ISAzba7

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum brasilense
Azospirillum brasilense
DNA section
IS Length : 852 bp

Ends


IR Length : 15/17

IRL : GAGTGCGTAACAAAACCGGCCAGGAACGGTTGGTAGGGGCATGGCAGCCC
IRR : GAGCGCCTAACAGAACCATCTTTGGACGAAGCGCCAGCAGATGAGGCTGG

Insertion site


Left flankDirect repeatRight flankDR Length
CTATGCCTTATAATTAGGCACTCTAA0
TGGAACGGCAGTTGGGTTAAGAGTGCAGATCAAAACC3
GTTCCTTCCGATGAGCATAATGAAACGCTCTCAGGGGAGT0

DNA sequence

GAGTGCGTAACAAAACCGGCCAGGAACGGTTGGTAGGGGCATGGCAGCCCCTCTTGTTTCCGACGCCTTGTGGGCGATCATCGAACCGCTGATCCCGCCG
GAGCCGCCCAAGCCAAAAGGCGGACGGCCCCGGTTGGACGATCGTGCGGCGCTGACCGGCATCCTGTTCGTGCTGCGCACGGGCATCCCTTGGGAGCTTC
TGCCGGTGGAGATGGGCTGCGGCTCGGGGATGACCTGCTGGCGGCGTCTGCACGAGTGGCATCGGGCCGGGGTATGGGAGCGACTGCATCGCGTGCTGCT
CGACCGGCTCGGCTATGCCAACGCCATCAACTGGGATCGGGCGGCGGTGGACAGCGCCAGCGTTCCAGCAAAAAGGGGGGCGAGGAGACTGGCCCGAACC
CGACGGATCGCGGCAAGCCGGGCTCCAAGCGTCACATCCTCGTCGATGCCAATGGCATCCCACTCGCCCTGAAGATTTCGCCGGCCAACCGGCACGATAG
CAAGCTGCTGGAGACGCTGGTCGATGCCGTGCCGGCGATCCGCCAGTGTGCGGGCCGGCCACGGCGCCGCCCGGCCAAGTTGCACGCCGACAAGGGCTAC
GACTTTGCACACTGCCGGCGGGCGCTGCGCCAACGAGCGATCATTCCGCGGATCGCCCGGCGTGGCGTCGAGAGCAGCGAGCGTCTTGGCCGACACCGAT
GGGTGGTCGAGCGGACGCTCGCATGGTTCGCCCGCTTCCGCCGCATCGCCGTCCGATACGAACGGCGCGCCGACATCTTCACCGCCTTTCACCACATCGC
CGCCAGCCTCATCTGCTGGCGCTTCGTCCAAAGATGGTTCTGTTAGGCGCTC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
432 bp143 aa41472+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAAPLVSDALWAIIEPLIPPEPPKPKGGRPRLDDRAALTGILFVLRTGIPWELLPVEMGCGSGMTCWRRLHEWHRAGVWERLHRVLLDRLGYANAINWDR
AAVDSASVPAKRGARRLARTRRIAASRAPSVTSSSMPMASHSP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
777 bp258 aa70846+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

VGDHRTADPAGAAQAKRRTAPVGRSCGADRHPVRAAHGHPLGASAGGDGLRLGDDLLAASARVASGRGMGATASRAARPARLCQRHQLGSGGGGQRQRSS
KKGGEETGPNPTDRGKPGSKRHILVDANGIPLALKISPANRHDSKLLETLVDAVPAIRQCAGRPRRRPAKLHADKGYDFAHCRRALRQRAIIPRIARRGV
ESSERLGRHRWVVERTLAWFARFRRIAVRYERRADIFTAFHHIAASLICWRFVQRWFC

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
806 bp268 aa41846+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAAPLVSDALWAIIEPLIPPEPPKPKGGRPRLDDRAALTGILFVLRTGIPWELLPVEMGCGSGMTCWRRLHEWHRAGVWERLHRVLLDRLGYANAINWDR
AAVDSASVPAKKGGEETGPNPTDRGKPGSKRHILVDANGIPLALKISPANRHDSKLLETLVDAVPAIRQCAGRPRRRPAKLHADKGYDFAHCRRALRQRA
IIPRIARRGVESSERLGRHRWVVERTLAWFARFRRIAVRYERRADIFTAFHHIAASLICWRFVQRWFC

 

Blast result :
Comments
ISAzba7 is 77% aa similar to ISMch2. The tird ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)