ISMdi10
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1186 bp
Ends
IR Length : 13/15
IRL : gcgctgaTCGCGACCCGGCGGGCGGCGGTGCCACACTGGGGTGCGATGCG
IRR : ----gcgTCACGTCCCGGCGGGGACGCTTGCGTCCGCCTATGGCGGAACT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACCGTTCAACAT | AGGCCATCCCGA | 0 |
DNA sequence
GCGCTGATCGCGACCCGGCGGGCGGCGGTGCCACACTGGGGTGCGATGCGGGCGGCGGATCCGCCCGCTGTCACCTCGGGAGGCTGCTATGGCGCACTAC
GCCGCGTTCGACGTCTCAGACAAGGAGACCGCTATCCATGTGCTCGACGAGCACGGCAAGCTGGTGTGGAAGGGCAAGCGGCCGAGCGAGCCGGAAGCCT
TAGCCGAAGCGCTGCGGCGTCATGCCCCCGAACTGGTGCGCGTTGGCCTGGAGACGGGCCGGCTCGCGCCGTGGCTCTACCACTCGCTCAAGGAGCTGCG
TGTGCCCGTCATCTGCCTCGATGCCCGGCATGCCCGGGCTGCGACGGCCTTGCAGCGGAACAAGACCGATGCGCGCGATGCCGAGACGCTGGCGCAGCTC
GTGCGCGTAGGCTGGTACCGGGAAGCACGCGTCAAAGGCTGGGCCGCGCACGCCGTGCGGCATCTGATCGGCGCTCGCGCCCAGCTTGTCGGCATGGCAA
CTGATCTGTCGAACCAGCTCCGGAGCCTGCTCAGGACCTTCGGCCGGCGCGTGACAGGCCGGGCCGGAGGCGCCTTCGAGGCGAGGGTGCGCGAGCAGAT
CAAGGACAAGCCCGAGATGGATGCCGTGGCCGAGCCACTGCTGACGGCGTGGCGGGCGATACGCGACCAGATTGCCGTTCTCGACCGCAGGCTGATCGAA
GCCGCCAGAGACGATGCGACCTGCCGGCTGCTAATGTCCTGCCCGGGCGTGGGTGTCGTCGTGGCGGCGAGCTTCGCGGCGGCCGTGGAGGTGCCGGAGC
ACTTCCGGCGCTCGCGCTCGGTGGGGGCGTATCTCGGGCTGACGCCCAGGCGCCGTCAGTCGGGGGAGAGCGACCACAGCACAGGCGTGTCGAAGCGAGG
AGACAAGCTGCTGCGCAGCTACCTGTTCGAGGCAGCAGCCAGTTTGCTGATCCGCGTCCAGCGGCCATCGGCGCTCAAGACCTGGGGCGTTGGGCTCGTG
CAGCGCCTCGGATTCAAGCGGGCGGCGGTGGCTGTCGCGCGCAAGCTTGGCGTCGTGTTGCACGCCATGTGGCGGGCGAACAGACCGTTCGAGGCGTGGC
CGAGCGCCGTCGCTGCGGCGGCCTGAGCCGCCTGACCCGCAGTTCCGCCATAGGCGGACGCAAGCGTCCCCGCCGGGACGTGACGC
GCCGCGTTCGACGTCTCAGACAAGGAGACCGCTATCCATGTGCTCGACGAGCACGGCAAGCTGGTGTGGAAGGGCAAGCGGCCGAGCGAGCCGGAAGCCT
TAGCCGAAGCGCTGCGGCGTCATGCCCCCGAACTGGTGCGCGTTGGCCTGGAGACGGGCCGGCTCGCGCCGTGGCTCTACCACTCGCTCAAGGAGCTGCG
TGTGCCCGTCATCTGCCTCGATGCCCGGCATGCCCGGGCTGCGACGGCCTTGCAGCGGAACAAGACCGATGCGCGCGATGCCGAGACGCTGGCGCAGCTC
GTGCGCGTAGGCTGGTACCGGGAAGCACGCGTCAAAGGCTGGGCCGCGCACGCCGTGCGGCATCTGATCGGCGCTCGCGCCCAGCTTGTCGGCATGGCAA
CTGATCTGTCGAACCAGCTCCGGAGCCTGCTCAGGACCTTCGGCCGGCGCGTGACAGGCCGGGCCGGAGGCGCCTTCGAGGCGAGGGTGCGCGAGCAGAT
CAAGGACAAGCCCGAGATGGATGCCGTGGCCGAGCCACTGCTGACGGCGTGGCGGGCGATACGCGACCAGATTGCCGTTCTCGACCGCAGGCTGATCGAA
GCCGCCAGAGACGATGCGACCTGCCGGCTGCTAATGTCCTGCCCGGGCGTGGGTGTCGTCGTGGCGGCGAGCTTCGCGGCGGCCGTGGAGGTGCCGGAGC
ACTTCCGGCGCTCGCGCTCGGTGGGGGCGTATCTCGGGCTGACGCCCAGGCGCCGTCAGTCGGGGGAGAGCGACCACAGCACAGGCGTGTCGAAGCGAGG
AGACAAGCTGCTGCGCAGCTACCTGTTCGAGGCAGCAGCCAGTTTGCTGATCCGCGTCCAGCGGCCATCGGCGCTCAAGACCTGGGGCGTTGGGCTCGTG
CAGCGCCTCGGATTCAAGCGGGCGGCGGTGGCTGTCGCGCGCAAGCTTGGCGTCGTGTTGCACGCCATGTGGCGGGCGAACAGACCGTTCGAGGCGTGGC
CGAGCGCCGTCGCTGCGGCGGCCTGAGCCGCCTGACCCGCAGTTCCGCCATAGGCGGACGCAAGCGTCCCCGCCGGGACGTGACGC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1086 bp | 361 aa | 41 | 1126 | + | No |
Chemistry : DEDD
ORF sequence :
VRCGRRIRPLSPREAAMAHYAAFDVSDKETAIHVLDEHGKLVWKGKRPSEPEALAEALRRHAPELVRVGLETGRLAPWLYHSLKELRVPVICLDARHARA
ATALQRNKTDARDAETLAQLVRVGWYREARVKGWAAHAVRHLIGARAQLVGMATDLSNQLRSLLRTFGRRVTGRAGGAFEARVREQIKDKPEMDAVAEPL
LTAWRAIRDQIAVLDRRLIEAARDDATCRLLMSCPGVGVVVAASFAAAVEVPEHFRRSRSVGAYLGLTPRRRQSGESDHSTGVSKRGDKLLRSYLFEAAA
SLLIRVQRPSALKTWGVGLVQRLGFKRAAVAVARKLGVVLHAMWRANRPFEAWPSAVAAAA
ATALQRNKTDARDAETLAQLVRVGWYREARVKGWAAHAVRHLIGARAQLVGMATDLSNQLRSLLRTFGRRVTGRAGGAFEARVREQIKDKPEMDAVAEPL
LTAWRAIRDQIAVLDRRLIEAARDDATCRLLMSCPGVGVVVAASFAAAVEVPEHFRRSRSVGAYLGLTPRRRQSGESDHSTGVSKRGDKLLRSYLFEAAA
SLLIRVQRPSALKTWGVGLVQRLGFKRAAVAVARKLGVVLHAMWRANRPFEAWPSAVAAAA
Blast result :
Comments
ISMdi10 is 59% aa similar to ISMex9.
The IR of this IS are not at its termini. In the IS sequence as given7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end.
The IR of this IS are not at its termini. In the IS sequence as given7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.