ISCac1

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003030 ND Clostridium acetobutylicum
Clostridium acetobutylicum ATCC 824
DNA section
IS Length : 1861 bp

Ends


IR Length : 11/15

IRL : GGGGTTGTTTCAAAACTAATTTAGTTTTGAAATGGCTCCTTTTCTATATA
IRR : GGGAGTGTCGCAAAATGATTAAAATTAATCATGAGCGATGCTCTTTTTTA

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGGGTTGTTTCAAAACTAATTTAGTTTTGAAATGGCTCCTTTTCTATATATAAAAAATGTGAGTTCCGAAGAACTCACATTAATTGTATAATTAAGCTAT
GCTAGTAACGAAATTACACACAAAAAATTATAATCAATTTAATGATAATTTGCAACTTATATTACCATTAAATTTAGAAAACTTAATACCGGAAGATGAT
TCTGTTCGCTTGCTAAGCTATTTATTGGAGGGATTAAATTATAAAAAATTGTACAAAGCGTATTCTTCCTTAGGAAGAAAATCAGCAGTTGAACCAAAAA
TCATGTTCAAAATAATATCCTATGCGTATTCGCAAAATATTTATTCAAGTAGAAAAATAGAAAAAGCATGCAAAAGGGATATAAATTTCAAGTGGCTACT
TCAAAGCTTTAAAGTACCTGATCATGCTACTATAAGTAGATTCAGAAAAAAATACCTTTCAAATGAAGTAATTGAAGATCTATTTTATCAACAAGTTAAC
TATTTAGCTAAGGAAAAAGAATTATTATTTGAAAATGTATTTATCGATGGTACTAAAATTGAGGCAAATGCTAATCGATACACTTTTGTTTGGAAGAAAG
TTATTTATAAAAATGAGGGCAAGATGTTTGATAAAATTATTGCTCTTGTTGAAGATATTAATCTTGAAAACTTAAAAGGATTTACTATTGGAAAAGAAAC
TTTGATAGATGATATAAATAAAATTCTTCAATGGCTTTTATTTGAAAAAGAAAAAAGAAATATAGAATTTGTTCATGGAATCGGTAAAAGAAAAACTAAA
ATTCAGAAATGGATAGAACAACTATCTCAATATAAAGAAAGACAAGAAAAATATAATTTAAGTAAGAAAATATTTTCAAAAAGAAATAGCTATTCTAAAA
CTGACAATGATGCAACTTTCATGCATATGAAAGATGATCATATGAGAAATGGTCAATTAAAACCTGCCTATAATGTACAAATAGCAGTTGATAGTGAATA
TGTAACTGGTGTTGGAGTATTTGATGATAGAAATGATATAGCAACATTAATACCAATGATTACTAACATGCAAAAAAAATTGGTCATACATACACCAATG
TGATTGCGGATTCTGGTTATGAAAGTGAAGAAAACTATTTGTTTTTAGAGTCTAATAATCAAATCCCATATATAAAACCTCAAACTTATGAGAAGTGGAA
AAAAAGAAGTTTTAAAAATGATATCAGTAGACGTGAAAATATGGAATATGATGCTAAAACAGATACATATACTTGCCATAATAATAGAAAATTATCACCA
TCATGTATTATTCATAAAAAATCTGCAAGTGGCTATACATCTGAGGTTACTGTTTATGAATGTGAAATTTGTGATAACTGCACTTTGAAACCAAAGTGCA
CAAAAGCAAAGAAGAATCGAAAAATGCAGGTTTCAAAGGCTTTTATTAAAAAGCGTGAAATTTCCTACAACAATATCAAAACTGAATTGGGAACTAAATT
AAGAATGAACAGATCTATTCAAGTTGAAGGTGCATTTGGAGTTTTAAAAAGCGACTATGAATTCAAAAGATTTTTAACACGTGGAAAAAATAGTGTGAAA
ACCGAATTTATTTTGCTTTGTTTTGGATATAACATTAACAAATTACACTCGAAAATTCAAAATAAAAGAACTCAAAAGTATCTTCATGAATTAAAGTCTA
TTTCCTAATTATGGAATAATATGATTAGGTTTATTTTAGTGCGCTAAAATATCATGGAAATTCAAATAATTAATTTAGCATTTAAAATATTTGGAGATTT
TGTAACTTAAATAAAAAAGAGCATCGCTCATGATTAATTTTAATCATTTTGCGACACTCCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
1005 bp334 aa991103+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MLVTKLHTKNYNQFNDNLQLILPLNLENLIPEDDSVRLLSYLLEGLNYKKLYKAYSSLGRKSAVEPKIMFKIISYAYSQNIYSSRKIEKACKRDINFKWL
LQSFKVPDHATISRFRKKYLSNEVIEDLFYQQVNYLAKEKELLFENVFIDGTKIEANANRYTFVWKKVIYKNEGKMFDKIIALVEDINLENLKGFTIGKE
TLIDDINKILQWLLFEKEKRNIEFVHGIGKRKTKIQKWIEQLSQYKERQEKYNLSKKIFSKRNSYSKTDNDATFMHMKDDHMRNGQLKPAYNVQIAVDSE
YVTGVGVFDDRNDIATLIPMITNMQKKLVIHTPM

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
570 bp189 aa11391708+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MFLESNNQIPYIKPQTYEKWKKRSFKNDISRRENMEYDAKTDTYTCHNNRKLSPSCIIHKKSASGYTSEVTVYECEICDNCTLKPKCTKAKKNRKMQVSK
AFIKKREISYNNIKTELGTKLRMNRSIQVEGAFGVLKSDYEFKRFLTRGKNSVKTEFILLCFGYNINKLHSKIQNKRTQKYLHELKSIS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1610 bp536 aa991708+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

 

Blast result :
Comments
ISCac1 is 55% (ORF1) and 52% (ORF2) aa similar to IS663.
References
1] ISfinder annotation (2004)
2] Nolling,J., Breton,G., Omelchenko,M.V., Markarova,K.S., Zeng,Q., Gibson,R., Lee,H.M., Dubois,J., Qiu,D., Hitti,J., Wolf,Y.I., Tatusov,R.L., Sabathe,F., Doucette-Stamm,L., Soucaille,P., Daly,M.J., Bennett,G.N., Koonin,E.V. and Smith,D.R.(2001) J. Bacteriol. 183 (16), 4823-4838