ISMex11

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
Methylobacterium dichloromethanicum DM4
DNA section
IS Length : 1227 bp

Ends


IR Length : 22/32

IRL : TGACGTCCCCCCATCGCTTTGGTCCGGCGTGAGTGCGAGTTTTCCGGTTG
IRR : TGAGGGTCCCCCGGATTTTGGTCCGGCCGGAAGGTGAGCTTCCGGATCTC

Insertion site


Left flankDirect repeatRight flankDR Length
GTGGAGCAAAGGCCGACCCTGCAC4
CCAAGCTGTCACAGCCCGAGAACT4
GCATCCACCGGCCACAGCGCCG2
CCAGGCGAGCCGCCGTCTGTAC2
TTTTTCACCCGCCGACCCTG0

DNA sequence

TGACGTCCCCCCATCGCTTTGGTCCGGCGTGAGTGCGAGTTTTCCGGTTGTGTGAGCCCCTGAGGGGAGGAGCAGAGCCGTGAAGAAGAGCAGGTTCACC
GAAGAGCAGATCGCCTTCGCGCTGAAGCAGGCCGAGACCGGGACACCGGTCGCGGAGGTGCTGCGCCGGATGGGCATCTCCGAACAGACGTTTTACCGCT
GGAAGAAGCTCTACGGCGGCTTGGGCACAGGCGAGCTGCGGCGGCTGAAGCAGCTTGAGGACGAGAACCGCAAGCTCAAGCAGCTGGTTGCCGATCTGAG
CCTCGACAAGCACATCCTGCAGGACGTGCTCGCAAAAAAGCTCTGACGCCTGCTCGGCGACGCGAGCTCGTGCACCAGGTTCAGGAGGCGCACGGGGTGA
GCGAGCGGCGCGGCTGCGCCGCGCTCGGCGTCGGTCGGTCGGGCGTCCGCTACCGCTCGACCAAGCCCGATCAGGCACCGCTGCGGATGCGCATCTGTGA
TCTGGCCAAGAGCCGGGTGCGCTACGGGTACTTCCGGATCTACATCCTGCTGCGCCGGGAAGGTTGGCGGGTGAATCACAAGAGGGTCCACCGCCTCTAC
AGGGATGAGGGCCTCAGCCTGCGGCTCAAGCGGCCGCGCCGGCACGTCAGCGCGGCACACCGTGAGCGTCAGCCTGCTGCGCTCCGGCCGAACGAACGCT
GGTCGATGGACTTCGTCTCCGACGCGCTGTTCGACGGCCGCCGGCTGCGGGCCCTGACGGTCGTCGATGCATTCACGCGCGAGGCTCTGGCGATCGAGGT
CGACCAGGGCATCAAAGGCGAACAGGTCGTGGCTGTCGTCGGCCGCTTGGCGCTGCTGCGCGGAGCACCATGCGCGATTCAGGTCGATAACGGGCCTGAG
TTCGTCTCGAAAGCCCTCGACCGCTGGGCCTACGAGAACGGTGTCACGTTGGACTTCTCACGGCCCGGCAAGCCGACCGACAACGCGCTGGTCGAGTCGT
TCAACGGCCGGCTGCGCGACGAGTGCCTGAACGCGAACTGGTTCTTGTCGTTGGCCGACGCGAGGAGCAAGATCGAGACGTGGCGGCGGCACTACAACGA
GAGCCGTCCTCACACCGCCCTGGGGTGGCGAACACCCCAGGAATTCGCCCTAGCGGCGGCCCTGCAGGCCGCCGAATGAGATCCGGAAGCTCACCTTCCG
GCCGGACCAAAATCCGGGGGACCCTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa80346+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

VKKSRFTEEQIAFALKQAETGTPVAEVLRRMGISEQTFYRWKKLYGGLGTGELRRLKQLEDENRKLKQLVADLSLDKHILQDVLAKKL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
921 bp306 aa2591179+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GREPQAQAAGCRSEPRQAHPAGRARKKALTPARRRELVHQVQEAHGVSERRGCAALGVGRSGVRYRSTKPDQAPLRMRICDLAKSRVRYGYFRIYILLRR
EGWRVNHKRVHRLYRDEGLSLRLKRPRRHVSAAHRERQPAALRPNERWSMDFVSDALFDGRRLRALTVVDAFTREALAIEVDQGIKGEQVVAVVGRLALL
RGAPCAIQVDNGPEFVSKALDRWAYENGVTLDFSRPGKPTDNALVESFNGRLRDECLNANWFLSLADARSKIETWRRHYNESRPHTALGWRTPQEFALAA
ALQAAE

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1100 bp366 aa801179+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VKKSRFTEEQIAFALKQAETGTPVAEVLRRMGISEQTFYRWKKLYGGLGTGELRRLKQLEDENRKLKQLVADLSLDKHILQDVLAKKALTPARRRELVHQ
VQEAHGVSERRGCAALGVGRSGVRYRSTKPDQAPLRMRICDLAKSRVRYGYFRIYILLRREGWRVNHKRVHRLYRDEGLSLRLKRPRRHVSAAHRERQPA
ALRPNERWSMDFVSDALFDGRRLRALTVVDAFTREALAIEVDQGIKGEQVVAVVGRLALLRGAPCAIQVDNGPEFVSKALDRWAYENGVTLDFSRPGKPT
DNALVESFNGRLRDECLNANWFLSLADARSKIETWRRHYNESRPHTALGWRTPQEFALAAALQAAE

 

Blast result :
Comments
ISMex11 is 78% aa similar to ISBcen15.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References