ISSpi4
- Family IS3
- Group IS3
Isoform Synonym(s) ISSwp4
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011566 | ND | Shewanella piezotolerans | Shewanella piezotolerans WP3 |
DNA section
IS Length : 1258 bp
Ends
IR Length : 25/36
IRL : TGTAGTGGTACGGTGAATTTGGCCACCTAGTTAGAGGTGATATCATTACC
IRR : TGTAGTGGTCAACTAATATTGGCCACGATCTTAGAGTTTAACCAAAACAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTTTGTCTT | ATGTAATGTA | 0 | |
| ATTGAACAAG | GAG | GTTGCATGCC | 3 |
| GCTTGAATCC | AGA | TGAAAGCAAG | 3 |
| TTGTTCGATT | ATGGGGCGCA | 0 | |
| TGAATGGTCA | AAC | TTTTAATGCA | 3 |
DNA sequence
TGTAGTGGTACGGTGAATTTGGCCACCTAGTTAGAGGTGATATCATTACCTCATAAGTAAACAGGTGACACTATGACGAAACGTACAAGAAGACTTTTTA
GCGCAGAATTCAAACTTGAATCAGCTCAATTAGTGCTAGATCAAAATTACTCAATTGTTGAAGCCGCTCAAGCTATGAACGTGGGTAAATCGACCATGGA
TAAGTGGGTTCGGCAATTAAGAGAAGAGCGACAAGGTAAGCAACCTAAAGCATCACCTATATCACCAGAACAAATTGAAATTCGAGAGTTAAAAAAGCAA
CTAGCTCGCCTCCAGGAGCACAACGAAATATTAAAAAAAGCCACAGCTCTGTTGATGTCGGACTCACTGAACAATTCTTAATAATTGAGAAACTCAGGCA
GAGCTACAGCATAAAAACATTATGCGAAGTGTTCAGTATTCATCGTAGTAGTTACAAGTATTGGAGAAAACGGCCAATAACCATAAACGCAGATAAAGTA
AAGCTTCGAAGTCTAGTTAGCGAAGTACATACTGCAAGTAACGGCTCGGCAGGAGCTAGGACGGTTGCAGATATGGTTAGCCAGCAAGGTATCTCGTTAA
GCCGTTATCGCGCCACTAAGCTGATGAAGGAGCTTGGTATAGTGAGTTGTCAGGTACCTAAGCACAGATACCGAAAAGCGACACTAGAGCACATCGAAAT
CCCTAATCACCTAGGTCGACAATTCGCTGTCACCGCTCCGAATGAAGTTTGGGTTGGTGATGTCACCTACGTGTGGGCAGGTAACCGCTGGATGTACTTA
GCGGTTGTGATTGATCTATTTGCTCGTAAAGTCATTGGCTGGGCTATGTCGTTATCACCTGATAGCCGTTTAACGGGCAAAGCGCTTTCGATGGCCTATG
AAGGTCGTGGAAAACCTAAAGGTGTTATGCTCCATAGCGATCAAGGGACTCACTATACAAGCAGAAGATATCGACAACTATTATGGCGATATCAGCTCAA
GCAAAGTTTATCTCGACGTGGGAACTGTTGGGATAATAGTCCAATGGAACGCTTTTTTAGGAGCCTTAAGACAGAATGGATACCACCAATAGGGTATCGT
AGCTTTGCTGAAGCTCAGCAAGAAATTACACGATACATAATTGGATATTATTGTCAGCTTCGCCCGCACCAATATAACGGCGGACTGACACCTAATGAAT
CAGAACGATTGTTTTGGTTAAACTCTAAGATCGTGGCCAATATTAGTTGACCACTACA
GCGCAGAATTCAAACTTGAATCAGCTCAATTAGTGCTAGATCAAAATTACTCAATTGTTGAAGCCGCTCAAGCTATGAACGTGGGTAAATCGACCATGGA
TAAGTGGGTTCGGCAATTAAGAGAAGAGCGACAAGGTAAGCAACCTAAAGCATCACCTATATCACCAGAACAAATTGAAATTCGAGAGTTAAAAAAGCAA
CTAGCTCGCCTCCAGGAGCACAACGAAATATTAAAAAAAGCCACAGCTCTGTTGATGTCGGACTCACTGAACAATTCTTAATAATTGAGAAACTCAGGCA
GAGCTACAGCATAAAAACATTATGCGAAGTGTTCAGTATTCATCGTAGTAGTTACAAGTATTGGAGAAAACGGCCAATAACCATAAACGCAGATAAAGTA
AAGCTTCGAAGTCTAGTTAGCGAAGTACATACTGCAAGTAACGGCTCGGCAGGAGCTAGGACGGTTGCAGATATGGTTAGCCAGCAAGGTATCTCGTTAA
GCCGTTATCGCGCCACTAAGCTGATGAAGGAGCTTGGTATAGTGAGTTGTCAGGTACCTAAGCACAGATACCGAAAAGCGACACTAGAGCACATCGAAAT
CCCTAATCACCTAGGTCGACAATTCGCTGTCACCGCTCCGAATGAAGTTTGGGTTGGTGATGTCACCTACGTGTGGGCAGGTAACCGCTGGATGTACTTA
GCGGTTGTGATTGATCTATTTGCTCGTAAAGTCATTGGCTGGGCTATGTCGTTATCACCTGATAGCCGTTTAACGGGCAAAGCGCTTTCGATGGCCTATG
AAGGTCGTGGAAAACCTAAAGGTGTTATGCTCCATAGCGATCAAGGGACTCACTATACAAGCAGAAGATATCGACAACTATTATGGCGATATCAGCTCAA
GCAAAGTTTATCTCGACGTGGGAACTGTTGGGATAATAGTCCAATGGAACGCTTTTTTAGGAGCCTTAAGACAGAATGGATACCACCAATAGGGTATCGT
AGCTTTGCTGAAGCTCAGCAAGAAATTACACGATACATAATTGGATATTATTGTCAGCTTCGCCCGCACCAATATAACGGCGGACTGACACCTAATGAAT
CAGAACGATTGTTTTGGTTAAACTCTAAGATCGTGGCCAATATTAGTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 73 | 381 | + | No |
Description : First part of the transposase
ORF sequence :
MTKRTRRLFSAEFKLESAQLVLDQNYSIVEAAQAMNVGKSTMDKWVRQLREERQGKQPKASPISPEQIEIRELKKQLARLQEHNEILKKATALLMSDSLN
NS
NS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 912 bp | 303 aa | 339 | 1250 | + | No |
Description : Second part of the transposase
ORF sequence :
SHSSVDVGLTEQFLIIEKLRQSYSIKTLCEVFSIHRSSYKYWRKRPITINADKVKLRSLVSEVHTASNGSAGARTVADMVSQQGISLSRYRATKLMKELG
IVSCQVPKHRYRKATLEHIEIPNHLGRQFAVTAPNEVWVGDVTYVWAGNRWMYLAVVIDLFARKVIGWAMSLSPDSRLTGKALSMAYEGRGKPKGVMLHS
DQGTHYTSRRYRQLLWRYQLKQSLSRRGNCWDNSPMERFFRSLKTEWIPPIGYRSFAEAQQEITRYIIGYYCQLRPHQYNGGLTPNESERLFWLNSKIVA
NIS
IVSCQVPKHRYRKATLEHIEIPNHLGRQFAVTAPNEVWVGDVTYVWAGNRWMYLAVVIDLFARKVIGWAMSLSPDSRLTGKALSMAYEGRGKPKGVMLHS
DQGTHYTSRRYRQLLWRYQLKQSLSRRGNCWDNSPMERFFRSLKTEWIPPIGYRSFAEAQQEITRYIIGYYCQLRPHQYNGGLTPNESERLFWLNSKIVA
NIS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1178 bp | 392 aa | 73 | 1250 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKRTRRLFSAEFKLESAQLVLDQNYSIVEAAQAMNVGKSTMDKWVRQLREERQGKQPKASPISPEQIEIRELKKQLARLQEHNEILKKSHSSVDVGLTE
QFLIIEKLRQSYSIKTLCEVFSIHRSSYKYWRKRPITINADKVKLRSLVSEVHTASNGSAGARTVADMVSQQGISLSRYRATKLMKELGIVSCQVPKHRY
RKATLEHIEIPNHLGRQFAVTAPNEVWVGDVTYVWAGNRWMYLAVVIDLFARKVIGWAMSLSPDSRLTGKALSMAYEGRGKPKGVMLHSDQGTHYTSRRY
RQLLWRYQLKQSLSRRGNCWDNSPMERFFRSLKTEWIPPIGYRSFAEAQQEITRYIIGYYCQLRPHQYNGGLTPNESERLFWLNSKIVANIS
QFLIIEKLRQSYSIKTLCEVFSIHRSSYKYWRKRPITINADKVKLRSLVSEVHTASNGSAGARTVADMVSQQGISLSRYRATKLMKELGIVSCQVPKHRY
RKATLEHIEIPNHLGRQFAVTAPNEVWVGDVTYVWAGNRWMYLAVVIDLFARKVIGWAMSLSPDSRLTGKALSMAYEGRGKPKGVMLHSDQGTHYTSRRY
RQLLWRYQLKQSLSRRGNCWDNSPMERFFRSLKTEWIPPIGYRSFAEAQQEITRYIIGYYCQLRPHQYNGGLTPNESERLFWLNSKIVANIS
Blast result :
Comments
There are 5 copies of ISSpi4 encoded in Shewanella piezotolerans (plus one partial copy). The transposase encoded by ISSpi4 has 84% identity to that encoded by ISVch4. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift.
References
1] Romine, M. (2009) Direct submission.
2] Wang,F., Wang,J., Jian,H., Zhang,B., Li,S., Wang,F., Zeng,X., Gao,L., Bartlett,D.H., Yu,J., Hu,S. and Xiao,X. (2008) PLoS ONE 3 (4), E1937
2] Wang,F., Wang,J., Jian,H., Zhang,B., Li,S., Wang,F., Zeng,X., Gao,L., Bartlett,D.H., Yu,J., Hu,S. and Xiao,X. (2008) PLoS ONE 3 (4), E1937