ISMdi3
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1246 bp
Ends
IR Length : 32/40
IRL : TGACCTGACCGGCTGGCTTTGGACCGGGCTGATTGAGAGGATCAGCTAGG
IRR : TGACCTGACCGGTATTTTGGACCGGGCCAGTTGCGAGGCTACTGCATGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGCGAACTC | CGGCCGCCTC | 0 | |
| GGCGCCGGCT | AGACCTTCGG | 0 | |
| AGGTGGCTAG | GGCGGCCATG | 0 | |
| ATGTGCAGGT | CTGCCATGTG | 0 |
DNA sequence
TGACCTGACCGGCTGGCTTTGGACCGGGCTGATTGAGAGGATCAGCTAGGACCGAAGGAGTTGGACATGCGACGAGGACAGAAGACGAGCGCGGAGCAGG
TCGTTTTGAAGCTGCGCCAGATCGAGGTGCAGACCGCCCAGGGCAAGAGTTTGGCGTTGGCGTGCAAGGAGGCGGAGATCTCCGAGCAGAGCTATTACCG
CTGGCGCAAGGAGTTCGGCGGCCTGCAGGTCGATCAAGCCAGGAAGATGAAGGACCTAGAGCGCGAGAACGCTCGCTTGCGGCGGCTTGTGGCTGACCTA
TCCCTGGAAAAGCAGGTGTTGGCGGACGTCGCCTCGGGAAACTTGTAGCCCCCGAGCGACGCCGGCAGGCGGTCGACGGTATCCGAGCCAAGTACGGCCT
CTCGGAACGTCACGCCTGCCGGATCGTCGGCCAGCATCGGGGTACGCAACGCTACGTGCCGACCGTGCTGGCCGACGAGGATGGGCTGACCCGAGCCATC
GTTGCCCTGGCGTCCGAGTACGGACGCTATGGCTACCGCCGCGTCACCGCGCTGCTGCAGGCAGCCGGCTGGCAGGTGGGCAAGGATCGGGTTCAGCGCA
TCTGGCGTCGCGAGGGGCTGAAGGTCCCCAGCCGACATAGACCCCGCAGCCGCCTCTGGCTGAACGACGGCTCATGCGTGCGACTACGGCCGCTTCGCCG
CAATCACGTCTGGAGCTTCGACTTCGTGCAGGCTCAGACGCACGATGGGCGAAGCCTGCGCATCCTGACGTTGATCGATGAGCACAGCCGGGCGTGCCTC
GCGCTGAAGGTGGCGCGGCGCATCAACAGCCTCGGTGTGATCGAGGCGCTGGCCGACGCCATGTGCCTGCATGGCATCCCGGAGCACATCCGCTGCGACA
ACGGCCCTGAGATGATCTCGAAGGCACTGCGCAAGTGGGTCGCCAAAGCTGGCTCGCAAATCCAGTACATCGCGCCGGGCTCGCCGTGGGAGAACGGGTA
CTGTGAGAGCTTCAACGGCAAGCTGCGGGACGAGTGCCTGCGCCAAGAAATCTTTTACTCGCTCAAGGAAGCCCAGGCCGTGATCGCCCTTTGGCAGAAC
ACCTACAACCGTGTCTGGCCGCATTCCTCGCTGGGCTACCGGCCGCCCGCGCCTGTCAGCTTCCTGGATCTGGCCTTCTGGCTACCCATGGCCACCACCA
TGCAGTAGCCTCGCAACTGGCCCGGTCCAAAATACCGGTCAGGTCA
TCGTTTTGAAGCTGCGCCAGATCGAGGTGCAGACCGCCCAGGGCAAGAGTTTGGCGTTGGCGTGCAAGGAGGCGGAGATCTCCGAGCAGAGCTATTACCG
CTGGCGCAAGGAGTTCGGCGGCCTGCAGGTCGATCAAGCCAGGAAGATGAAGGACCTAGAGCGCGAGAACGCTCGCTTGCGGCGGCTTGTGGCTGACCTA
TCCCTGGAAAAGCAGGTGTTGGCGGACGTCGCCTCGGGAAACTTGTAGCCCCCGAGCGACGCCGGCAGGCGGTCGACGGTATCCGAGCCAAGTACGGCCT
CTCGGAACGTCACGCCTGCCGGATCGTCGGCCAGCATCGGGGTACGCAACGCTACGTGCCGACCGTGCTGGCCGACGAGGATGGGCTGACCCGAGCCATC
GTTGCCCTGGCGTCCGAGTACGGACGCTATGGCTACCGCCGCGTCACCGCGCTGCTGCAGGCAGCCGGCTGGCAGGTGGGCAAGGATCGGGTTCAGCGCA
TCTGGCGTCGCGAGGGGCTGAAGGTCCCCAGCCGACATAGACCCCGCAGCCGCCTCTGGCTGAACGACGGCTCATGCGTGCGACTACGGCCGCTTCGCCG
CAATCACGTCTGGAGCTTCGACTTCGTGCAGGCTCAGACGCACGATGGGCGAAGCCTGCGCATCCTGACGTTGATCGATGAGCACAGCCGGGCGTGCCTC
GCGCTGAAGGTGGCGCGGCGCATCAACAGCCTCGGTGTGATCGAGGCGCTGGCCGACGCCATGTGCCTGCATGGCATCCCGGAGCACATCCGCTGCGACA
ACGGCCCTGAGATGATCTCGAAGGCACTGCGCAAGTGGGTCGCCAAAGCTGGCTCGCAAATCCAGTACATCGCGCCGGGCTCGCCGTGGGAGAACGGGTA
CTGTGAGAGCTTCAACGGCAAGCTGCGGGACGAGTGCCTGCGCCAAGAAATCTTTTACTCGCTCAAGGAAGCCCAGGCCGTGATCGCCCTTTGGCAGAAC
ACCTACAACCGTGTCTGGCCGCATTCCTCGCTGGGCTACCGGCCGCCCGCGCCTGTCAGCTTCCTGGATCTGGCCTTCTGGCTACCCATGGCCACCACCA
TGCAGTAGCCTCGCAACTGGCCCGGTCCAAAATACCGGTCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 67 | 348 | + | No |
Description : First part of the transposase
ORF sequence :
MRRGQKTSAEQVVLKLRQIEVQTAQGKSLALACKEAEISEQSYYRWRKEFGGLQVDQARKMKDLERENARLRRLVADLSLEKQVLADVASGNL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 912 bp | 303 aa | 297 | 1208 | + | No |
Description : Second part of the transposase
ORF sequence :
PIPGKAGVGGRRLGKLVAPERRRQAVDGIRAKYGLSERHACRIVGQHRGTQRYVPTVLADEDGLTRAIVALASEYGRYGYRRVTALLQAAGWQVGKDRVQ
RIWRREGLKVPSRHRPRSRLWLNDGSCVRLRPLRRNHVWSFDFVQAQTHDGRSLRILTLIDEHSRACLALKVARRINSLGVIEALADAMCLHGIPEHIRC
DNGPEMISKALRKWVAKAGSQIQYIAPGSPWENGYCESFNGKLRDECLRQEIFYSLKEAQAVIALWQNTYNRVWPHSSLGYRPPAPVSFLDLAFWLPMAT
TMQ
RIWRREGLKVPSRHRPRSRLWLNDGSCVRLRPLRRNHVWSFDFVQAQTHDGRSLRILTLIDEHSRACLALKVARRINSLGVIEALADAMCLHGIPEHIRC
DNGPEMISKALRKWVAKAGSQIQYIAPGSPWENGYCESFNGKLRDECLRQEIFYSLKEAQAVIALWQNTYNRVWPHSSLGYRPPAPVSFLDLAFWLPMAT
TMQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1142 bp | 380 aa | 67 | 1208 | + | Yes |
Chemistry : DDE
ORF sequence :
MRRGQKTSAEQVVLKLRQIEVQTAQGKSLALACKEAEISEQSYYRWRKEFGGLQVDQARKMKDLERENARLRRLVADLSLEKQVLADVASGKLVAPERRR
QAVDGIRAKYGLSERHACRIVGQHRGTQRYVPTVLADEDGLTRAIVALASEYGRYGYRRVTALLQAAGWQVGKDRVQRIWRREGLKVPSRHRPRSRLWLN
DGSCVRLRPLRRNHVWSFDFVQAQTHDGRSLRILTLIDEHSRACLALKVARRINSLGVIEALADAMCLHGIPEHIRCDNGPEMISKALRKWVAKAGSQIQ
YIAPGSPWENGYCESFNGKLRDECLRQEIFYSLKEAQAVIALWQNTYNRVWPHSSLGYRPPAPVSFLDLAFWLPMATTMQ
QAVDGIRAKYGLSERHACRIVGQHRGTQRYVPTVLADEDGLTRAIVALASEYGRYGYRRVTALLQAAGWQVGKDRVQRIWRREGLKVPSRHRPRSRLWLN
DGSCVRLRPLRRNHVWSFDFVQAQTHDGRSLRILTLIDEHSRACLALKVARRINSLGVIEALADAMCLHGIPEHIRCDNGPEMISKALRKWVAKAGSQIQ
YIAPGSPWENGYCESFNGKLRDECLRQEIFYSLKEAQAVIALWQNTYNRVWPHSSLGYRPPAPVSFLDLAFWLPMATTMQ
Blast result :
Comments
ISMdi3 is 78% aa similar to ISCARN86.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References