ISOih1

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
BA000028 ND Oceanobacillus iheyensis
Oceanobacillus iheyensis HTE831
DNA section
IS Length : 1563 bp

Ends


IR Length : 12/15

IRL : CAGGCTGTCGAGAAACCCTCGGCAGTCTATTTTTATGTGGGAAACAGCCC
IRR : CAGCTTGTAGAGAAAGTATGGTATTTTCTCTGCAAGCTTTTTTATATGGT

Insertion site


Left flankDirect repeatRight flankDR Length
GTTAGCACGTAAAG/TAGCCTCCTTTTTGGGAGGCTCTATTTTTAGTG24

DNA sequence

CAGGCTGTCGAGAAACCCTCGGCAGTCTATTTTTATGTGGGAAACAGCCCCAATTGACCGTAATTATTTGGTATAATAGAAGTACCAAACAAAGGCGGTG
ATGAGCTTGTTTCACACGAGAAAACACATGCAAAATGAAATGGAATTTGTTTGTTTAGAAGATTTAGTCCCCCAAGACCATTTACTACGGAAAATAGATA
AATACATTGATTTCTCTTTTATTATTGAAAAAGTAAAACCTTATTATTGTGAAGATAATGGTCGTCCCTCTCTAGACCCCCTTATTTTATTCAAAATGAT
GTTTGTTGGGTATTTTTTTGGCATTCGTTCGGAACGCCAACTTGAAAAGGAAATTCAAACAAATAATGCTTACCGCTGGTTTCTAGGTTTAAAACTGACA
GATCCAGTTCCTCACCATTCTACCATCAGTTGGAACCGTCGAACCCGTTTTAAAGATACGGAGATTTTCCAAGAAATTTTTGATGAAATTGTTCTCCAAG
CTATGAATCACCGTATGGTTGGAGGAAGAGTTCTTTTTACGGATTCCACTCATTTGAAAGCGAATGCCAATAAACATAAATTTAGTCGGGAAGAAGTCGA
AGTGGAAACACGAGACTACGTAGAAGAACTAAACCAAGCCATTCAAGAGGATCGTAAACAGAATGGAAAAAAAGGTCCTAAGAGATAGAGAGGAAGTGAA
ACAAGTCAAAACCATTCGGAAAAGTAAAACAGATCCGGAAAGTGGTTTTATGTCTCGGGACCACAAACAAGAAATGTTCTGTTACTTAGATCATCGAACC
ACAGATATGAAATATAATATTATTACAGACGCGTACGTCACTCCGGGAAATGTACATGATTCGGTCCCTTATCTGAAACGGTTAGACCGGCAAATCAAAC
GGTTTGATTTTATCGTGGAAGCAGTGGCTTTAGACTCCGGTTATTTAACGAATCCAATCTGTAAGGGATTAGCTGATCGGAATATATTCGGCGTCATCGC
TCACCGTCGCTATCATCCAACGAAAGGGTTATTTCCGAAGTGGAAATTCCGTTATGATGAAGACCGAGATAGTTATACTTGTCCGAATGGAGAAGAATTG
CCATATAAAACAACGACACGAGAAGGTTACAGAGAATTTAAATCAGACCCAAAAAAGTGTATATCCTGTCCGCTTTTGAAACAGTGTACCCGCTCGAATA
ATCATCAGAAAGTAGTCACCCGTCATGTTTGGGAAGAACATAAAGAACACGTGCGATTAAACCGGTTATCCGTTTCCGGAAAAAAGCTATACAAATTTAG
AAAAGAAAAAGTAGAGCGAAGTTTTGCAGACTCTAAAGAGCTGCATGGACTTCGCTACTGCAGGTTGAGGGGATTGCAGAATGCGGGAGAGCAAGTATTA
CTCACCGCAGCCTGCCAGAACATGAAGAAGATTGCCATCTACCTATCCAAGCAAGGATAGGTAGATGGGAGTTTATTTTCATGATATATCTTATTTTTCT
CTTGATTATTTTAACCATATAAAAAAGCTTGCAGAGAAAATACCATACTTTCTCTACAAGCTG
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
588 bp195 aa101688+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSLFHTRKHMQNEMEFVCLEDLVPQDHLLRKIDKYIDFSFIIEKVKPYYCEDNGRPSLDPLILFKMMFVGYFFGIRSERQLEKEIQTNNAYRWFLGLKLT
DPVPHHSTISWNRRTRFKDTEIFQEIFDEIVLQAMNHRMVGGRVLFTDSTHLKANANKHKFSREEVEVETRDYVEELNQAIQEDRKQNGKKGPKR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
828 bp275 aa6331460+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TKPFKRIVNRMEKKVLRDREEVKQVKTIRKSKTDPESGFMSRDHKQEMFCYLDHRTTDMKYNIITDAYVTPGNVHDSVPYLKRLDRQIKRFDFIVEAVAL
DSGYLTNPICKGLADRNIFGVIAHRRYHPTKGLFPKWKFRYDEDRDSYTCPNGEELPYKTTTREGYREFKSDPKKCISCPLLKQCTRSNNHQKVVTRHVW
EEHKEHVRLNRLSVSGKKLYKFRKEKVERSFADSKELHGLRYCRLRGLQNAGEQVLLTAACQNMKKIAIYLSKQG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1360 bp452 aa1011460+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSLFHTRKHMQNEMEFVCLEDLVPQDHLLRKIDKYIDFSFIIEKVKPYYCEDNGRPSLDPLILFKMMFVGYFFGIRSERQLEKEIQTNNAYRWFLGLKLT
DPVPHHSTISWNRRTRFKDTEIFQEIFDEIVLQAMNHRMVGGRVLFTDSTHLKANANKHKFSREEVEVETRDYVEELNQAIQEDRKQNGKKVLRDREEVK
QVKTIRKSKTDPESGFMSRDHKQEMFCYLDHRTTDMKYNIITDAYVTPGNVHDSVPYLKRLDRQIKRFDFIVEAVALDSGYLTNPICKGLADRNIFGVIA
HRRYHPTKGLFPKWKFRYDEDRDSYTCPNGEELPYKTTTREGYREFKSDPKKCISCPLLKQCTRSNNHQKVVTRHVWEEHKEHVRLNRLSVSGKKLYKFR
KEKVERSFADSKELHGLRYCRLRGLQNAGEQVLLTAACQNMKKIAIYLSKQG

 

Blast result :
Comments
ISOih1 is 58% aa similar to ISSag8. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2005)
2] Lu,J., Nogi,Y. and Takami,H.(2001) FEMS Microbiol. Lett. 205 (2), 291-297