ISfinder Mirror Site

ISMdi18

Family IS1595
Group ISNwi1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Methylobacterium dichloromethanicum	Methylobacterium dichloromethanicum DM4

DNA section

IS Length : 3479 bp

Ends

IR Length : 23/24

IRL : GGCGATTATATCGTTGACACATACGGCAACCGCAGGTAGGTTTTAGCTCT
IRR : GGCGATTATATCGTTCACACATACACCTTGACCGACCACGACCGTTGGGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GAGGGCCGCT	TAATTTTT	AGAGGCGGAT	8

DNA sequence

GGCGATTATATCGTTGACACATACGGCAACCGCAGGTAGGTTTTAGCTCTAGGAGCTAAACCGATGTCCGCCCTTTCCGCTGCATTCTTCCACGACGAGG
CCGCCGCTTTCGCCAAGCTGGAAAGCCTTCTGTGGCCGGAAGGCCCGGTCTGCCCTCACTGCGGCGGCATGGGCCGGATCACGAACGTAAAGGGCGGTCG
CATGGGCCTGCGCCGCTGCGGCGACTGCAAGAAGCAGTTCACGGTGACGGTCGGCACGGTTTTCGAGTCCAGCCATGTGAAGCTGCACCTGTGGCTCCAG
GCTGCGCACCTTCTTGCGTCCAGCAAGAAGGGCTTCTCCGCGCACCAACTGCACCGGACCCTCGGCGTCACCTACAAGACCGCGTGGTTCATGTTCCATC
GCCTGCGTGAGGCGATGCGCGACGGCGCGCTTGCCCCGATGGGCGGTCTGGACGGCCCGGCCGGTGGCGCTGGCATCGTGGAAGCCGACGAGACGTTCAT
CGGCCGCGAGCCCGGCAAGCCGAAGAAGCGCGCCTATCACCACAAGATGAAGGTGCTGAGCCTTGTGGATCGCGACACGAAGCAGGTCCGCTCCGTCGTC
GTGGATGATCTCAAGCCCGACACGGTGAAGCCGATCCTCGCCGCGAACATCGCCAAGGAAGCCAGCCTCTTCACCGACGAGGCGGGCCACTACGTGAAGC
TCGGCAAGGACTTCATTGAGCATCAGATCACGTCTCACGGCAAAGGCGAGTACGTGCGCGGCATCGTCCATACGAACACCGTCGAAGGCTACTTCTCCGT
CTTCAAGCGCGGCATGAAGGGTGTCTATCAGCACTGCGGCAAGCAGCACTTACACCGCTACCTCGCGGAGTTCGATTTCCGGTACAACAACCGGGTGAAG
CTCGGTGTGGGCGATGTCGAGCGGGCCGAGCGGGCGCTTCGGGGCGTCGTTGGGAAGCGCCTTACGTACCAAACAACTCGTTGACCGGAGACGGCGTTAA
CCCCATATTCCCCCCTGTCCCCGAGCGCGAAAACCTGGGAGATATCCTGGACTGCCGCAGTAGCGGCTAGGCTCGGGGGCCGCGCTTTAAGTTGAACTTC
GGGCGCACATTCCAAACAGATAATCGGTGTTGCGAAATTGAAGTGCTGGCCTCTAAGCTATGCAGCTTCGTTTTTTCGAACGCATAGATCGCTAACTTGC
GTTTCATATCGCTCGTTTCTGGCAAAAGGTGCCTACCGTTTCGATATGCGGTCAATGCACCTAGCGTTACATCTAAGAACTGAAGAAATGGCGTTTGCTT
AGAGTCTCTGGGTTCAATTGTTCGGACACAATTAGGCGGATGATTGAACTCGCTTACAGCGCCTTCGTTCAGCTTTTGCTTATAGCCCGGCAATAGAGAC
GTGCAGTTGCCATTATCGGGGCGAATGTGGATGTCATACTCCGCGCCGTAGTACCTAACTGCGCGGTGCAGCATTAATTGATAATGGGCCTTACTAACAG
TGTCTACAAGCTTGCGCTCTCCACTGATACTATGATCATACTTGCCCATCTCTTGAAATCGAATGTGGAAGTTGACGATATTTGTCTCTATTAGCTTAAA
AAGCAAATCAATATAAGCAATATGGGCGCTATCTCGGCGTTTTTTGGCTGTAGCCCATTTGATCTCTGAAGTAATTTCATAATTGTAATTAATTTTCTGA
ATATCTGATAATATATAATCAGCCATTCCCAAATCGATCGCAAGACCGCCGATGCCCATAAACGCATCCTTCTGGCTTGTCTCGTCACAATAATATAAGA
TGCGTGGACGCGGCTCTTGCAAAGTGGGGTCTAGCAATGCTGGAAAAGACGCGTACGGAGGCGTACCGCCATGGGCTCAGGAAAATAATCGAGAAAAGCC
CCAAGGTGCAATCAGGAAATGCGCGGGCACCCGCAGGGGCGTGTGGCGACAAGCAAGCGGCGGAAACCCCTAGCACACAAATCGCGCGGAGATCTGACGC
TTCTCGCGAACGCTGACGCGAAGGAACATGCTGCGCGGCATGTATCAACTCCGAAGCGGAGGGCCTGGAGCCTCTTGGTTGATGGCGCCGCTTAGGCATC
TTCCGTGGACGGCGGCGGAAGCGCCTCGGCTGGCTTCACTCCACGTGCCAGCCGGTCAAGGAACAGGGGCGCGCTCGCACCTAGATAAAATGATGTGAGT
GCGTTGGGCGCCTCGGCTATGGCAGGGATAGTGCCGCTAGCCAGTGCTAAGAGCGCCCGCACACAAAGGTAGAATGGCTGTTTGTAGCGCTCAGGGCAGC
GCCCATTCAGATCGCTGCACTCCTTGAACGCCGCGCCTAGTTCGACAGCGACACTTCCAATGACGCCGAGCCAGTAGGGAGATAGCTCGCCTACGAACGG
AAGGAGAGCCACTTAGGCGTGCTTAGAACGCTCGCGCTGACTCCGAGTGCTCCCGCCCAGCCGCAGATAGGAAGCGAGAGGGCTGAGACGATGCACGACG
ATGCGCGCCTGGGTGGGCCGGTGCGACAGCCGCAAGGCGGCAACCACGACGTTGGCGGCAAAAAGCAGTACGCCCACGCCGACGAGGGCTGTGAACACAA
TTTGCGTCACGAGTTCCATCCTGTCCCTCCAGCTTGGCTGTAAGCGGAGGATGATGCTTTTTGTCTTTCTAGGATGTGAATAACTGGCACTGCCCCCTTG
CGGCGTCAATGCCGCGTGCCGCCGGATTTTTGCAGGCCGATGTGATGAGGCGTACGGTTGTCCGTTTTTGAAAGGCCGAACTGATGCCGACAGACACGCC
GACCAAGCCCAAACCTTCCAAGCTCGGCGCCGAGAAGCTGACGCCAGAGGAACAGAAGCGTCGCTTCATTGAAGCCGCGCGGGAAGCGGGTGTGAGCGAG
AACGAAGCGGACTTCGATGCGGCGTTGAAGCAGATCGCGAAGCCGAAGCAGCCCAACTCCAGCCAAGGGTAGAGTGTGGCACTCTCACGCTAGCCCACGT
CGCGCAGTCGCGTTAGCAGCGCCGTAGAAAGGGGCTGCGAAATGTTAACGAACTCGATCATCGGCATGATACGCCGCCGCTGGCGGACCGAGCGTCCTTT
CCAAGGGCACCCGAAGGAAAGCTTGCTGCGGAAGATCCAGAACGATGCGGTTCGCGAGGACGTCAAGGAGCACGCGCGTCGCGAGCTACAGCTTCGCAAA
GATCAGCGCCTAATCGGCTGAGCTGAGCACTGCCGCCATTTGTCGATGCAGGTCGTCGGCAACGGACTGGGGAAGCAGCACGACCGCCTCGGTTCCGTCC
GCCTTCTCCAAGCGAACGCCCAACGTCCGGCCGCCGTCGATCAACTCGACGCTGATAACCCGACCAACCGCAAACGCGTCGGGGGCGTCATCACTCAACA
TGCACTGAACTCCACGAAGGGCGAGACTCGCCCAACGGTCGTGGTCGGTCAAGGTGTATGTGTGAACGATATAATCGCC

Protein section

ORF number : 5

ORF 1

Length		Begin	End	Strand	Fusion ORF
783 bp	260 aa	202	984	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGLRRCGDCKKQFTVTVGTVFESSHVKLHLWLQAAHLLASSKKGFSAHQLHRTLGVTYKTAWFMFHRLREAMRDGALAPMGGLDGPAGGAGIVEADETFI
GREPGKPKKRAYHHKMKVLSLVDRDTKQVRSVVVDDLKPDTVKPILAANIAKEASLFTDEAGHYVKLGKDFIEHQITSHGKGEYVRGIVHTNTVEGYFSV
FKRGMKGVYQHCGKQHLHRYLAEFDFRYNNRVKLGVGDVERAERALRGVVGKRLTYQTTR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
540 bp	179 aa	1606	1067	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

LLFKLIETNIVNFHIRFQEMGKYDHSISGERKLVDTVSKAHYQLMLHRAVRYYGAEYDIHIRPDNGNCTSLLPGYKQKLNEGAVSEFNHPPNCVRTIEPR
DSKQTPFLQFLDVTLGALTAYRNGRHLLPETSDMKRKLAIYAFEKTKLHSLEASTSISQHRLSVWNVRPKFNLKRGPRA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
186 bp	61 aa	2598	2413	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

VFTALVGVGVLLFAANVVVAALRLSHRPTQARIVVHRLSPLASYLRLGGSTRSQRERSKHA

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
189 bp	62 aa	2784	2972	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPTDTPTKPKPSKLGAEKLTPEEQKRRFIEAAREAGVSENEADFDAALKQIAKPKQPNSSQG

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
240 bp	80 aa	3240	3479	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

LSMQVVGNGLGKQHDRLGSVRLLQANAQRPAAVDQLDADNPTNRKRVGGVITQHALNSTKGETRPTVVVGQGVCVNDIIA

Blast result :

Comments

ISMdi18 is 98% aa similar to ISMpo2.
The first ORF is the transposase, the others are passengers genes annotated as hypothetical protein.

References

1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.