCAGATCGCACCTCCAGCACGCGGTGGTGGACCAATCAGTGGCGGTTGCTCCTGTCGGGCTTTGCCTAC
ACGTTGTTCGAGCGATTACGGGCCCACTTGAAGAAGACGCCCTTCGAGAGAATGAGCGCCAGTAACCTGCGGCTGAAGCTGATCAAGGTGGGCGCGGTCA
TCATTCGCAACACTCGGCGCATACGGGTGTTGATGAGTGATGCCTATCCTTACAAAGACGAACTCTCTGACCTGGTGCGTCGACTGGTTCCGGGATAGAA
GCCTGGGCTCCCCCGGCCCGACTCAATGGGGGAAAGGGGGCCGTGTGTCCGGACAACAGAAATTGATCAGTTATTAATCAATCGCGGCCGCAAATGAGCG
GTCATGGCGATAACCCGCAAAGTCTGCGAGACTTGCCGGGGACCTATGAGAAATCCGGG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1302 bp	433 aa	97	1398	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVPEKLTFSPLSRRQIEADFSGGHITSDAGLLLLREVDNQHQLTQRLATVLDDTRNPVLVRHKLQTMIRQRVFGVAAGYEDLNDHETLRADQALQTATGE
EAILAGKSTLCRMEQRVDRQAVVKAHELLWHHFIEQHETPPKEIVLDFDGTDVPVHGDQPGKFFNAYYDHHCYFPLYVFCGRHLLVSYLRTSNRSDSRHS
WAILALLVKFIRQYWPDTRIVFRGDSGFYRPRLLSWCDRNNVDYLVGISKNSRLLKEVDVPSMLVRRAHGELGEKVSATYRFQYQARTWKHPRWVIARLE
EGELGANPRFIVSSRYDDGVKLYYEQYCARGDMENRIKDQQLCLFADRTSSTRWWTNQWRLLLSGFAYTLFERLRAHLKKTPFERMSASNLRLKLIKVGA
VIIRNTRRIRVLMSDAYPYKDELSDLVRRLVPG

Blast result :

Comments

ISMaq4 is 61% aa similar to ISThsp13.

References

1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Edwards,K. and Richardson,P. (2007) Direct submission GenBank.