ISMex30

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
Methylobacterium dichloromethanicum DM4
DNA section
IS Length : 1047 bp

Ends


IR Length : 18/27

IRL : GAGCCGGTCCCACGCCGCTTGAGACGGGTTTGCGGGTAGCGCGTTGGTGG
IRR : GAGCTGTTCCCGGTCAGGTAGCGACGGCCAAGTCGTCTTCGTAGCCAGCT

Insertion site


Left flankDirect repeatRight flankDR Length
GCTCGGGGGCTAGTTGCGAGGC2
GCTCGGGGGCTAGTTGCGAGGC2

DNA sequence

GAGCCGGTCCCACGCCGCTTGAGACGGGTTTGCGGGTAGCGCGTTGGTGGGTGAAGGAGTTTGCTCATGCCTTCACCCCTGTCTGTGGACCTGCGCGAGC
GTGTCGTGGCTGCCGTGGCGGCGGGGGCTTCCTGCCATCGCACCGCAGCCCGCTTCGGGGTCAGCGTGTCGAGCGCCAGCCGCTGGTCGGAGCGCTTCCG
CCACGAGGGCCAACTCGCCTCCAAGCCGATGGGCGGCGATCACACCTCGAAGCGAATAGAGGCGCATGCCGGGCTGATCCTGAGGATCTCCAAGCAGGAG
CCGCGTCTTTTCCTGCGCGAGTTACGCGATCGGCTGGCTGAGCAGGGCATCCAGACCAGCACGAGCGGCCTGTCGCGCTTCTTTGCCCGCCACGGGATCA
GCTGGAAAAAGGGGCAACGTACGCAGCTGAGCAGGAGCGCGCGGACGTAAGAGCGGCTCGCGAGGCGTGGTTCGAGGCACAGCCCGAGTTCGATCCGGAT
CGGCTGGTGTTCCTGGATGAGACGGCGGCTGCCACCAACATGGCGCGCCGCTATGGTTGGGCTCCGTGCGGTGAGCGCTGCCGACTCGCAGCCCCGTTGG
GCCACTACAAAACCACCACCGTCACCGCCGCCCTGCGCACCAGCGGGCTGTGTGCCACCGCGCTATTGGATGGCCCCACGAACGGCAGGCGCTTCCGCAG
CTACCTCACCGAGACCCTGATCCCGGTGCTGCAGCCGGGCGACATCGTCGTTATGGATAACCTGCCGGCCCACAAGGTCGCCGGCGTGCAGGACGCGATC
GAGGCCGCAGGAGCGCGATTGCTCTACCTTCCGTCCTACAGCCCTGATTTCAACCCGATTGAGCAGGCCTTCGCGAAGCTGAAGGCGCTGCTACGCAGCG
CGGCAGCCCGCACGATCCCGGATCTCTGGGCTGCGATCCGCCAGGCATTCACGCGCTTCACTCCGCAGGAGTGCCGCAACTACCTCGCCGCAGCTGGCTA
CGAAGACGACTTGGCCGTCGCTACCTGACCGGGAACAGCTC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
384 bp127 aa67450+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPSPLSVDLRERVVAAVAAGASCHRTAARFGVSVSSASRWSERFRHEGQLASKPMGGDHTSKRIEAHAGLILRISKQEPRLFLRELRDRLAEQGIQTSTS
GLSRFFARHGISWKKGQRTQLSRSART

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
687 bp228 aa3421028+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

AGHPDQHERPVALLCPPRDQLEKGATYAAEQERADVRAAREAWFEAQPEFDPDRLVFLDETAAATNMARRYGWAPCGERCRLAAPLGHYKTTTVTAALRT
SGLCATALLDGPTNGRRFRSYLTETLIPVLQPGDIVVMDNLPAHKVAGVQDAIEAAGARLLYLPSYSPDFNPIEQAFAKLKALLRSAAARTIPDLWAAIR
QAFTRFTPQECRNYLAAAGYEDDLAVAT

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
962 bp320 aa671028+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPSPLSVDLRERVVAAVAAGASCHRTAARFGVSVSSASRWSERFRHEGQLASKPMGGDHTSKRIEAHAGLILRISKQEPRLFLRELRDRLAEQGIQTSTS
GLSRFFARHGISWKKGATYAAEQERADVRAAREAWFEAQPEFDPDRLVFLDETAAATNMARRYGWAPCGERCRLAAPLGHYKTTTVTAALRTSGLCATAL
LDGPTNGRRFRSYLTETLIPVLQPGDIVVMDNLPAHKVAGVQDAIEAAGARLLYLPSYSPDFNPIEQAFAKLKALLRSAAARTIPDLWAAIRQAFTRFTP
QECRNYLAAAGYEDDLAVAT

 

Blast result :
Comments
ISMex30 is 84% aa similar to ISMex21.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References