ISMex32
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium extorquens | Methylobacterium extorquens AM1 |
DNA section
IS Length : 861 bp
Ends
IR Length : 16
IRL : GAGCGCCTAACAGAACGGGCACGGACCGCTTGGTCGGGCGTTGGTTGGTA
IRR : GAGCGCCTAACAGAACCGTTTGATCTGGTTGAGGGTGATGAGGCTTGCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCTCCCAAGCGGC | TAA | GCCATAAAACTTA | 3 |
DNA sequence
GAGCGCCTAACAGAACGGGCACGGACCGCTTGGTCGGGCGTTGGTTGGTATGGCACGTCCGCTTCTGCCCGATGATCTCTGGCGCGAGATTGCCCCGCAT
CTGCCGCCGCCCCGGCCACGTCCGAAGGGCGGCCGACGTCCGATCGAGAACCGGGCGGCGCTGACCGGTATCCTGTTCGTCCTGCGCTCCGGGTTGCCCT
GGGAGATGCTGCCGGCTGAAATGGGCTGCGGCTGCGGGATGAGCTGCTGGCGTCGGCTGCGCGACTGGCAGGAGGCGGGTGTCTGGGCGCGCCTGCATCA
GGTCCTGCTGGAGCGCCTGCACGCGGCCGGTGAGATCGACTGGAGCCGGGCGAGCCTGGACAGCGCATCTGTCCCGGCCAAAAAAGGGGCTCTGCCACCG
GCCCGAACCCGACGGACCGGGGCAAGCCGGGTACGAAGCGCCACCTCGTCACCGATGCGCGCGGCACGCCGCTCGGCTTCCGCCTGAGCGGGGCCAACCG
GCACGACAGCGTGATGATGGCCCAGACCCTCGACGCCATCCCGCCCTTGCGAAATGGACGGCGCGGGCGACCACGCAGGCGGCCCGGCAAGCTGCACGCA
GACAAGGCCTACGATGCCAGGGCGAGGCGCCAGGAGTGTCGGGCGCGCGGGATCGTACCGCGCATCGCCCGCAAAGGCATCGAGAGCAGCGAGCGGCTCG
GGCGTCACCGCTGGGTCGTCGAGCGGACCCACGCTTGGTTCAACAGCTTCCGTCGCCTGCCCATCCGCTACGAGCGGCGCAGCGACATCTACGAGGCCTT
CACCAGCCTCGCCGCAAGCCTCATCACCCTCAACCAGATCAAACGGTTCTGTTAGGCGCTC
CTGCCGCCGCCCCGGCCACGTCCGAAGGGCGGCCGACGTCCGATCGAGAACCGGGCGGCGCTGACCGGTATCCTGTTCGTCCTGCGCTCCGGGTTGCCCT
GGGAGATGCTGCCGGCTGAAATGGGCTGCGGCTGCGGGATGAGCTGCTGGCGTCGGCTGCGCGACTGGCAGGAGGCGGGTGTCTGGGCGCGCCTGCATCA
GGTCCTGCTGGAGCGCCTGCACGCGGCCGGTGAGATCGACTGGAGCCGGGCGAGCCTGGACAGCGCATCTGTCCCGGCCAAAAAAGGGGCTCTGCCACCG
GCCCGAACCCGACGGACCGGGGCAAGCCGGGTACGAAGCGCCACCTCGTCACCGATGCGCGCGGCACGCCGCTCGGCTTCCGCCTGAGCGGGGCCAACCG
GCACGACAGCGTGATGATGGCCCAGACCCTCGACGCCATCCCGCCCTTGCGAAATGGACGGCGCGGGCGACCACGCAGGCGGCCCGGCAAGCTGCACGCA
GACAAGGCCTACGATGCCAGGGCGAGGCGCCAGGAGTGTCGGGCGCGCGGGATCGTACCGCGCATCGCCCGCAAAGGCATCGAGAGCAGCGAGCGGCTCG
GGCGTCACCGCTGGGTCGTCGAGCGGACCCACGCTTGGTTCAACAGCTTCCGTCGCCTGCCCATCCGCTACGAGCGGCGCAGCGACATCTACGAGGCCTT
CACCAGCCTCGCCGCAAGCCTCATCACCCTCAACCAGATCAAACGGTTCTGTTAGGCGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 438 bp | 145 aa | 50 | 487 | + | No |
Description : First part of the transposase
ORF sequence :
MARPLLPDDLWREIAPHLPPPRPRPKGGRRPIENRAALTGILFVLRSGLPWEMLPAEMGCGCGMSCWRRLRDWQEAGVWARLHQVLLERLHAAGEIDWSR
ASLDSASVPAKKGALPPARTRRTGASRVRSATSSPMRAARRSASA
ASLDSASVPAKKGALPPARTRRTGASRVRSATSSPMRAARRSASA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 522 bp | 173 aa | 334 | 855 | + | No |
Description : Second part of the transposase
ORF sequence :
DRLEPGEPGQRICPGQKRGSATGPNPTDRGKPGTKRHLVTDARGTPLGFRLSGANRHDSVMMAQTLDAIPPLRNGRRGRPRRRPGKLHADKAYDARARRQ
ECRARGIVPRIARKGIESSERLGRHRWVVERTHAWFNSFRRLPIRYERRSDIYEAFTSLAASLITLNQIKRFC
ECRARGIVPRIARKGIESSERLGRHRWVVERTHAWFNSFRRLPIRYERRSDIYEAFTSLAASLITLNQIKRFC
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 806 bp | 268 aa | 50 | 855 | + | Yes |
Chemistry : DDE
ORF sequence :
MARPLLPDDLWREIAPHLPPPRPRPKGGRRPIENRAALTGILFVLRSGLPWEMLPAEMGCGCGMSCWRRLRDWQEAGVWARLHQVLLERLHAAGEIDWSR
ASLDSASVPAKKRGSATGPNPTDRGKPGTKRHLVTDARGTPLGFRLSGANRHDSVMMAQTLDAIPPLRNGRRGRPRRRPGKLHADKAYDARARRQECRAR
GIVPRIARKGIESSERLGRHRWVVERTHAWFNSFRRLPIRYERRSDIYEAFTSLAASLITLNQIKRFC
ASLDSASVPAKKRGSATGPNPTDRGKPGTKRHLVTDARGTPLGFRLSGANRHDSVMMAQTLDAIPPLRNGRRGRPRRRPGKLHADKAYDARARRQECRAR
GIVPRIARKGIESSERLGRHRWVVERTHAWFNSFRRLPIRYERRSDIYEAFTSLAASLITLNQIKRFC
Blast result :
Comments
ISMex32 is 73% aa similar to ISBcen20.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources.(2009) PLoS ONE Submitted.