ISMdi27

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium dichloromethanicum
Methylobacterium dichloromethanicum DM4
DNA section
IS Length : 2476 bp

Ends


IR Length : 16/20

IRL : TGCAACTTTGACCCCGTGATGCGGGGGATCGGCGTCCAATTCTGACCCCC
IRR : TGGAATGTTGACCCCGGATCGGCGTCCAAAATTAACCCCCTGGATGGATG

Insertion site


Left flankDirect repeatRight flankDR Length
CTGTCAAACAGCGCCGAAACACAA2

DNA sequence

TGCAACTTTGACCCCGTGATGCGGGGGATCGGCGTCCAATTCTGACCCCCTGAAGCTTGCTCTCGCAGACTGCCATCCGGGACGACCGGACGGGTGGTGG
GGGATGAAGGGCGTGGACACGATTGCGCGCATCCGGCGCGAGTTCTTCATCCGCGGCCGGTCGATCAAGGACATCGTCCGCGACCTGCACGTCTCCCGTA
ACACCGTGCGCAAGGTCATCCGCTCGGGAGCCACCAGCTTTGCCTACGAGCGCGAGGTGCAGCCGCTGCCCAAGCTGGGACCATGGCGGGACGACCTCGA
CCGGATGCTGGCCACGAACGCCAGCCGGTCGCCGCGCGAGCGGCTGACCCTGATCCGGGTGTTCGAGGCGCTGCGCGGGCTCGGCTACGAGGGCGGCTAC
GACGCGGTGCGCCGCTACGCCAAGGGCTGGAAGCGGGAGCGCACCGCCATCACGGCGCCCGCCTTCGTACCGCTCTCCTTCGCGCCGGGCGAGGCCTACC
AGTTCGACTGGAGCCACGAGATCGTGCTGATCGGCGGCGTGACCACGACGGTCAAGGTCGCCCACGTCCGGCTCTGCCACTCGCGCATGTTGTTCGTGCG
CGCCTACCCGCGTGAGAGCCAGGAGATGGTCTTTGACGCCCATGACCGGGCGTTCGCCTTCTTCCGCGGCGCCTGCCAGCGCGGGATCTACGACAACATG
AAGACCGCGGTGGAGACGATCTTCGTCGGGCGCGAGCGCGCCTACAATCGCCGCTTCCTGCAGATGTGCTCGCACTATCTCGTCGAGCCGGTGGCCTGCA
CGCCGGCCTCGGGCTGGGAGAAGGGTCAGGTCGAGAACCAGGTCGGGCTGGTGCGCGAGCGCCTGTTCACCCCGCGCATCCGGGTGAAGAGCTACGACGA
ACTGAACGCCCTGCTGCTCGACGGGGTGATCGCCTACGCCAAGGCCCATCCTCATCCCGAGGAGCGCGAGCGCACCGTCTGGGAGCGGTTCGAGGCCGAG
CGGGCGGCGCTGGTGCCCTATGCAGGCCGCTTCGACGGCTTCCACGCCGTGCCGGCCGCCGTCTCCTCGACCTGCCTCGTGCGCTTCGACAACAACAAGT
ATTCGGTCGCCGCCTCGGCCATTGGCCGTCCGGTCGAGGTGCGCGCCTACGCCGAGCGTGTCGAGATCCGCCAGGACGGCCGCATCGTTGCCGAGCATGC
GCGCGCCTTCGGCCGTGGCCAGACGGTGTTCGATCCCTGGCACTACGTTCCCGTGCTCGCCCGCAAACCCGGCGCGCTGCGCAACGGGGCGCCGTTCAAG
GACTGGGTGCTGCCCGCCGCCCTTGACCGGATCCGGCGCAAGCTTACCGGCAGCGCCGACGGCGACCGCCAGATGGTCGAGATCCTCACCGCCGTGCTCG
GCGATGGCCTGCCCGCGGTCGAAGCCGCCTGTGCCGAGGCGCTGCGCGAGGGCGTCCACTCGGCCGACGTCGTCCTCAACATCCTGGCTCGCCAGCGCGA
GCCCGCTGCCGCCGTCACCCTCGCGACGCCCGAGGGTCTGCGGCTGCGCCACGAACCTGTTGCTGACTGCGCCCGCTACGACAGCCTGAGGAGAGCCCGA
TGATGGAACGCCAGCAGATCCTCGCCACGATGGGCGAGCTGAAGTTGTTCGGGATGAAGGCGGCCTACGACGAGATCATCAAGGTCGCGGTCAAGCGCAC
CCACGAGCCGCAGCAGATCGTCGGCGACCTGCTCCAGGCCGAGATCAGCGAGAAGCAGGCCCGCTCGATCCGCTACCAGATGACGATCGCCAAGCTGCCC
CTGGCCAAGGACCTCGCCGAGTTCGCCTTCGCCGGCACGCCGATCAACGAGGGGCTGGTGCGCGATCTCGCCGGCGGCGAGTTCCTGGCCCACCAGCGCA
ACGTCGTGCTGGTCGGCGGCACCGGTACCGGCAAGACGCATCTGGCCATCGCGATGGCCCGGGCCTGCATCCGAGACGGGGCACGAGCTCGGTTCTACAA
CGTGGTCGACCTCGTCAATCGGCTCGAAGCCGAGGCGCGGGCCGGCCGGCAGGGCCGCATCGCCGACCACCTCGCCCGCCTCGACCTCGTGGTGCTGGAC
GAGCTCGGCTACCTGCCGTTCGCACAGTCGGGCGGGCAACTCCTGTTCCACCTAATCAGCAAGCTCTACGAGACCACCTCGATCGTGGTGACCACCAACC
TCGCCTTCGGGGAGTGGCCGAGCGTGTTCGCCGGTGACGCCAAGATGACCACCGCGCTGCTCGATCGGCTCACACACCACTGCGAGATCGTCGAGACCGG
CAACGAGAGCTGGCGCTTCAAGAACCGCGCCTGAGGTCGGCTACCCGTCGCGCCTTCTGGATCCCTGATCAGCCCGGCTGCGCCACCTCGATCCGCTCCG
CCGGGCTGACCAGGGGCGTCACGACACATCCATCCAGGGGGTTAATTTTGGACGCCGATCCGGGGTCAACATTCCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1500 bp499 aa1041603+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKGVDTIARIRREFFIRGRSIKDIVRDLHVSRNTVRKVIRSGATSFAYEREVQPLPKLGPWRDDLDRMLATNASRSPRERLTLIRVFEALRGLGYEGGYD
AVRRYAKGWKRERTAITAPAFVPLSFAPGEAYQFDWSHEIVLIGGVTTTVKVAHVRLCHSRMLFVRAYPRESQEMVFDAHDRAFAFFRGACQRGIYDNMK
TAVETIFVGRERAYNRRFLQMCSHYLVEPVACTPASGWEKGQVENQVGLVRERLFTPRIRVKSYDELNALLLDGVIAYAKAHPHPEERERTVWERFEAER
AALVPYAGRFDGFHAVPAAVSSTCLVRFDNNKYSVAASAIGRPVEVRAYAERVEIRQDGRIVAEHARAFGRGQTVFDPWHYVPVLARKPGALRNGAPFKD
WVLPAALDRIRRKLTGSADGDRQMVEILTAVLGDGLPAVEAACAEALREGVHSADVVLNILARQREPAAAVTLATPEGLRLRHEPVADCARYDSLRRAR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
735 bp244 aa16002334+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MMERQQILATMGELKLFGMKAAYDEIIKVAVKRTHEPQQIVGDLLQAEISEKQARSIRYQMTIAKLPLAKDLAEFAFAGTPINEGLVRDLAGGEFLAHQR
NVVLVGGTGTGKTHLAIAMARACIRDGARARFYNVVDLVNRLEAEARAGRQGRIADHLARLDLVVLDELGYLPFAQSGGQLLFHLISKLYETTSIVVTTN
LAFGEWPSVFAGDAKMTTALLDRLTHHCEIVETGNESWRFKNRA

 

Blast result :
Comments
ISMdi27 is 95% (istA, the transposase) and 97% (istB, the helper of transposition) aa similar to ISMex39.
References