ISMex27

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
DNA section
IS Length : 2493 bp

Ends


IR Length : 30

IRL : TGTTGATTTCCGCTGAGAGTTGACCCGGCGGAGCCCGAAGTTTCCATCGA
IRR : TGTTGATTTCCGCTGAGAGTTGACCCGGCGTTTTCGTTGAGAAGTGACCC

Insertion site


Left flankDirect repeatRight flankDR Length
GGCGCCAGCTCCGTGGCGTT0

DNA sequence

TGTTGATTTCCGCTGAGAGTTGACCCGGCGGAGCCCGAAGTTTCCATCGAGAAGTGACCCGTGTCTGAACTCACCCCTGGCTTGCTGGTCGGGGGTCATG
GAGTGATCGACATGGCATTATTGAGCGTCATCCGGCGCTGGGCATTTCGAGAAGGGATGCCGATCCGGGAGATTTCGCGACGGACAGGATTGTCGCGCAA
CACCATTCGCAAATATCTGCGATCGGACGCGCAGGAGCCGGTGTATCGAGCGCCGGACCGGCCCAGCAGGTTGGACCCGTTCGCCGCAAAACTGTCAGGC
TGGTTGAAGGCCGAGGCGAACCGGTCCCGCAAGCAACAGCGTACGGCGCGGCAGATGCACGCCGACTTGGTGCTCCTCGGCTATGACGGCTCCTACGGCA
GGGTCGCGGCCTTTATTCGACGCTGGAAGGAGAACCGGCAGACCGAACAGCAGACCACGGGACGCGGGACCTTCGTACCTCTGATCTTCGCGCCCGGTGA
AGCGTTCCAGTTCGACTGGAGCGAGGACTGGGCGGATCTCGCAGGCGAGCGCGTCAAGCTGCAGGTGGCGCACACCAAGCTCTCGCACAGTCGAGCCTTT
GTCGTGCGCGCCTACCCGCTTCAGAGCCACGAGATGCTGTTCGACGCGCACTACCATGCCTTCCGCGTGCTCGGCGGTATCCCGGGTCGCGGCATCTACG
ACAACATGAAGACCGCCGTGGATCGGGTCGGCTCCGGCAAGGCCCGGCAGGTGAACGCGCGCTTCGCCGCCATGGCGAGCCACTATCTATTCGACACGAC
CTTCTGCAATCCCGCCTCAGGCTGGGAGAAGGGGCAGGTCGAGAAGAACGTGCAGGACGCGCGTCGCCGGCTGTGGCAGCCGCTCCCCAGCTTCGCCGAT
CTCGATGGGTTGAACGCCTGGCTTGAGATGCGCTGCATCGCGGCCTGGAAGGAGATCCCGCATGGAGATCTCCCCGGCACGCTCGCCGATGCGTGGAGCG
CCGAGGTCGCGAGCCTGATGCCGCTCGGGCGACCGTTCGACGGCTTCGTCGAGCACACAAAGCGGGTCTCGCCGACCTGCCTCGTGCACTTCGAGCGCAA
CCGCTATTCGGTTCCGGCATCGCTTGCCAATCGCCCCGTAAGCGTGAGGATCTACCCCGAGCGCATCGTCGTGGCCGCAGAAGGCCGGATCCTGTGCGAG
CATCTGCGAGTCATATCGCGCTCGCACAGCGTGGCCGGGCGCACGATCTACGACTGGCGCCACTATCTGGCGGTCATCCAACGCAAGCCAGGCGCCCTGC
GCAATGGGGCTCCCTTCGCCGAGATGCCGGATGCATTCCGGCGTTTGCAGGGGCACCTCCTCAAGCACCCTGGCGGCGATCGGGAGATGGTAGAGATTCT
GGCACTCGTCCTGCAGCACGACGAGCAGGCTGTTCTATGCGCGGTCGAACTGGCGCTCGAGAGCGGCGTCCCCACCAAGATCCATGTTCTCAACATCCTG
CACCGGCTGCTCGACGGTAAGCCATCGAGCATGCCAACCATCGATGCCCCGCAGGCGCTCGTCTTGCGTCAGGAGCCGAAGGCCGATGTCGAGCGCTACG
ACGCGCTCCGTAGCAAGGCGGTCCGCCATGCGTCATGACCCCGCCAGCGCCGCCATCATCGTCATGCTGCGCGGGCTCAAGATGTACGGCATGTCTCAAG
CCGTCGGCGACCTTGTGGAGCAAGGCGCGCCTGCCTTCGAGGCAGCCGTCCCGCTCTTGGCCCAGTTGCTCAAGGCCGAGATGGCCGAGCGGGAGGTCCG
ATCCATCGCCTACCAGATCAAGGCCGCCCGCTTTCCGGCCTACAAGGACCTGACCGGGTTCGACTTCGCTGCCAGCGAGGTCAACGAGGCCGTGGTGCGC
ACGCTGCACGGGGGCGACTTCATCGACGGGGCACACAACGTTGTGCTGATCGGGGGGCCAGGAACCGGCAAAACGCACGTCGCAACGGCCCTAGGCGTGC
AGGCCATCGAGCACCATCGCAAGAAGGTCCGATTCTTCGCCACCGTCGACCTGGTCAATGCGCTCGAACAGGAGAAGGCCCTGAACAAGGCCGGCCAATT
GGCGGATCGATTGCTGCGCCTGGACCTGATCATCCTGGACGAGCTGGGCTATCTCCCCTTCAGCACCTCAGGAGGAGCCTTGCTGTTCCACCTGCTGTCC
AAGCTCTACGAGCGCACCAGCGTCGTCATCACGACCAACCTCAGCTTCAGCGAGTGGGCTGACATCTTCGGCGATGCCAAGATGACCACTGCGTTGCTCG
ATCGCCTCACCCACCACTGCCACATTCTGGAAACCGGAAACGACAGCTTCCGCTTCCGGGCCAGTGCCGCAGCCGGAAAACCAAAAAGGAGCCGCGCTGA
GTTGACCAAGCCCGTCCAATGACAAACATCAGCCCCTGAACCCGGGTCACTTCTCAACGAAAACGCCGGGTCAACTCTCAGCGGAAATCAACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1449 bp482 aa1901638+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

LSRNTIRKYLRSDAQEPVYRAPDRPSRLDPFAAKLSGWLKAEANRSRKQQRTARQMHADLVLLGYDGSYGRVAAFIRRWKENRQTEQQTTGRGTFVPLIF
APGEAFQFDWSEDWADLAGERVKLQVAHTKLSHSRAFVVRAYPLQSHEMLFDAHYHAFRVLGGIPGRGIYDNMKTAVDRVGSGKARQVNARFAAMASHYL
FDTTFCNPASGWEKGQVEKNVQDARRRLWQPLPSFADLDGLNAWLEMRCIAAWKEIPHGDLPGTLADAWSAEVASLMPLGRPFDGFVEHTKRVSPTCLVH
FERNRYSVPASLANRPVSVRIYPERIVVAAEGRILCEHLRVISRSHSVAGRTIYDWRHYLAVIQRKPGALRNGAPFAEMPDAFRRLQGHLLKHPGGDREM
VEILALVLQHDEQAVLCAVELALESGVPTKIHVLNILHRLLDGKPSSMPTIDAPQALVLRQEPKADVERYDALRSKAVRHAS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
795 bp264 aa16282422+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRHDPASAAIIVMLRGLKMYGMSQAVGDLVEQGAPAFEAAVPLLAQLLKAEMAEREVRSIAYQIKAARFPAYKDLTGFDFAASEVNEAVVRTLHGGDFID
GAHNVVLIGGPGTGKTHVATALGVQAIEHHRKKVRFFATVDLVNALEQEKALNKAGQLADRLLRLDLIILDELGYLPFSTSGGALLFHLLSKLYERTSVV
ITTNLSFSEWADIFGDAKMTTALLDRLTHHCHILETGNDSFRFRASAAAGKPKRSRAELTKPVQ

 

Blast result :
Comments
ISMex27 is 85% (istA, the transposase) and 94% (istB, the helper of transposition) aa similar to ISRsp3.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.