ISMex41
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium extorquens | Methylobacterium extorquens PA1 |
DNA section
IS Length : 1054 bp
Ends
IR Length : 19/26
IRL : GAGCGTTTTCGGTTTGGGCTGAGTCGGCTTGGTGAGGTTCACAGGGCTTT
IRR : GAGCATTTTCGGTTTAATCGGGATCGTACCCAGCGGCTGTGAAGAAGTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCGATCTGC | TA | GGTTGGGGTA | 2 |
DNA sequence
GAGCGTTTTCGGTTTGGGCTGAGTCGGCTTGGTGAGGTTCACAGGGCTTTTGCCGGCTGATTCAGTGCTCGCTCCGGACGAGGAGCGAGACATGGGCCGA
CCCTACAGCCTGGACCTGCGCCAGCGGGTGGTCGATGCGGCGGCCTCGACCTCACGGCGTCAGGCCGCCGCCCGCTTCGGGGTCGGGATCGCCACGGCGA
TCCGCTGGGCCGCCGCACTCGTGGCCACCGGCACGGTGGCAGCCCGATCCCAGGGCCGTCCCCGCACCTCGAAGCTTGATCCGCACGAGGCGTTCCTGCG
CGGCCTGATCGACGGACAAGACGATATCACCCTGGAGGAGATGCGCGCCCGGCTGTCGGCCGAGCGTGATCTGAAGGTCGGATTGGGCACGCTGTGGGCC
TTTCTCGACGCGCGCGACCTCACGTACAAAAAAAGACAGCCCATGCCGCCGAGCAGGAGCGGCCGGACGTGAAGGCAGCGCGGGAAGCTTGGTTCGAGGG
CCAGCCCGATTTCGACCCGACCCGTTTGGTCTTCATCGACGAGACTTGGACAGCGACCAACATGGTCCGCACCCACGGGCGTTGTCGGCGCGGTCAGCGC
TTGCGCTCACCAGTCCCGCACGGCCACTGGAAGACAACGACCTTCGTGGCGGGCTTGCGTCTGTCCGGGATTGCCGCACCGTTCGTGCTCGACGGGCCGA
TCAACCGCGTGGCCTTCCAGACCTTTGTCGAACAGGTGCTGGTGCCCGAACTCACGCCCGGCGACATCGTGGTGATGGACAACCTCGGCAGCCACAAGGG
CGCGGCCGTTCGGACCGCTATTGAGGCAGCAGGCGCACGGCTCTTGTTCCTGCCGCCCTACAGCCCGGACTTCAACCCGATCGAGATGGCCTTCTCCAAG
CTCAAGGCGCTGCTGCGCAAGGCGGCCGAGCGCACCGTCGAAGGGCTCTGGTCGGCCATCGGACGCCTCATCGACACCGTCACCACCAATGAGTGCGAAA
ACTTCTTCACAGCCGCTGGGTACGATCCCGATTAAACCGAAAATGCTC
CCCTACAGCCTGGACCTGCGCCAGCGGGTGGTCGATGCGGCGGCCTCGACCTCACGGCGTCAGGCCGCCGCCCGCTTCGGGGTCGGGATCGCCACGGCGA
TCCGCTGGGCCGCCGCACTCGTGGCCACCGGCACGGTGGCAGCCCGATCCCAGGGCCGTCCCCGCACCTCGAAGCTTGATCCGCACGAGGCGTTCCTGCG
CGGCCTGATCGACGGACAAGACGATATCACCCTGGAGGAGATGCGCGCCCGGCTGTCGGCCGAGCGTGATCTGAAGGTCGGATTGGGCACGCTGTGGGCC
TTTCTCGACGCGCGCGACCTCACGTACAAAAAAAGACAGCCCATGCCGCCGAGCAGGAGCGGCCGGACGTGAAGGCAGCGCGGGAAGCTTGGTTCGAGGG
CCAGCCCGATTTCGACCCGACCCGTTTGGTCTTCATCGACGAGACTTGGACAGCGACCAACATGGTCCGCACCCACGGGCGTTGTCGGCGCGGTCAGCGC
TTGCGCTCACCAGTCCCGCACGGCCACTGGAAGACAACGACCTTCGTGGCGGGCTTGCGTCTGTCCGGGATTGCCGCACCGTTCGTGCTCGACGGGCCGA
TCAACCGCGTGGCCTTCCAGACCTTTGTCGAACAGGTGCTGGTGCCCGAACTCACGCCCGGCGACATCGTGGTGATGGACAACCTCGGCAGCCACAAGGG
CGCGGCCGTTCGGACCGCTATTGAGGCAGCAGGCGCACGGCTCTTGTTCCTGCCGCCCTACAGCCCGGACTTCAACCCGATCGAGATGGCCTTCTCCAAG
CTCAAGGCGCTGCTGCGCAAGGCGGCCGAGCGCACCGTCGAAGGGCTCTGGTCGGCCATCGGACGCCTCATCGACACCGTCACCACCAATGAGTGCGAAA
ACTTCTTCACAGCCGCTGGGTACGATCCCGATTAAACCGAAAATGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 381 bp | 126 aa | 92 | 472 | + | No |
Description : First part of the transposase
ORF sequence :
MGRPYSLDLRQRVVDAAASTSRRQAAARFGVGIATAIRWAAALVATGTVAARSQGRPRTSKLDPHEAFLRGLIDGQDDITLEEMRARLSAERDLKVGLGT
LWAFLDARDLTYKKRQPMPPSRSGRT
LWAFLDARDLTYKKRQPMPPSRSGRT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 666 bp | 221 aa | 370 | 1035 | + | No |
Description : Second part of the transposase
ORF sequence :
SEGRIGHAVGLSRRARPHVQKKTAHAAEQERPDVKAAREAWFEGQPDFDPTRLVFIDETWTATNMVRTHGRCRRGQRLRSPVPHGHWKTTTFVAGLRLSG
IAAPFVLDGPINRVAFQTFVEQVLVPELTPGDIVVMDNLGSHKGAAVRTAIEAAGARLLFLPPYSPDFNPIEMAFSKLKALLRKAAERTVEGLWSAIGRL
IDTVTTNECENFFTAAGYDPD
IAAPFVLDGPINRVAFQTFVEQVLVPELTPGDIVVMDNLGSHKGAAVRTAIEAAGARLLFLPPYSPDFNPIEMAFSKLKALLRKAAERTVEGLWSAIGRL
IDTVTTNECENFFTAAGYDPD
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 944 bp | 314 aa | 92 | 1035 | + | Yes |
Chemistry : DDE
ORF sequence :
MGRPYSLDLRQRVVDAAASTSRRQAAARFGVGIATAIRWAAALVATGTVAARSQGRPRTSKLDPHEAFLRGLIDGQDDITLEEMRARLSAERDLKVGLGT
LWAFLDARDLTYKKKTAHAAEQERPDVKAAREAWFEGQPDFDPTRLVFIDETWTATNMVRTHGRCRRGQRLRSPVPHGHWKTTTFVAGLRLSGIAAPFVL
DGPINRVAFQTFVEQVLVPELTPGDIVVMDNLGSHKGAAVRTAIEAAGARLLFLPPYSPDFNPIEMAFSKLKALLRKAAERTVEGLWSAIGRLIDTVTTN
ECENFFTAAGYDPD
LWAFLDARDLTYKKKTAHAAEQERPDVKAAREAWFEGQPDFDPTRLVFIDETWTATNMVRTHGRCRRGQRLRSPVPHGHWKTTTFVAGLRLSGIAAPFVL
DGPINRVAFQTFVEQVLVPELTPGDIVVMDNLGSHKGAAVRTAIEAAGARLLFLPPYSPDFNPIEMAFSKLKALLRKAAERTVEGLWSAIGRLIDTVTTN
ECENFFTAAGYDPD
Blast result :
Comments
ISMex41 is 69% aa similar to ISRm10-1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References