ISPar2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007204 | ND | Psychrobacter arcticus | Psychrobacter arcticus 273-4 |
DNA section
IS Length : 1189 bp
Ends
IR Length : 15/20
IRL : GGTAATCCCCCCCCCAATAAATAAGAACACTTAGAAATAGAGATTAACTG
IRR : GGTAATCATCCCAACGATAAGATGCTAATCAAATAGAATTAAGCTGCCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTTATTAAA | CAGACACCAA | 0 |
DNA sequence
GGTAATCCCCCCCCCAATAAATAAGAACACTTAGAAATAGAGATTAACTGCTAAAATATACCAGCAGGAGAATCCTATGAAAAAGATACGCTTTACTGAC
AACCAAATCGTCAACATCCTCAAACAAGCAGAACAGGGTGTGCCTATCGCTGATTTATGCCGTGAGCATAATATCGGCCAAAGCACGTTCTACAACTGGC
GTTCTAAATATGGTGGTATGGATGCCGCGCTTATCAGTCGCTTAAAAGAGCTTGAAGTTGAGAATGCTCGATTAAAGAAGATGTATGCTGATGAATGTCT
TAAATCAGACATACTACAGGATGCCATGTCAAAAAAGTGGTAGCGCCGCTTGGGCTTGTCACTACATCAAAGAGCGTAGGATCAGCATCCGTAGGGCTTG
CTTGATCTTTAATATCAGCGTGACCTGCTATTATCATAAGTCTGCGGCTTCTGACGAGAATAAGCAGATAGCTGACTTGCTTGTAGAACTGACTACCCAG
AATAAGAACTGGGGCTTTGGGTTGTGCTTTTTAAGTCTGCGTAACGTGTTGGGACTGCCATACAACCATAAGCGTGTGTATCGCATCTACTGCGAGCTTG
AATTAAACCTTAGAATCAAACCTAAGCGCCGTATTAAACGCGTTAAACCCGTACCACTTGCCGTACCTGTAGAACCGAATCAAAGCTGGAGCATGGACTT
CATGCATGATGCTTTAACCGATGGCAGGGCATTTAGACTGTTTAATGTCATTGATGATTACAACCGTGAAGCCTTAACGGTTGAGATTGACTTCTCACTG
CCCGCCCAAAGGGTTATACGCAGCTTAAATCAGTTAATTGAATACCGAGGTAAACCGGTTCAAGTAAGATGTGATAATGGTGCCGAGTACATTAGCAATG
CGCTCAAAGACTGGGCGGTAAATCAAGGTATTACGATAAGATATATTGAGCCTGGCAATCCACAACAAAACGCTTATGTTGAGCGCTATAATAGAACGAT
GCGTTATGATTGGTTAAACCAAGAGCTGTTTACTGATTTGGATCAAGTCCGTCAGCAAGCAGAGGACTGGTTATATCACTACAATAACGAGCGCCCTAAT
ATGGGTAATGGCGGTTTTACACCGATACAGAAACTACATCAGGCAGCTTAATTCTATTTGATTAGCATCTTATCGTTGGGATGATTACC
AACCAAATCGTCAACATCCTCAAACAAGCAGAACAGGGTGTGCCTATCGCTGATTTATGCCGTGAGCATAATATCGGCCAAAGCACGTTCTACAACTGGC
GTTCTAAATATGGTGGTATGGATGCCGCGCTTATCAGTCGCTTAAAAGAGCTTGAAGTTGAGAATGCTCGATTAAAGAAGATGTATGCTGATGAATGTCT
TAAATCAGACATACTACAGGATGCCATGTCAAAAAAGTGGTAGCGCCGCTTGGGCTTGTCACTACATCAAAGAGCGTAGGATCAGCATCCGTAGGGCTTG
CTTGATCTTTAATATCAGCGTGACCTGCTATTATCATAAGTCTGCGGCTTCTGACGAGAATAAGCAGATAGCTGACTTGCTTGTAGAACTGACTACCCAG
AATAAGAACTGGGGCTTTGGGTTGTGCTTTTTAAGTCTGCGTAACGTGTTGGGACTGCCATACAACCATAAGCGTGTGTATCGCATCTACTGCGAGCTTG
AATTAAACCTTAGAATCAAACCTAAGCGCCGTATTAAACGCGTTAAACCCGTACCACTTGCCGTACCTGTAGAACCGAATCAAAGCTGGAGCATGGACTT
CATGCATGATGCTTTAACCGATGGCAGGGCATTTAGACTGTTTAATGTCATTGATGATTACAACCGTGAAGCCTTAACGGTTGAGATTGACTTCTCACTG
CCCGCCCAAAGGGTTATACGCAGCTTAAATCAGTTAATTGAATACCGAGGTAAACCGGTTCAAGTAAGATGTGATAATGGTGCCGAGTACATTAGCAATG
CGCTCAAAGACTGGGCGGTAAATCAAGGTATTACGATAAGATATATTGAGCCTGGCAATCCACAACAAAACGCTTATGTTGAGCGCTATAATAGAACGAT
GCGTTATGATTGGTTAAACCAAGAGCTGTTTACTGATTTGGATCAAGTCCGTCAGCAAGCAGAGGACTGGTTATATCACTACAATAACGAGCGCCCTAAT
ATGGGTAATGGCGGTTTTACACCGATACAGAAACTACATCAGGCAGCTTAATTCTATTTGATTAGCATCTTATCGTTGGGATGATTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 77 | 343 | + | No |
Description : First part of the transposase
ORF sequence :
MKKIRFTDNQIVNILKQAEQGVPIADLCREHNIGQSTFYNWRSKYGGMDAALISRLKELEVENARLKKMYADECLKSDILQDAMSKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 876 bp | 291 aa | 276 | 1151 | + | No |
Description : Second part of the transposase
ORF sequence :
RRCMLMNVLNQTYYRMPCQKSGSAAWACHYIKERRISIRRACLIFNISVTCYYHKSAASDENKQIADLLVELTTQNKNWGFGLCFLSLRNVLGLPYNHKR
VYRIYCELELNLRIKPKRRIKRVKPVPLAVPVEPNQSWSMDFMHDALTDGRAFRLFNVIDDYNREALTVEIDFSLPAQRVIRSLNQLIEYRGKPVQVRCD
NGAEYISNALKDWAVNQGITIRYIEPGNPQQNAYVERYNRTMRYDWLNQELFTDLDQVRQQAEDWLYHYNNERPNMGNGGFTPIQKLHQAA
VYRIYCELELNLRIKPKRRIKRVKPVPLAVPVEPNQSWSMDFMHDALTDGRAFRLFNVIDDYNREALTVEIDFSLPAQRVIRSLNQLIEYRGKPVQVRCD
NGAEYISNALKDWAVNQGITIRYIEPGNPQQNAYVERYNRTMRYDWLNQELFTDLDQVRQQAEDWLYHYNNERPNMGNGGFTPIQKLHQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1075 bp | 357 aa | 77 | 1151 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKIRFTDNQIVNILKQAEQGVPIADLCREHNIGQSTFYNWRSKYGGMDAALISRLKELEVENARLKKMYADECLKSDILQDAMSKSGSAAWACHYIKER
RISIRRACLIFNISVTCYYHKSAASDENKQIADLLVELTTQNKNWGFGLCFLSLRNVLGLPYNHKRVYRIYCELELNLRIKPKRRIKRVKPVPLAVPVEP
NQSWSMDFMHDALTDGRAFRLFNVIDDYNREALTVEIDFSLPAQRVIRSLNQLIEYRGKPVQVRCDNGAEYISNALKDWAVNQGITIRYIEPGNPQQNAY
VERYNRTMRYDWLNQELFTDLDQVRQQAEDWLYHYNNERPNMGNGGFTPIQKLHQAA
RISIRRACLIFNISVTCYYHKSAASDENKQIADLLVELTTQNKNWGFGLCFLSLRNVLGLPYNHKRVYRIYCELELNLRIKPKRRIKRVKPVPLAVPVEP
NQSWSMDFMHDALTDGRAFRLFNVIDDYNREALTVEIDFSLPAQRVIRSLNQLIEYRGKPVQVRCDNGAEYISNALKDWAVNQGITIRYIEPGNPQQNAY
VERYNRTMRYDWLNQELFTDLDQVRQQAEDWLYHYNNERPNMGNGGFTPIQKLHQAA
Blast result :
Comments
ISPar2 is 76% aa similar to ISKpn10.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
This IS has atypic IRs: it begins with 'GG' instead of 'TG'.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
This IS has atypic IRs: it begins with 'GG' instead of 'TG'.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Chain,P., Di Bartolo,G., Ivanova,N., Hauser,L., Land,M., Larimer,F., Pitluck,S. and Richardson,P. (2005) Direct submission GenBank.
2] ISfinder annotation (2009).
2] ISfinder annotation (2009).