ISfinder Mirror Site

ISLac1

Family IS1182
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006814	ND	Lactobacillus acidophilus	Lactobacillus acidophilus NCFM

DNA section

IS Length : 1833 bp

Ends

IR Length : 37/41

IRL : GCAAAAAGTGTCTGGGAAATAACTAATCCTCGGATATGAAAAAAGAACGA
IRR : GAAAAGTGTCTGGGAAATAACTAATCCTCAGATACCAAAAGACGATCAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TAAAATTTGA	TAT	TTACCTCTTGT	3

DNA sequence

GCAAAAAGTGTCTGGGAAATAACTAATCCTCGGATATGAAAAAAGAACGATGATCTCCTAATGGATTTCATCGTTCTTTTTCTATACTTTAAGTGCCTTC
CTTGATATTATTGAATCACCACAAAACAAAAATGAAAGAAGGCAATCTTAATGTATCAAAATTATATCACAGGTCAAACTGCTTTAACACTTAATCTGGA
CTTTACTATTCCTAGCAATCATTTAGCTAATATCATCAGCCAGTTTGTGGATTCCATTCCTGAAGATGTCTTGCTAGGAAAAACTGCTAAAACAGGCCGC
CCTGCTTATCATCCAGCCATGATGCTTAAAATTTTACTTTTCGCATATTCTAGAAGAGTATTTTCAGGCAGAAAAATTGAATTAATGCTGGAAGAAAATG
TGCCTATGATGGTACTAGCAGACCATCAAAAGATTTCATACCATACTATCAATAACTTTAGATCTAGCAATCACGCCAATGAGTTGGTTAAGAAAAGCTT
TCTTTATTTTACGAATCTTTTAGAAGCAGAAGGTCTAATCAATGAAGGTGCTGTCTTTATCGATGGTACTAAGATCGAAGCCGATGCCAATCGCTATACT
TTCGTTTGGCGCAAAGCAGTTGAAAAGTTTCATAAAAAGCTAAAAGGCCAAGCAGTTGAGCTTTATGATGAACTGATCGCCAAGGAAGTAGTCAAAGCAA
TGGCTCAAGAGAAAGTCCGGACTAGTCAAGGCTTGGCAGAACTAGCACAAGATACAGAAGCAGAAATTGAAAAGCTGACTAAAAAAATTGAACAAGAGCC
CAGGGCTATTCCTGGTGGTTCACCTAGAAAAGCAAGAAGACGCAGGCTTAAAAAGCTGCTTCATAAACTGAGGAAAGACTATGTTCCTAGAATGAAGAAA
TACGAAGAAGCAGAAGAAATCTTTGCTGGCCGCAATAGTTATTCCAAGACCGACCACGATGCGACTTTCATGCATATGAAAGAAGATCATATGAAGAACG
GTCAGCTTAAGCCAGGCTACAATATTCAAGCAGCTACTACAAATCAGTATGTAGTTGACTTTGCTTTATATCATAATCCTACTGATTTCAGAACTTTAGA
ACCATTTTTAAAGCAGATGTCCACGCTGGATAAGTTTGATAAGATTGTCGCCGATGCTGGTTATGGCAGCGAGGACAATTATTCTATGCTAGAAAAAGAA
CATCCAGACAAAAAATACTACATTCCCTACACCATGTATGAAAAGGAAAAGACCAGAAAATATCGTAGTGATCCAACTAGGCTGGCTAACTGGTTCTATG
ATGAAAAGGATGATTACTATCTAGATCAAAATGGAGTCCGATTCAATTTCAAATATTACAGCCGGCGCAAAGATCGCTCGACTGACCAGGTTCGTGATTT
TAAGGTTTATGAAGCAGATGAGTTTCAACTGACGCCAGAATTAGAGCGTTTAGCTAAAACCAAGAGCGGCCGACAACGTCAAGTACGCTACAATCCCAAC
TGGCAGTATTTAAAAGAAAAAGCTAAAGAAGTTCTCCAAAGTCCTGAGGGCAGACACATCTATAGTATGAGAAAGTATGATGTGGAGCCAATTTTTGGAC
ATTTGAAGAACATCTTTGGCATACGCCGAACTCATTTAAGAGGCAAAAAAGAAGGTTGAAACGGATGTTGGAATAGCCTTCATAATGATGAATTTAAGCA
AATATTGGAACAGAAGATGGTCTCGGGTCCAATCTTCTTTATGCAAAATAAAAAAGGCGGTCAAGCAACTCAAATCAAGAGTTGGATTGATCGTCTTTTG
GTATCTGAGGATTAGTTATTTCCCAGACACTTTTC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1509 bp	502 aa	150	1658	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MYQNYITGQTALTLNLDFTIPSNHLANIISQFVDSIPEDVLLGKTAKTGRPAYHPAMMLKILLFAYSRRVFSGRKIELMLEENVPMMVLADHQKISYHTI
NNFRSSNHANELVKKSFLYFTNLLEAEGLINEGAVFIDGTKIEADANRYTFVWRKAVEKFHKKLKGQAVELYDELIAKEVVKAMAQEKVRTSQGLAELAQ
DTEAEIEKLTKKIEQEPRAIPGGSPRKARRRRLKKLLHKLRKDYVPRMKKYEEAEEIFAGRNSYSKTDHDATFMHMKEDHMKNGQLKPGYNIQAATTNQY
VVDFALYHNPTDFRTLEPFLKQMSTLDKFDKIVADAGYGSEDNYSMLEKEHPDKKYYIPYTMYEKEKTRKYRSDPTRLANWFYDEKDDYYLDQNGVRFNF
KYYSRRKDRSTDQVRDFKVYEADEFQLTPELERLAKTKSGRQRQVRYNPNWQYLKEKAKEVLQSPEGRHIYSMRKYDVEPIFGHLKNIFGIRRTHLRGKK
EG

Blast result :

Comments

ISLac1 is 62% aa simialr to IS663. There are 2 copies in Lactobacillus acidophilus NCFM genome, at coordinates : complement(77565-79397) and 1916505-1918337. The transposase of the first copy is longer than the IS sequence, it use the first STOP codon after the IRR.

References

1] ISfinder annotation (2005)
2] Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R., Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T., Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R. (2005) Proc. Natl. Acad. Sci. U.S.A. In press