ISMdi13

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium dichloromethanicum
Methylobacterium dichloromethanicum DM4
DNA section
IS Length : 1416 bp

Ends


IR Length : 24/31

IRL : GAGCTATCGCTGAAAGTTGGTGACGGCGGGAATCGGGAAGGCGGCATATC
IRR : GAGCTATCGCCAATTTTTGGTGACGGCCTGACCGGTCAGGCGGCGGCGGT

Insertion site


Left flankDirect repeatRight flankDR Length
CTTCATCGCATAGCGCCGCTGCCCAAAG8

DNA sequence

GAGCTATCGCTGAAAGTTGGTGACGGCGGGAATCGGGAAGGCGGCATATCGTGGGGGTGCTTGACGCCTCCACTTCTCCACCGAAGGAGCGATATGCCGT
GTCCAAAGATAACATCATCAAGCTGATTCAGCCAGGAAACGTCGACGATCAACTCACCGAAATCTTGCGCAATGGGGCGCGTGCTCTGTTGGCCCAGGCG
GTCGAGGCCGAGGTCGCGGACTTTCTCGGCAAGCATGCCGATTTGAAGACCGCGGACGGCCACCAGCGCGTCGTGCGCCACGGTCACCTGCCGGAACGCG
AGGTGATGACCGGTATTGGTCCGGTCGCCGTCCGCCAGCCGCGTGTACGCGATCGCGAGGCGGACGCCGCCGATCCCGACCGCATCCGGTTCTCTCCGTC
GACCTGCCGCCCTACATGCGCCGGTCGAAGTCGATCGAGACGCTGCTGCCGATCCTTTACCTGAAGGGTATCTCGACCGGCGACTTCTCCGAGGCGCTGG
CGGCGCTGCTCGGCAAGGATGCTGTCGGGTTGTCAGCATCCGCCATCGGCCGCCTGAAGGACGGCTGGCTCGACGAGCACACCGCGTGGCAGAAGCGCGA
TCTGTCGGTGAAGCGCTACGTCTACATCTGGGCCGATGGCATCCATCTCCAGGCGCGCCTCGAAGACGAAAAGCAGTGCATCCTGGTGCTGATCGGCGCG
ACGCCGGAAGGCCGCAAGGAACTGGTCGGCTTCACCGATGGTGCCCGCGAGAGCGCGCAGGACTGGCGCGATCTGCTGCTCGACCTGAAGCGGCGCGGGC
TCGACGTGCCGCCGCAGCTCGCCATCGCCGATGGCGCGCTCGGGTTCTGGAAGGCCGCCGGTGAGGTCTGGCCGAAAACGCGCGAGCAGCGCTGTTGGGT
GCACAAGACCGCGAACGTGCTCGCCAAGTTGCCGAAGAGCCAGCAGCCGAAGGCCAAACGCGCACTGCAGGAGATCTGGATGGCCGAAACCAAGGCCGCC
GCCGAGCTGGCGTTCGACGCCTTCATCGAGAGCTACACGCCCAAATACGAGAAGGCGGCCGACTGCCTGAGCAAGGACCGGGACACGCTACTGGCGTTCT
ACGACTTCCCGGCCGAGCACTGGAAACACCTGCGGACGACCAATCCCATTGAAAGCACCTTCGCTACCGTGCGCCACCGCACGATCCGATCGAAGGGGTG
CCTGTCCAACAGGACGGCGCTCGCGATGGTCTTCAAACTGCTCGAGGCCGCGCAGAAAAGCTGGCGTCGTCTCGATGGCCACAACCAGTTGCCAAAACTC
GTTCTCGGTGTGACATTCAACGACGGGATCGAGGTCATCGCCAAACCGAATGACCGCCAGCCCATAACCGCCGCCGCCTGACCGGTCAGGCCGTCACCAA
AAATTGGCGATAGCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1283 bp427 aa991381+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VSKDNIIKLIQPGNVDDQLTEILRNGARALLAQAVEAEVADFLGKHADLKTADGHQRVVRHGHLPEREVMTGIGPVAVRQPRVRDREADAADPDRIRFSP
STLPPYMRRSKSIETLLPILYLKGISTGDFSEALAALLGKDAVGLSASAIGRLKDGWLDEHTAWQKRDLSVKRYVYIWADGIHLQARLEDEKQCILVLIG
ATPEGRKELVGFTDGARESAQDWRDLLLDLKRRGLDVPPQLAIADGALGFWKAAGEVWPKTREQRCWVHKTANVLAKLPKSQQPKAKRALQEIWMAETKA
AAELAFDAFIESYTPKYEKAADCLSKDRDTLLAFYDFPAEHWKHLRTTNPIESTFATVRHRTIRSKGCLSNRTALAMVFKLLEAAQKSWRRLDGHNQLPK
LVLGVTFNDGIEVIAKPNDRQPITAAA

 

Blast result :
Comments
ISMdi13 is 76% aa similar to ISMex3. There is a ponctual mutation in the sequence. The transposase is reconstructed in silico : join(99-404;404-1381).
References
[1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources.(2009) PLoS ONE Submitted.