ISMdi24
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 849 bp
Ends
IR Length : 13/17
IRL : GAGCAGGGGACAAAACCGGAGTTTGACGGGTTGAGCAGGTATGGATGCTC
IRR : GAACTAAGGACAAAACCTTTTCGCCTGTTTGCGGCAGATGAGGGCACAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGGTCGATCTTA | TAATTGTGCAGGG | 0 |
DNA sequence
GAGCAGGGGACAAAACCGGAGTTTGACGGGTTGAGCAGGTATGGATGCTCTCGTGCCCGACGATCTCTGGGCGGTGATCTCGCCGCTCCTTCCGCCTGAA
CCGGAGAAGCTGAAGGGCGGTCGTCCGCGTGCCTCGGATCGCTCTGCGCTGGCCGGCATCATCTTGGTTCTGCGCACCGGCATGCAGTGGAAGCACGTGC
CCGGGAACGCCCTGGGCTGCAGCGGCAAGACGAGTTGGCGGCGGCTCGGTGAATGGCAGGCGGCGGGTGTGTGGGCGAACCTGCATCGCGTGCTGCTTGA
ACAGCTTCAGGACGCGAACGCCTTGAACTGGTCACGGGCCGCGCTCGACAGCGCCAGCGTGCCAGCCAAAAAGGGGGCGCGGCGACCGGCCCGAACCCAA
CTGACCGCGGCAAGCCCGGCACCAAGCGTCACCTCGTCGTTGACGCCGATGGGACACCACTCGGCCTGACGCTGACGGGGGCCAACTGCCACGATAGCCG
CATGCTGGCTGCAACCCTCGACGCGGTACCGGGCCTACGGACAGGCCGCCGCGGTCGCCCGCGCCGGAGGCCGGACAAACTGCACGCCGACAAGGCCTAC
GATCATCGCCGCTGCCGGCAGGAATGCCGGGCGCGGAGCATCATGCCGCGCATCGCCCGCCGCGGTGTCGAGAGCAGCCGCCACCTCGGACGACATCGCT
GGATCGTTGAGCGTACTCTGGCGTGGATCGCCCGTTTCCGTCGCCTGGCGATCCGCTACGAGCGACGTGCCGACCTTCATCTCGCCTTCACGACACTCGC
TTGTGCCCTCATCTGCCGCAAACAGGCGAAAAGGTTTTGTCCTTAGTTC
CCGGAGAAGCTGAAGGGCGGTCGTCCGCGTGCCTCGGATCGCTCTGCGCTGGCCGGCATCATCTTGGTTCTGCGCACCGGCATGCAGTGGAAGCACGTGC
CCGGGAACGCCCTGGGCTGCAGCGGCAAGACGAGTTGGCGGCGGCTCGGTGAATGGCAGGCGGCGGGTGTGTGGGCGAACCTGCATCGCGTGCTGCTTGA
ACAGCTTCAGGACGCGAACGCCTTGAACTGGTCACGGGCCGCGCTCGACAGCGCCAGCGTGCCAGCCAAAAAGGGGGCGCGGCGACCGGCCCGAACCCAA
CTGACCGCGGCAAGCCCGGCACCAAGCGTCACCTCGTCGTTGACGCCGATGGGACACCACTCGGCCTGACGCTGACGGGGGCCAACTGCCACGATAGCCG
CATGCTGGCTGCAACCCTCGACGCGGTACCGGGCCTACGGACAGGCCGCCGCGGTCGCCCGCGCCGGAGGCCGGACAAACTGCACGCCGACAAGGCCTAC
GATCATCGCCGCTGCCGGCAGGAATGCCGGGCGCGGAGCATCATGCCGCGCATCGCCCGCCGCGGTGTCGAGAGCAGCCGCCACCTCGGACGACATCGCT
GGATCGTTGAGCGTACTCTGGCGTGGATCGCCCGTTTCCGTCGCCTGGCGATCCGCTACGAGCGACGTGCCGACCTTCATCTCGCCTTCACGACACTCGC
TTGTGCCCTCATCTGCCGCAAACAGGCGAAAAGGTTTTGTCCTTAGTTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 429 bp | 142 aa | 41 | 469 | + | No |
Description : First part of the transposase
ORF sequence :
MDALVPDDLWAVISPLLPPEPEKLKGGRPRASDRSALAGIILVLRTGMQWKHVPGNALGCSGKTSWRRLGEWQAAGVWANLHRVLLEQLQDANALNWSRA
ALDSASVPAKKGARRPARTQLTAASPAPSVTSSLTPMGHHSA
ALDSASVPAKKGARRPARTQLTAASPAPSVTSSLTPMGHHSA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 546 bp | 181 aa | 301 | 846 | + | No |
Description : Second part of the transposase
ORF sequence :
TASGRERLELVTGRARQRQRASQKGGAATGPNPTDRGKPGTKRHLVVDADGTPLGLTLTGANCHDSRMLAATLDAVPGLRTGRRGRPRRRPDKLHADKAY
DHRRCRQECRARSIMPRIARRGVESSRHLGRHRWIVERTLAWIARFRRLAIRYERRADLHLAFTTLACALICRKQAKRFCP
DHRRCRQECRARSIMPRIARRGVESSRHLGRHRWIVERTLAWIARFRRLAIRYERRADLHLAFTTLACALICRKQAKRFCP
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 806 bp | 266 aa | 41 | 846 | + | Yes |
Chemistry : DDE
ORF sequence :
MDALVPDDLWAVISPLLPPEPEKLKGGRPRASDRSALAGIILVLRTGMQWKHVPGNALGCSGKTSWRRLGEWQAAGVWANLHRVLLEQLQDANALNWSRA
ALDSASVPAKKAATGPNPTDRGKPGTKRHLVVDADGTPLGLTLTGANCHDSRMLAATLDAVPGLRTGRRGRPRRRPDKLHADKAYDHRRCRQECRARSIM
PRIARRGVESSRHLGRHRWIVERTLAWIARFRRLAIRYERRADLHLAFTTLACALICRKQAKRFCP
ALDSASVPAKKAATGPNPTDRGKPGTKRHLVVDADGTPLGLTLTGANCHDSRMLAATLDAVPGLRTGRRGRPRRRPDKLHADKAYDHRRCRQECRARSIM
PRIARRGVESSRHLGRHRWIVERTLAWIARFRRLAIRYERRADLHLAFTTLACALICRKQAKRFCP
Blast result :
Comments
ISMdi24 is 93% aa similar to ISMex19. The third ORF is a putative oRFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009)PLoS ONE Submitted.