ISfinder Mirror Site

ISCysp9

Family IS1380
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_011729	ND	Cyanothece sp.	Cyanothece sp. PCC 7424

DNA section

IS Length : 2001 bp

Ends

IR Length : 17

IRL : CCCGGATTTCTCACAAAAAGTGTAATCGCACCCAACTGAACACCTTATCG
IRR : CCCGGATTTCTCACAAACTGACTGGATCAACCGATGGAAAGGGTGAAAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAGAAATGAG	GAAT	ATATTAAACT	4

DNA sequence

CCCGGATTTCTCACAAAAAGTGTAATCGCACCCAACTGAACACCTTATCGAACCTTAATTTGAACGAGAAAATTCTCGATCAGGACTTACCGGCAGTATG
CTAACTTTTATCAGCTTAATAACACTATCAATTAGAGTTTTGGGAAAATCCTATTTAATTAGCGTAATTTTTACGCTAATTAATAATTAATTATTTATTT
TTTGTCAAAAACAATAGCAATAAAGTTATAATTAGAATTAGATTAGGAAAAAATTAAAAAAATCTCTTTTTTATGGCTTTAATACCTTCAGCTAAAAACG
ACGAAAAACTCAATTTGGGAAAAATAAAAGGAAAAGAGGTGCTTGTCAATTTTGACGGAGGAAGAATAACCTCAAATGGAGGAATTGTTTTAATAGCCGA
GTTGGATAAAAAACTAAAAATAACACATAGATTTGCTAACTGCTTTCAAGATTATCGAAATTTATCTTATATAAATTACTCGGTTCATCAATTATTAGCG
CAAAGAATTTATGGAATAATTTTAGGTTATGAAGATATTAATGATCATGATAAACTACGTTATGACCCAGCATTGGCAATAGCCTTAGAAAAACTACATA
TTTTTGAGTCAAATGAAGAAAGATTAGCCGGAAAAAGCACAATTAACCGACTAGAATATTGTCCAGCTATAATTTTAAACCAAGAAGAGAGTCGCTATCA
TCGTATTGAACATCAACCAAGAGATATAGAAAAAGCTTTTATTGATATTTTTTTAGAATCTTATAAAAAGCCACCTCGACAAATTATTTTAGATATGGAT
GTCACGGACGACCCAGTGCATGGAAATCAAGAAAAAGCTTTCTTTAATAGTTATTATAAAGGAGTTTGTTATGCCCCTTTGTATATTTTCTGTGGGCATC
ATTTATTAGTGGCTAAACTTAGGCCTTCAAATGTAGATCCGGCTGATGGGGCATTAGAAGAATTACAACGAATTATAAAAATGATTAGAGAGAAATGGTC
TAATACTCATATATTAGTTAGAGGAGATAGTGCCTACGCTCGTGAAGAAATAATGAAATATTGCGAAGAACAAGACAATATTGATTATGTTCTGGCTATG
GCAACTAATAGCCAATTACAATTAAGAGCGGGTTCAGAAATAGAAAAAGCTAAGAATGATTATGAGAAAAAATTAGAGCCTGTCATAGAATTAATAGACA
GTCTTTTTAATAAAAATTTCGACTTGGAGGAAGTAAAAGAATTAGTTCCCGATTCCGTTTGGTATCGTTCAATATGTTATAAAACCGAGAAATCATGGAG
TCGTCAAAGAAGAGTTGTTACTAAAGTTTGTTATGGAAGTGATGGCTTAAAAATACGTCATATTGTAACATCTTTACCGGCTTGCAAAATCCCACCTTCA
AAACTTTATACTAAAAAATATTGTCCAAGAGGAGAAATGGAAAATCGAATTAAAGAGCAACAATTAGATTTGTTTGCCGACCGAACTTCAACACAAACAT
TTGAGAGTAACCAATTAAGGCTATGGTTATCTTCAATAGCTTATGTTTTAATGCAAGCTTTTCGTAAAATTTGTTTATCTAAAACCTCTTTATCCAAAGC
TACCGTTGGAACAATTCGTCTTAATTTATTGAAATTGGGAGCGCGAATAACTGTTACTTATAGAAGAATCATTATTGCTATAGCTAATTCTTGTCCTACT
CAACATCTTTTAGCTATTGCTAATTCTAGAATTCAAGCGATGGGAGATACAGGTTAAACTTTCTTTTTTAAATTATCACAAAAAAAATCAATAATTTCTG
TAACAGACTGAGAATATTTTCAGTTGATTTTGCCATCGAAACTTTAATAATAAATCCTAAATTCTGTCGAAAAATAAAAATCTATATTAATGATTTTAAT
GAATGCTGAATTGGATGACTTCCACAGCTTATCCAATTTTTTTTAAGCTCAATTTTCACCCTTTCCATCGGTTGATCCAGTCAGTTTGTGAGAAATCCGG
G

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1485 bp	494 aa	273	1757	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MALIPSAKNDEKLNLGKIKGKEVLVNFDGGRITSNGGIVLIAELDKKLKITHRFANCFQDYRNLSYINYSVHQLLAQRIYGIILGYEDINDHDKLRYDPA
LAIALEKLHIFESNEERLAGKSTINRLEYCPAIILNQEESRYHRIEHQPRDIEKAFIDIFLESYKKPPRQIILDMDVTDDPVHGNQEKAFFNSYYKGVCY
APLYIFCGHHLLVAKLRPSNVDPADGALEELQRIIKMIREKWSNTHILVRGDSAYAREEIMKYCEEQDNIDYVLAMATNSQLQLRAGSEIEKAKNDYEKK
LEPVIELIDSLFNKNFDLEEVKELVPDSVWYRSICYKTEKSWSRQRRVVTKVCYGSDGLKIRHIVTSLPACKIPPSKLYTKKYCPRGEMENRIKEQQLDL
FADRTSTQTFESNQLRLWLSSIAYVLMQAFRKICLSKTSLSKATVGTIRLNLLKLGARITVTYRRIIIAIANSCPTQHLLAIANSRIQAMGDTG

Blast result :

Comments

ISCysp9 is 61% aa similar to IS1380.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2008) Direct submission GenBank.