ISPpu16
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_002947 | ND | Pseudomonas putida | Pseudomonas putida KT2440 |
DNA section
IS Length : 1677 bp
Ends
IR Length : 15/16
IRL : CCAGACTGTGTGAAAACGCCCGCGGTTGTCTAACCTTCTGGTCGTCGTGA
IRR : CCAGGCTGTGTGAAAACACGCGAAATGCGTTGAAGGGCGCAGGGATCTAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTGCAAAGG | GT | CGATAGCGGC | 2 |
DNA sequence
CCAGACTGTGTGAAAACGCCCGCGGTTGTCTAACCTTCTGGTCGTCGTGACCGTGGGGACAATCATGAAGCGATTCATCGAAGGTGAGGCTCGAACGCAG
GTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCG
ATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTC
CAGCCGCCGGCTTGAGCGTGAAGCTGAACGCAATGTCGAGTTGATGTGGCTCACAGGACGTCTGGCTCCGGATTTCAAAACCATCGCCGACTTTCGCAAG
GACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCA
AGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGC
GATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACAGATGCAGAAGCTC
AAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCA
CCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAA
CATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGACCGGGGCTATTACACAGGCACCGAAATCGTTGCATGTGAGCAA
GCTGGAATCTCGCCCTTCGTACCGAAACCACTGACCTCTAGCAGCAAAGCCGAAGGTCGATTTGGCAAGCAGGATTTCCTGTACGTCTCGACATCGGATG
AGTATCGATGCCCTGCGGGGCAATTGCTGACCAAGCGTTACTCAACGTGGGAAGACGGGATGCTGATGCATGTTTATTGGTTCTCGGGCTGCCAGTCCTG
CGCGATGCACAAGCAATGTACGACGGGCAAAGAGCGGCGGCTGAAACGCTGGGAACATGAAGCGGTCCTCGACTCGATGCAGGTTCAACTGGAGCACGAT
CCTGGGAAAATGAAGGTGCGCCGCCAAACCGTTGAGCACCCGTTCGGAACGCTCAAGTACTGGATGGGGGCTACCCACTTCCTGACCAGGACCCTGCCAC
GGGTTAGCACTGAAATGAGCCTTCATGTGCTCGCTTACAACCTCAAGCGAATGATGAGCATCTTGGGCATCAAGGGGCTGCTCAAGGCGATCAAGGCGTG
AACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGC
AATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGG
GTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCG
ATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTC
CAGCCGCCGGCTTGAGCGTGAAGCTGAACGCAATGTCGAGTTGATGTGGCTCACAGGACGTCTGGCTCCGGATTTCAAAACCATCGCCGACTTTCGCAAG
GACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCA
AGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGC
GATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATGGCGAAGGCCGAGCGGCTGAAAGAGAAGATCGAAACGCTGAAACAACAGATGCAGAAGCTC
AAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCA
CCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAA
CATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGACCGGGGCTATTACACAGGCACCGAAATCGTTGCATGTGAGCAA
GCTGGAATCTCGCCCTTCGTACCGAAACCACTGACCTCTAGCAGCAAAGCCGAAGGTCGATTTGGCAAGCAGGATTTCCTGTACGTCTCGACATCGGATG
AGTATCGATGCCCTGCGGGGCAATTGCTGACCAAGCGTTACTCAACGTGGGAAGACGGGATGCTGATGCATGTTTATTGGTTCTCGGGCTGCCAGTCCTG
CGCGATGCACAAGCAATGTACGACGGGCAAAGAGCGGCGGCTGAAACGCTGGGAACATGAAGCGGTCCTCGACTCGATGCAGGTTCAACTGGAGCACGAT
CCTGGGAAAATGAAGGTGCGCCGCCAAACCGTTGAGCACCCGTTCGGAACGCTCAAGTACTGGATGGGGGCTACCCACTTCCTGACCAGGACCCTGCCAC
GGGTTAGCACTGAAATGAGCCTTCATGTGCTCGCTTACAACCTCAAGCGAATGATGAGCATCTTGGGCATCAAGGGGCTGCTCAAGGCGATCAAGGCGTG
AACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGC
AATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1437 bp | 478 aa | 65 | 1501 | + | No |
Chemistry : DDE
ORF sequence :
MKRFIEGEARTQVTLLPECLDDYVTDENPVRVVDVFVDELDLGALDFAGVDPAATGRPAYHPAVLLKIYIYGYLNRIQSSRRLEREAERNVELMWLTGRL
APDFKTIADFRKDNGKAIRSVCRQFVILCRNLNLFSESIIAIDGSKFKAVNNRDRNFTQAKVKARMQQIEQSIERYLTAMDSADRAMPEVAMAKAERLKE
KIETLKQQMQKLKDIETQLLQTPDKQISLTDPDARSMSVGGRGSGTVGYNVQTAVDDQHHLIIAHEVTNVGHDRGQLSNMAKQARDQIGAESLTVVADRG
YYTGTEIVACEQAGISPFVPKPLTSSSKAEGRFGKQDFLYVSTSDEYRCPAGQLLTKRYSTWEDGMLMHVYWFSGCQSCAMHKQCTTGKERRLKRWEHEA
VLDSMQVQLEHDPGKMKVRRQTVEHPFGTLKYWMGATHFLTRTLPRVSTEMSLHVLAYNLKRMMSILGIKGLLKAIKA
APDFKTIADFRKDNGKAIRSVCRQFVILCRNLNLFSESIIAIDGSKFKAVNNRDRNFTQAKVKARMQQIEQSIERYLTAMDSADRAMPEVAMAKAERLKE
KIETLKQQMQKLKDIETQLLQTPDKQISLTDPDARSMSVGGRGSGTVGYNVQTAVDDQHHLIIAHEVTNVGHDRGQLSNMAKQARDQIGAESLTVVADRG
YYTGTEIVACEQAGISPFVPKPLTSSSKAEGRFGKQDFLYVSTSDEYRCPAGQLLTKRYSTWEDGMLMHVYWFSGCQSCAMHKQCTTGKERRLKRWEHEA
VLDSMQVQLEHDPGKMKVRRQTVEHPFGTLKYWMGATHFLTRTLPRVSTEMSLHVLAYNLKRMMSILGIKGLLKAIKA
Blast result :
Comments
ISPpu16 is 82% aa similar to ISPa7. There is only one copy in the Pseudomonas putida KT2440 genome.
References
1] ISfinder annotation (2005)
2] Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D., Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L., Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R., Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P., Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T., Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J., Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K., Duesterhoft,A., Tummler,B. and Fraser,C.
(2002) Environ. Microbiol. 4 (12), 799-808
2] Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D., Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L., Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R., Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P., Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T., Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J., Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K., Duesterhoft,A., Tummler,B. and Fraser,C.
(2002) Environ. Microbiol. 4 (12), 799-808