ISFnu2

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003454 ND Fusobacterium nucleatum
Fusobacterium nucleatum subsp. nucleatum ATCC 25586
DNA section
IS Length : 1776 bp

Ends


IR Length : 16

IRL : GGGGCTGTTGCAAATTAAATAGTTGAGTCCCAAAAGAAAAAAGATGCTAA
IRR : GGGGCTGTTGCAAATTGATAATTTTTTATCATCAATTTATGACAGCTTTT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGGGCTGTTGCAAATTAAATAGTTGAGTCCCAAAAGAAAAAAGATGCTAAAAAACAATTTTTTGTAGTTTAGCATCTTTTTAAATTTAAAAAGTATATTA
CATAAATTTTTTAGAAATTTTCTTCAAAATTTAATATAATATACTTATGATAAAGTCAACCAATAATAACAGATTTTTTAAGTTTTTTCAACCTAAACTT
TTTTACATTAATAAAGATATTGATAAAGATGATCCTGTTAGGCTGTTTAGCACCATTCTGGAGGAAATGGATTTTTCTAATTTAATGCAAGTATTCCCTA
ACAAAACTAAGGTACATCCTGTTAATATGTTTGCCATAATTATCTATGCTTACTCAAGAGGAATTTATTCTACTAGAGATATTGAGTATCTTTGTAAAGA
TAGCCAAAGAGCTCAATATTTATTAAATTCACATAATATCCCTGATTATTCCACTATTGCGCGCTTTCTTTCAAAAGCTACTGATGTTATCCATGAGTTG
TTCACTCAATTTGTAGAGAAGCTTTTTAAATTAAGTGAAATTTCTACTGAAACTATTTACATTGATGGTACTAAAATAGAAGCTTATGCTAATAAATATA
GTTTTGTTTGGAAGAAATCCACTTTAAAATATAAAGAAAGACTTGAAGAGAATATATTAGAGTTAATTGATGACTTTAATAGATATTTTAATAAAGATTT
AGATAATATTTTTGGTGTTTTTTCATATTTAGAAAATTTAAATATTCAAAAAGTACATGGTAAAGGTAAAAGAAAAAATAAAGAACAGGTATTGTTAGAA
AAAGCAGAATCATTTATTGAAAGATTTGAAAAATACACAAATTATCTAGAAATTTTAGGTGAAAGAAATAGTTTTTCTAAAATAGATAAAGATGCAACAT
TTATGAGAATGAAGGAAGATTACATGCGCAATGGTCAATTGAAACCAGGATATAATCTTCAAATTGGAGTTATTAGTGAATATATAGCTTCTTATGAAAT
TTTTCATAATCCTTCAGACTCTAAAACTCTAATTCCATTTCTTGAAAAAATTAAGTCTCAAAATATTGAAATTCAAAATGTTGTGGCTGATGCTGGTTAT
GAAAGTCTTCCTAACTATGAATACTTAGAAACTAATAATTATGTATCATACATAAAACCAATATACTATGAAAAATCAAAGACAAGAAAGTATAAAAAAG
ATTTGAATAAAGTAGAGAATTTAGATTATGATGAAAAAGAAAATAGATTATTTAGAAAAGATGGACTAGAATTAGAATTTCTCAACTATTCAAAAGATAA
AAAGTCAATATATTTTAAAAATCCAGAAACAGAAAAGATAGTGAGATGTAATAAAGAATTTAGAAGATTATCAAAGATATCAAAAAGTAACATTGAAACT
GAAATAGGAAAGCAACTTAGGATGAATAGAAGTATTCAAGTAGAAGGAGCTTTTGCAGTATTAAAAGAAGATATGAAATTGCGCAAATTAAAAGTTAGAG
GAAAAGAGAGTGCAAAGAGAGAGATAGGACTATTTTGTATAGCCTATAATTTTAATAGATACCTTGCAAAATTAATTAGAAAAAAGCAAGGAGTAATATT
GCATCCATTAAAAACAGCTTAAAAAAGTAAAAAATAATGGACTTGTATTAAGTTCGCCAAAAAACAAAATAGAAATCAAAATAAGATAAAATTCTAAAAG
ATTTCTATTTTTTTATTGAGAAAGAAAAAAGCTGTCATAAATTGATGATAAAAAATTATCAATTTGCAACAGCCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1476 bp491 aa1471622+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIKSTNNNRFFKFFQPKLFYINKDIDKDDPVRLFSTILEEMDFSNLMQVFPNKTKVHPVNMFAIIIYAYSRGIYSTRDIEYLCKDSQRAQYLLNSHNIPD
YSTIARFLSKATDVIHELFTQFVEKLFKLSEISTETIYIDGTKIEAYANKYSFVWKKSTLKYKERLEENILELIDDFNRYFNKDLDNIFGVFSYLENLNI
QKVHGKGKRKNKEQVLLEKAESFIERFEKYTNYLEILGERNSFSKIDKDATFMRMKEDYMRNGQLKPGYNLQIGVISEYIASYEIFHNPSDSKTLIPFLE
KIKSQNIEIQNVVADAGYESLPNYEYLETNNYVSYIKPIYYEKSKTRKYKKDLNKVENLDYDEKENRLFRKDGLELEFLNYSKDKKSIYFKNPETEKIVR
CNKEFRRLSKISKSNIETEIGKQLRMNRSIQVEGAFAVLKEDMKLRKLKVRGKESAKREIGLFCIAYNFNRYLAKLIRKKQGVILHPLKTA

 

Blast result :
Comments
ISFnu2 is 50% aa similar to IS1182 and 93% aa similar to ISFnu1.
There is 1 full copy of ISFnu2 in Fusobacterium nucleatum subsp. nucleatum genome.
References
1] ISfinder annotation (2004)
2] Kapatral,V., Anderson,I., Ivanova,N., Reznik,G., Los,T., Lykidis,A., Bhattacharyya,A., Bartman,A., Gardner,W., Grechkin,G., Zhu,L., Vasieva,O., Chu,L., Kogan,Y., Chaga,O., Goltsman,E., Bernal,A., Larsen,N., D'Souza,M., Walunas,T., Pusch,G.,Haselkorn,R., Fonstein,M., Kyrpides,N. and Overbeek,R. (2002) J. Bacteriol. 184 (7), 2005-2018