ISSpo7
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003911 | ND | Silicibacter pomeroyi | Silicibacter pomeroyi DSS-3 Ruegeria pomeroyi DSS-3 |
DNA section
IS Length : 843 bp
Ends
IR Length : 12/16
IRL : GGCTCCAGACCCATTGATTTCATACTTCTGGCGTGATTCAGGCTCCGCAA
IRR : GGCTCAGGACTCGTTCTCGGGTCATAACCAGAACAAAACGGTTGCGGCTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACGTCATAGCT | TA | CCGGATGCTCAGCAA | 2 |
DNA sequence
GGCTCCAGACCCATTGATTTCATACTTCTGGCGTGATTCAGGCTCCGCAAGGAGATCTGATCGATGAGCAATCTGTATTGGCTGACCGACGCCCAGATGG
AGCGCCTGAAGCCGTTCTTTCCCAAGAGCCATGGCAAGCCCCGCGTCGATGACGCGTCGCGTCCTCAGCGGCATAATCTTCATCAATCGCAATGGGTTGC
GTTGGTGTGACGCGCCGAAGGAATACGGCCCGGCCAAGACCCTCTACAACCGTTGGAAACGCTGGAGCGACAACGGGGTCTTCGCCCGGATCATGGTGGG
CCTGGCCACCGAAAGCGCCGAGCACAAGACGATCATGATCGACGCGACCTATCTCAAGGCACACCGCACTGCGTCGAGCCTGTGCGTGAAAAAGGGGGGC
GCGGGCGCCAAATCGGGCGCACCAAAGGCGGTATGAACACCAAGCTGCACGCCGTTGCCGATGCCAAGGGGCGGCCGATCGGGTTCTTCATGTCCGCCGG
GCAGGTCAGCGATTATACCGGCGCCGCGGCTCTTCTGGGCAGTCTGCCGAAGGCGGGTTGGCTGCTGGCCGACAGGGGCTATGACGCCGATTGGTTCAGA
GAAGCGTTGAAAGACAAGGGGATAAAGGTTTGCATCCCCGGCCGGAAATCCCGCAAGAAGGCCGTCAAATACGACAAGCGGCGCTACAAACGGCGCAACC
GGATCGAGATCATGTTCGGCCGGTTGAAGGATTGGAGGCGCGTCGCCACCCGATACGACCGGTGCGCGGAAACGTTCTTCTCCGCGATCATGCTAGCCGC
AACCGTTTTGTTCTGGTTATGACCCGAGAACGAGTCCTGAGCC
AGCGCCTGAAGCCGTTCTTTCCCAAGAGCCATGGCAAGCCCCGCGTCGATGACGCGTCGCGTCCTCAGCGGCATAATCTTCATCAATCGCAATGGGTTGC
GTTGGTGTGACGCGCCGAAGGAATACGGCCCGGCCAAGACCCTCTACAACCGTTGGAAACGCTGGAGCGACAACGGGGTCTTCGCCCGGATCATGGTGGG
CCTGGCCACCGAAAGCGCCGAGCACAAGACGATCATGATCGACGCGACCTATCTCAAGGCACACCGCACTGCGTCGAGCCTGTGCGTGAAAAAGGGGGGC
GCGGGCGCCAAATCGGGCGCACCAAAGGCGGTATGAACACCAAGCTGCACGCCGTTGCCGATGCCAAGGGGCGGCCGATCGGGTTCTTCATGTCCGCCGG
GCAGGTCAGCGATTATACCGGCGCCGCGGCTCTTCTGGGCAGTCTGCCGAAGGCGGGTTGGCTGCTGGCCGACAGGGGCTATGACGCCGATTGGTTCAGA
GAAGCGTTGAAAGACAAGGGGATAAAGGTTTGCATCCCCGGCCGGAAATCCCGCAAGAAGGCCGTCAAATACGACAAGCGGCGCTACAAACGGCGCAACC
GGATCGAGATCATGTTCGGCCGGTTGAAGGATTGGAGGCGCGTCGCCACCCGATACGACCGGTGCGCGGAAACGTTCTTCTCCGCGATCATGCTAGCCGC
AACCGTTTTGTTCTGGTTATGACCCGAGAACGAGTCCTGAGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 573 bp | 241 aa | 253 | 825 | + | No |
Chemistry : DDE
ORF sequence :
MERLKPFFPKSHGKPRVDDASRPQRHNLHQSQWVALV*RAEGIRPGQDPLQPLETLERQRGLRPDHGGPGHRKRRAQDDHDRRDLSQGTPHCVEPVREKG
GRGRQIGRTKGGMNTKLHAVADAKGRPIGFFMSAGQVSDYTGAAALLGSLPKAGWLLADRGYDADWFREALKDKGIKVCIPGRKSRKKAVKYDKRRYKRR
NRIEIMFGRLKDWRRVATRYDRCAETFFSAIMLAATVLFWL
GRGRQIGRTKGGMNTKLHAVADAKGRPIGFFMSAGQVSDYTGAAALLGSLPKAGWLLADRGYDADWFREALKDKGIKVCIPGRKSRKKAVKYDKRRYKRR
NRIEIMFGRLKDWRRVATRYDRCAETFFSAIMLAATVLFWL
Blast result :
Comments
ISSpo7 is 73% aa similar to ISRhsp4. There is a STOP codon in frame at position 38 of the transposase.
There are four copies of ISSpo7 in Silicibacter pomeroyi DSS-3.
There are four copies of ISSpo7 in Silicibacter pomeroyi DSS-3.
References
1] Moran,M.A., Buchan,A., Gonzalez,J.M., Heidelberg,J.F., Whitman,W.B., Kiene,R.P., Henriksen,J.R., King,G.M., Belas,R., Fuqua,C., Brinkac,L., Lewis,M., Johri,S., Weaver,B., Pai,G., Eisen,J.A., Rahe,E., Sheldon,W.M., Ye,W., Miller,T.R., Carlton,J., Rasko,D.A., Paulsen,I.T., Ren,Q., Daugherty,S.C., Deboy,R.T., Dodson,R.J., Durkin,A.S., Madupu,R., Nelson,W.C., Sullivan,S.A., Rosovitz,M.J., Haft,D.H., Selengut,J. and Ward,N. (2004) Nature 432 (7019), 910-913