ISPsp5
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U66917 | ND | Pseudomonas sp. | Pseudomonas sp. plasmid pADP-1 |
DNA section
IS Length : 1617 bp
Ends
IR Length : 21/25
IRL : GGGTCAATTCTGTTGAAAAAGTAGCGACCTCACCATGCCGTTGGCAAAAT
IRR : CGACCGATTCTGTTGAAAAAGTAGCTTCAGCGGCATCCTGCCGATCAGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCGGAGTGCCACCGTCCGCTTC | GAAAGCGGTCTCAACTGAAGGA | 0 |
DNA sequence
GGGTCAATTCTGTTGAAAAAGTAGCGACCTCACCATGCCGTTGGCAAAATTGCTCTGTCAGCGAGCGTGGGGGCGAACAGCATGATGGGACAGTTACCGG
GAGGACAACAGCGCCTGTTCTACTCGTTCAATCTGGAAGATCACGTCCCGGCCCAACACCTCCTGCGCAGCATCGATCACTGCTTGGATCTCAGTGACCT
GCGCGCCTACCTGGCCGATTTCTATAGCCCCATCGGACGCCCCTCGATTGACCCGGAATTGATGGTGCGCATGCTGGTCGTCGGCTACTGCTATGGCATT
CGTTCCGAGCGGCGATTGTGCGAAGAGGTACACCTGAATCTGGCCTATCGCTGGTTCTGCCGGCTGGGTCTTGAAGACGAAGTACCCAACCACTCAACCT
TCTCGAAGAATCGCCATGGACGTTTTCGTGACAGCGATCTATTCCGCTGGTTGTTCAATGAGGTGCTGCGGCGTTGCATGGCGGCCGGCCTGGTAAAGGG
TGAAGGTTTCGCCGTCGACGCCAGCATCATCAAGGCTGATGCCAGCCGGCAACGTGGGGTGGCGGGTGATGAGGTCGATTGGAGCGATCCAAAGTTCAGC
AGCCGCGCCGTGCGCGAGTACCTCAAAGCCCTCGATGAAGAGGCGCTGGCTGAAGCTCTCCCCAAGAAAATTTCGCTCACTGATCCTCAGTCCCGTTGGA
CAGCAGCGCCAGGCGGCCCGGCCTACTTTGCCTACTCCACGAACTACCTGATTGACACTGAGCATGGCGTGATCATGGATGTGGAAGCTACCCCGGCGCA
CCGTACTGCCGAAGTCGGTTCAACCAGGACGATGGTCGAGCGTGTCGAGGCGCAGTTCGCCCTCACGCCGGAACGCCTCATTGGCGACACCGCCTATGGC
ACTGCCCCGATGCTGGCCTGGATGGTTGAAGAGAAGAACATCGAGCCGCACGTGCCGGTGTGGGACAAGACTGAGCGCAAGGACGACAGCCTCTCCAGTA
ACGACTTCCACTGGAATCAGGAAGCCAATGAATATCGCTGCCCAGCCGGCAAACCACTACGCAGTGAATGGCGCGCCTTCACCCAGAAAAGATCGCGGGT
GACCAAGGCCAACACCATCATCTACCGCTCCAGCCAAACCGACTGCGCCACCTGTCCGCTGAAAGAAAAGTGCTGCCCCAACACGCCGAACCGGAAGATC
GTCCGCAGCATCCATGAGGCTGCCCGCGACGTGGCTCGACGCATCGCCAAGACGCAGGAATACCTAGTCTCTCGTTGCGAACGGAAGAAGGTGGAGATGC
TTTTCGCCCACCTCAAACGGATCATGAAACTCGACCGTTTACGACTGCGTGGCCTCATGGGTGCAACTGACGAATTCACCTTGGCCGCGACAGTGCAAAA
CCTACGCCGCATGGCCAAGCTTTTGCCTCAAGGGCCACCAATCACGGGATAGGTACGCCCGCTGCGAGCAGAAACCCTCAAATTAACCCTAAAACCTGAG
CAGGGACGCTCAGTGAAACGCCGAAAGGCAACTCGAAGTGGCTTGCAGCCACTTCGACAGCAGGCACACCTGATCGGCAGGATGCCGCTGAAGCTACTTT
TTCAACAGAATCGGTCG
GAGGACAACAGCGCCTGTTCTACTCGTTCAATCTGGAAGATCACGTCCCGGCCCAACACCTCCTGCGCAGCATCGATCACTGCTTGGATCTCAGTGACCT
GCGCGCCTACCTGGCCGATTTCTATAGCCCCATCGGACGCCCCTCGATTGACCCGGAATTGATGGTGCGCATGCTGGTCGTCGGCTACTGCTATGGCATT
CGTTCCGAGCGGCGATTGTGCGAAGAGGTACACCTGAATCTGGCCTATCGCTGGTTCTGCCGGCTGGGTCTTGAAGACGAAGTACCCAACCACTCAACCT
TCTCGAAGAATCGCCATGGACGTTTTCGTGACAGCGATCTATTCCGCTGGTTGTTCAATGAGGTGCTGCGGCGTTGCATGGCGGCCGGCCTGGTAAAGGG
TGAAGGTTTCGCCGTCGACGCCAGCATCATCAAGGCTGATGCCAGCCGGCAACGTGGGGTGGCGGGTGATGAGGTCGATTGGAGCGATCCAAAGTTCAGC
AGCCGCGCCGTGCGCGAGTACCTCAAAGCCCTCGATGAAGAGGCGCTGGCTGAAGCTCTCCCCAAGAAAATTTCGCTCACTGATCCTCAGTCCCGTTGGA
CAGCAGCGCCAGGCGGCCCGGCCTACTTTGCCTACTCCACGAACTACCTGATTGACACTGAGCATGGCGTGATCATGGATGTGGAAGCTACCCCGGCGCA
CCGTACTGCCGAAGTCGGTTCAACCAGGACGATGGTCGAGCGTGTCGAGGCGCAGTTCGCCCTCACGCCGGAACGCCTCATTGGCGACACCGCCTATGGC
ACTGCCCCGATGCTGGCCTGGATGGTTGAAGAGAAGAACATCGAGCCGCACGTGCCGGTGTGGGACAAGACTGAGCGCAAGGACGACAGCCTCTCCAGTA
ACGACTTCCACTGGAATCAGGAAGCCAATGAATATCGCTGCCCAGCCGGCAAACCACTACGCAGTGAATGGCGCGCCTTCACCCAGAAAAGATCGCGGGT
GACCAAGGCCAACACCATCATCTACCGCTCCAGCCAAACCGACTGCGCCACCTGTCCGCTGAAAGAAAAGTGCTGCCCCAACACGCCGAACCGGAAGATC
GTCCGCAGCATCCATGAGGCTGCCCGCGACGTGGCTCGACGCATCGCCAAGACGCAGGAATACCTAGTCTCTCGTTGCGAACGGAAGAAGGTGGAGATGC
TTTTCGCCCACCTCAAACGGATCATGAAACTCGACCGTTTACGACTGCGTGGCCTCATGGGTGCAACTGACGAATTCACCTTGGCCGCGACAGTGCAAAA
CCTACGCCGCATGGCCAAGCTTTTGCCTCAAGGGCCACCAATCACGGGATAGGTACGCCCGCTGCGAGCAGAAACCCTCAAATTAACCCTAAAACCTGAG
CAGGGACGCTCAGTGAAACGCCGAAAGGCAACTCGAAGTGGCTTGCAGCCACTTCGACAGCAGGCACACCTGATCGGCAGGATGCCGCTGAAGCTACTTT
TTCAACAGAATCGGTCG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1371 bp | 456 aa | 82 | 1452 | + | No |
Chemistry : DDE
ORF sequence :
MMGQLPGGQQRLFYSFNLEDHVPAQHLLRSIDHCLDLSDLRAYLADFYSPIGRPSIDPELMVRMLVVGYCYGIRSERRLCEEVHLNLAYRWFCRLGLEDE
VPNHSTFSKNRHGRFRDSDLFRWLFNEVLRRCMAAGLVKGEGFAVDASIIKADASRQRGVAGDEVDWSDPKFSSRAVREYLKALDEEALAEALPKKISLT
DPQSRWTAAPGGPAYFAYSTNYLIDTEHGVIMDVEATPAHRTAEVGSTRTMVERVEAQFALTPERLIGDTAYGTAPMLAWMVEEKNIEPHVPVWDKTERK
DDSLSSNDFHWNQEANEYRCPAGKPLRSEWRAFTQKRSRVTKANTIIYRSSQTDCATCPLKEKCCPNTPNRKIVRSIHEAARDVARRIAKTQEYLVSRCE
RKKVEMLFAHLKRIMKLDRLRLRGLMGATDEFTLAATVQNLRRMAKLLPQGPPITG
VPNHSTFSKNRHGRFRDSDLFRWLFNEVLRRCMAAGLVKGEGFAVDASIIKADASRQRGVAGDEVDWSDPKFSSRAVREYLKALDEEALAEALPKKISLT
DPQSRWTAAPGGPAYFAYSTNYLIDTEHGVIMDVEATPAHRTAEVGSTRTMVERVEAQFALTPERLIGDTAYGTAPMLAWMVEEKNIEPHVPVWDKTERK
DDSLSSNDFHWNQEANEYRCPAGKPLRSEWRAFTQKRSRVTKANTIIYRSSQTDCATCPLKEKCCPNTPNRKIVRSIHEAARDVARRIAKTQEYLVSRCE
RKKVEMLFAHLKRIMKLDRLRLRGLMGATDEFTLAATVQNLRRMAKLLPQGPPITG
Blast result :
Comments
ISPsp5 is 72% aa similar to ISShsp2.
References
1] ISfinder annotation (2008)
2] de Souza,M.L., Wackett,L.P., Boundy-Mills,K.L., Mandelbaum,R.T. and Sadowsky,M.J. (1995) Appl. Environ. Microbiol. 61 (9), 3373-3378;
3] Boundy-Mills,K.L., de Souza,M.L., Mandelbaum,R.T., Wackett,L.P. and Sadowsky,M.J.(1997) Appl. Environ. Microbiol. 63 (3), 916-923.
4] Sadowsky,M.J., Tong,Z., de Souza,M. and Wackett,L.P. (1998) J. Bacteriol. 180 (1), 152-158.
2] de Souza,M.L., Wackett,L.P., Boundy-Mills,K.L., Mandelbaum,R.T. and Sadowsky,M.J. (1995) Appl. Environ. Microbiol. 61 (9), 3373-3378;
3] Boundy-Mills,K.L., de Souza,M.L., Mandelbaum,R.T., Wackett,L.P. and Sadowsky,M.J.(1997) Appl. Environ. Microbiol. 63 (3), 916-923.
4] Sadowsky,M.J., Tong,Z., de Souza,M. and Wackett,L.P. (1998) J. Bacteriol. 180 (1), 152-158.