ISBusp4
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_014120 | ND | Burkholderia sp. | Burkholderia sp. CCGE1002 plasmid pBC201 |
DNA section
IS Length : 1633 bp
Ends
IR Length : 19/20
IRL : GGCCGAGGGTGTGTGAAAACGCACTCATCGCCTAAACTGAATCAACGCAT
IRR : GGCCGAGGGTGTGTGGAAACCGTTCTACGGCTATGCAGGGGAAGTTGCCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACGCTGGTC | ACAAGCGGTC | 0 | |
| GACGCTTCAT | GGTCAGCGAC | 0 |
DNA sequence
GGCCGAGGGTGTGTGAAAACGCACTCATCGCCTAAACTGAATCAACGCATTGAGATCGGGTGACTCATGAAGCGATTCGTTGAAGGCGATGACCGCAAGC
AGGTCGCACTGCTTCCCGAATGCATCGATGACTACATCGGGCAGGACAATCCGGTGAGGATCATAGACGTGTTCGTCGACGAACTCGATCTTGCGGAACT
CGGCTTCGATGGGGCTTCGCCCGCTCTCACCGGTCGGCCGTCTTATCATCCTGCAGTGATGCTCAAGGTCTACATCTACGGGTATCTGAATCGCTTGCCT
TCCAGCAGGCGTCTTGAACGGGAATGCCAGCGAAATCTCGAGATGATGTGGCTGACGGGACGCCTGGCACCGGACTTCAAAACGATCGCCGACTTTCGCC
GCGACAATGGTGCCGCCATTCGCAACGTGTGCCGGCGCTTCGTCGAACTGTGCCGCGGCCTGAAGCTTCTTTCCAGCGACATGGTGGCCATCGACGGCAG
CAAGTTCAAAGCCGTAAACAGCCGCGACCGGAACTACACCGCTGGCAAGATCGACAAGCGTCAGCAGCAGATCGAGGAGAGTATCCAGCGGTACCTCGAC
GCGATTGAGACCGCGGATCGCGTCAGTCCGACCGGTTTCGATGTGAAGACCGTACGCCTGTATGAGAGGGTCGCTTTGCTGCGGCAACGGATGCGCGAGC
TCGAGCAGATCCGGAAGCAACTGAAGACGCAACCGGACAGACAGCTGTCACTGACAGATCCCGACTCGCGTTCGATGACCAGCAGCGGCAAAAAGACCGG
AACAGTCGGCTACAACGTACAGGCTGCCGTAGACACGAAACATCACCTGATCGTTGAACACGAGGTGACCAATGTCGGAGGAGATCACGGGCAGCTCAGC
AAGATCGCGCTCGCAGCAAGAGACGCGATGGGCAAATCCGGACTCAGGGTGCTAGCCGATCGGGGCTACTTCAGTGGCCCGGATATTCGTACCTGCGAAC
TCTCGGGTATCAAGGCATATGTACCGAAGCCGTTGACGTCTGCATCCCGAAAGAAAGGCCTTTTTACCAAGCGCGACTTCATCTACATCGCTAAAGACGA
TGTCTATCGTTGCCCGGCCGGCGAGCGCGCCATTCATCGGTTCACGACTGTCGAACATGGCATGAATCTGAAGGTGTACTGGCCCAGCGCATGTCCGCAT
TGCCATCTCAAGGAGCGATGTTCTCCAAGCGACTACCGACGCATTCGACGCTGGGAACACGAACAGATACTGGAAGCCATGCAGCGACGGCTCGACCGGA
ATCCAGACGCAATGACGATCCGGCGCAGCACCGTTGAGCATGTGTTCGGCACGCTCAAACATTGGATGGGTGCCACGCACTTCCAGACCAGAACCCTCGC
ACGAGTGAGCACCGAGATGAGCCTTCAGGTGTTGGCTTACAACCTGAAGCGAGTCATGAAGATACTCGGCGTTGCGAAAACGATGAGGGCGATACGAATG
GCTGGAAGCTGAGGTCCTGCGGGGCCTTCTTGATACTTACAACCTTAGACTGTCGCAAAGCCACCAACTACGGCCGGCCTGTCCGGCAACTTCCCCTGCA
TAGCCGTAGAACGGTTTCCACACACCCTCGGCC
AGGTCGCACTGCTTCCCGAATGCATCGATGACTACATCGGGCAGGACAATCCGGTGAGGATCATAGACGTGTTCGTCGACGAACTCGATCTTGCGGAACT
CGGCTTCGATGGGGCTTCGCCCGCTCTCACCGGTCGGCCGTCTTATCATCCTGCAGTGATGCTCAAGGTCTACATCTACGGGTATCTGAATCGCTTGCCT
TCCAGCAGGCGTCTTGAACGGGAATGCCAGCGAAATCTCGAGATGATGTGGCTGACGGGACGCCTGGCACCGGACTTCAAAACGATCGCCGACTTTCGCC
GCGACAATGGTGCCGCCATTCGCAACGTGTGCCGGCGCTTCGTCGAACTGTGCCGCGGCCTGAAGCTTCTTTCCAGCGACATGGTGGCCATCGACGGCAG
CAAGTTCAAAGCCGTAAACAGCCGCGACCGGAACTACACCGCTGGCAAGATCGACAAGCGTCAGCAGCAGATCGAGGAGAGTATCCAGCGGTACCTCGAC
GCGATTGAGACCGCGGATCGCGTCAGTCCGACCGGTTTCGATGTGAAGACCGTACGCCTGTATGAGAGGGTCGCTTTGCTGCGGCAACGGATGCGCGAGC
TCGAGCAGATCCGGAAGCAACTGAAGACGCAACCGGACAGACAGCTGTCACTGACAGATCCCGACTCGCGTTCGATGACCAGCAGCGGCAAAAAGACCGG
AACAGTCGGCTACAACGTACAGGCTGCCGTAGACACGAAACATCACCTGATCGTTGAACACGAGGTGACCAATGTCGGAGGAGATCACGGGCAGCTCAGC
AAGATCGCGCTCGCAGCAAGAGACGCGATGGGCAAATCCGGACTCAGGGTGCTAGCCGATCGGGGCTACTTCAGTGGCCCGGATATTCGTACCTGCGAAC
TCTCGGGTATCAAGGCATATGTACCGAAGCCGTTGACGTCTGCATCCCGAAAGAAAGGCCTTTTTACCAAGCGCGACTTCATCTACATCGCTAAAGACGA
TGTCTATCGTTGCCCGGCCGGCGAGCGCGCCATTCATCGGTTCACGACTGTCGAACATGGCATGAATCTGAAGGTGTACTGGCCCAGCGCATGTCCGCAT
TGCCATCTCAAGGAGCGATGTTCTCCAAGCGACTACCGACGCATTCGACGCTGGGAACACGAACAGATACTGGAAGCCATGCAGCGACGGCTCGACCGGA
ATCCAGACGCAATGACGATCCGGCGCAGCACCGTTGAGCATGTGTTCGGCACGCTCAAACATTGGATGGGTGCCACGCACTTCCAGACCAGAACCCTCGC
ACGAGTGAGCACCGAGATGAGCCTTCAGGTGTTGGCTTACAACCTGAAGCGAGTCATGAAGATACTCGGCGTTGCGAAAACGATGAGGGCGATACGAATG
GCTGGAAGCTGAGGTCCTGCGGGGCCTTCTTGATACTTACAACCTTAGACTGTCGCAAAGCCACCAACTACGGCCGGCCTGTCCGGCAACTTCCCCTGCA
TAGCCGTAGAACGGTTTCCACACACCCTCGGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1446 bp | 481 aa | 67 | 1512 | + | No |
Chemistry : DDE
ORF sequence :
MKRFVEGDDRKQVALLPECIDDYIGQDNPVRIIDVFVDELDLAELGFDGASPALTGRPSYHPAVMLKVYIYGYLNRLPSSRRLERECQRNLEMMWLTGRL
APDFKTIADFRRDNGAAIRNVCRRFVELCRGLKLLSSDMVAIDGSKFKAVNSRDRNYTAGKIDKRQQQIEESIQRYLDAIETADRVSPTGFDVKTVRLYE
RVALLRQRMRELEQIRKQLKTQPDRQLSLTDPDSRSMTSSGKKTGTVGYNVQAAVDTKHHLIVEHEVTNVGGDHGQLSKIALAARDAMGKSGLRVLADRG
YFSGPDIRTCELSGIKAYVPKPLTSASRKKGLFTKRDFIYIAKDDVYRCPAGERAIHRFTTVEHGMNLKVYWPSACPHCHLKERCSPSDYRRIRRWEHEQ
ILEAMQRRLDRNPDAMTIRRSTVEHVFGTLKHWMGATHFQTRTLARVSTEMSLQVLAYNLKRVMKILGVAKTMRAIRMAGS
APDFKTIADFRRDNGAAIRNVCRRFVELCRGLKLLSSDMVAIDGSKFKAVNSRDRNYTAGKIDKRQQQIEESIQRYLDAIETADRVSPTGFDVKTVRLYE
RVALLRQRMRELEQIRKQLKTQPDRQLSLTDPDSRSMTSSGKKTGTVGYNVQAAVDTKHHLIVEHEVTNVGGDHGQLSKIALAARDAMGKSGLRVLADRG
YFSGPDIRTCELSGIKAYVPKPLTSASRKKGLFTKRDFIYIAKDDVYRCPAGERAIHRFTTVEHGMNLKVYWPSACPHCHLKERCSPSDYRRIRRWEHEQ
ILEAMQRRLDRNPDAMTIRRSTVEHVFGTLKHWMGATHFQTRTLARVSTEMSLQVLAYNLKRVMKILGVAKTMRAIRMAGS
Blast result :
Comments
ISBusp4 is 77% aa similar to ISPpu16.
References
1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Martinez-Romero,E., Hernandez,M.A.R., Tiedje,J.M. and Woyke,T. (2010) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Martinez-Romero,E., Hernandez,M.A.R., Tiedje,J.M. and Woyke,T. (2010) Direct submission GenBank.