ISMdi5
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium extorquens AM1 Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1279 bp
Ends
IR Length : 24/26
IRL : TGATCCTCCCTCGGCATCGCGGACACCTCTGATACGCTCATGCTGAGCGG
IRR : TGATCCTCCCTCGGTGTCGCGGACACAGGGACATCATTTCGCGGTCCTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTATCGTCTG | GGGTGCCCTC | 0 |
DNA sequence
TGATCCTCCCTCGGCATCGCGGACACCTCTGATACGCTCATGCTGAGCGGGGAAGGTGTCGGATGGCGAAGACGAGACGAGAGTTCACACCCGAGTTCAA
ACGCGAAGCGGTCGCCCTGCTGGAGAGCAGTGGCCGGCCGCAGATGCAGGTCGCGGCCGAGCTTGGGATCCAGCCCTCGATGCTGAGGCAGTGGCGTGCC
ACGCTGAACGGGGCCTCACCCGGGCCGCGGTCTGCTGGATCGACGGTCGCCTCGCCACCAAGCCCGGTTGCGTCCCCGTCCGATCAGGCCGCCGAGATCG
CCCGACTGCGGCGGGAGCTCGACCGCACGCGCATGGAGCGCGACGTACTAAAAAAAGCCATCGGCATCTTCGCGGAGATGCCCAAGTGACCTTCGCCTTC
ATCGAGCAGCATGCGCGGACCTGGCCGGTGCGTCTCATGTGCCGCGTGCTCGGAGTCTCACCCAGCGGCTTCTACGGATGGCGGTCACGGCCTGAGAGCG
CCCGCTCGGCCTCCAATCGCCAACTCCTGGACGACGTCCGGCGCATCCATGCCGCTCACCACCGACGCTATGGTGCCCCACGCGTGCATGCCGCCCTGCG
AGCCGAGGGCCGATCCGTCAGCCGCGGGCGGGTGGAGCGTCTCATGCGCCGGCATGGCATCCGCGCCCTGGCGGGGCGTCGGTTCCGGCCCTGTACGACC
GACAGCCGTCACGATCTCCCCATCGCGCCCAATCTCCTCAAGCAGGACTTCTCAGCTGCGCGACCCGACACGGTCTGGCTGGCCGATATCACCTACCTGC
CGACCGGCGAGGGCTGGCTGTACTTGGCCGCCGTCCTCGATCTGGCCACGCGCAAGATCGTCGGTTGGTCGATGCGCGATCACATGCGCGCCGAGCTGAG
CGTCGCGGCACTGATGATGGCCGCCCAGCGGCAGCGGCCGGCCGCCGGGCTCATCTGCCACTCGGATCGCGGCAGCCAGTACGCGGCCGAGGCCTACCGG
AGACAGCTCTCCAGTATGGGGGCGAGGCCGTCGATGAGCCGCACGGGTTGCTGCTACGACAACGCCCCGATGGAGAGCTTCTTCCACACGCTCAAGGTCG
AGCTCGTCCATCAGCGACGATGGGCGACACGGGATGAAGCGCGGCGCGATCTGTTCGCCTACATCGAGGGCTACTACAATCGGCAACGCATCCACTCGGC
ACTCGGCTATCTCACGCCCGAACAAGCCGAGAGGACCGCGAAATGATGTCCCTGTGTCCGCGACACCGAGGGAGGATCA
ACGCGAAGCGGTCGCCCTGCTGGAGAGCAGTGGCCGGCCGCAGATGCAGGTCGCGGCCGAGCTTGGGATCCAGCCCTCGATGCTGAGGCAGTGGCGTGCC
ACGCTGAACGGGGCCTCACCCGGGCCGCGGTCTGCTGGATCGACGGTCGCCTCGCCACCAAGCCCGGTTGCGTCCCCGTCCGATCAGGCCGCCGAGATCG
CCCGACTGCGGCGGGAGCTCGACCGCACGCGCATGGAGCGCGACGTACTAAAAAAAGCCATCGGCATCTTCGCGGAGATGCCCAAGTGACCTTCGCCTTC
ATCGAGCAGCATGCGCGGACCTGGCCGGTGCGTCTCATGTGCCGCGTGCTCGGAGTCTCACCCAGCGGCTTCTACGGATGGCGGTCACGGCCTGAGAGCG
CCCGCTCGGCCTCCAATCGCCAACTCCTGGACGACGTCCGGCGCATCCATGCCGCTCACCACCGACGCTATGGTGCCCCACGCGTGCATGCCGCCCTGCG
AGCCGAGGGCCGATCCGTCAGCCGCGGGCGGGTGGAGCGTCTCATGCGCCGGCATGGCATCCGCGCCCTGGCGGGGCGTCGGTTCCGGCCCTGTACGACC
GACAGCCGTCACGATCTCCCCATCGCGCCCAATCTCCTCAAGCAGGACTTCTCAGCTGCGCGACCCGACACGGTCTGGCTGGCCGATATCACCTACCTGC
CGACCGGCGAGGGCTGGCTGTACTTGGCCGCCGTCCTCGATCTGGCCACGCGCAAGATCGTCGGTTGGTCGATGCGCGATCACATGCGCGCCGAGCTGAG
CGTCGCGGCACTGATGATGGCCGCCCAGCGGCAGCGGCCGGCCGCCGGGCTCATCTGCCACTCGGATCGCGGCAGCCAGTACGCGGCCGAGGCCTACCGG
AGACAGCTCTCCAGTATGGGGGCGAGGCCGTCGATGAGCCGCACGGGTTGCTGCTACGACAACGCCCCGATGGAGAGCTTCTTCCACACGCTCAAGGTCG
AGCTCGTCCATCAGCGACGATGGGCGACACGGGATGAAGCGCGGCGCGATCTGTTCGCCTACATCGAGGGCTACTACAATCGGCAACGCATCCACTCGGC
ACTCGGCTATCTCACGCCCGAACAAGCCGAGAGGACCGCGAAATGATGTCCCTGTGTCCGCGACACCGAGGGAGGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 327 bp | 108 aa | 63 | 389 | + | No |
Description : First part of the transposase
ORF sequence :
MAKTRREFTPEFKREAVALLESSGRPQMQVAAELGIQPSMLRQWRATLNGASPGPRSAGSTVASPPSPVASPSDQAAEIARLRRELDRTRMERDVLKKAI
GIFAEMPK
GIFAEMPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1065 bp | 354 aa | 182 | 1246 | + | No |
Description : Second part of the transposase
ORF sequence :
VPVRSGRRDRPTAAGARPHAHGARRTKKSHRHLRGDAQVTFAFIEQHARTWPVRLMCRVLGVSPSGFYGWRSRPESARSASNRQLLDDVRRIHAAHHRRY
GAPRVHAALRAEGRSVSRGRVERLMRRHGIRALAGRRFRPCTTDSRHDLPIAPNLLKQDFSAARPDTVWLADITYLPTGEGWLYLAAVLDLATRKIVGWS
MRDHMRAELSVAALMMAAQRQRPAAGLICHSDRGSQYAAEAYRRQLSSMGARPSMSRTGCCYDNAPMESFFHTLKVELVHQRRWATRDEARRDLFAYIEG
YYNRQRIHSALGYLTPEQAERTAK
GAPRVHAALRAEGRSVSRGRVERLMRRHGIRALAGRRFRPCTTDSRHDLPIAPNLLKQDFSAARPDTVWLADITYLPTGEGWLYLAAVLDLATRKIVGWS
MRDHMRAELSVAALMMAAQRQRPAAGLICHSDRGSQYAAEAYRRQLSSMGARPSMSRTGCCYDNAPMESFFHTLKVELVHQRRWATRDEARRDLFAYIEG
YYNRQRIHSALGYLTPEQAERTAK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1184 bp | 394 aa | 63 | 1246 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKTRREFTPEFKREAVALLESSGRPQMQVAAELGIQPSMLRQWRATLNGASPGPRSAGSTVASPPSPVASPSDQAAEIARLRRELDRTRMERDVLKKSH
RHLRGDAQVTFAFIEQHARTWPVRLMCRVLGVSPSGFYGWRSRPESARSASNRQLLDDVRRIHAAHHRRYGAPRVHAALRAEGRSVSRGRVERLMRRHGI
RALAGRRFRPCTTDSRHDLPIAPNLLKQDFSAARPDTVWLADITYLPTGEGWLYLAAVLDLATRKIVGWSMRDHMRAELSVAALMMAAQRQRPAAGLICH
SDRGSQYAAEAYRRQLSSMGARPSMSRTGCCYDNAPMESFFHTLKVELVHQRRWATRDEARRDLFAYIEGYYNRQRIHSALGYLTPEQAERTAK
RHLRGDAQVTFAFIEQHARTWPVRLMCRVLGVSPSGFYGWRSRPESARSASNRQLLDDVRRIHAAHHRRYGAPRVHAALRAEGRSVSRGRVERLMRRHGI
RALAGRRFRPCTTDSRHDLPIAPNLLKQDFSAARPDTVWLADITYLPTGEGWLYLAAVLDLATRKIVGWSMRDHMRAELSVAALMMAAQRQRPAAGLICH
SDRGSQYAAEAYRRQLSSMGARPSMSRTGCCYDNAPMESFFHTLKVELVHQRRWATRDEARRDLFAYIEGYYNRQRIHSALGYLTPEQAERTAK
Blast result :
Comments
ISMdi5 has 1 intact and 1 partial copies in DM4 and 1 partial copy in AM1.
ISMdi5 is 74% aa similar to ISCausp1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISMdi5 is 74% aa similar to ISCausp1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References