ISMex21
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium extorquens | Methylobacterium extorquens AM1 Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1040 bp
Ends
IR Length : 21/27
IRL : GAGCTGTTCCCGGCGATGTTGCGACGGGACGGTGGTTGGGGCGTTGACCG
IRR : GAGCCGTTCCCGCTTACGTAGCGACGGCCAAATCGTTGTCGTAGCCGGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCAAGCAGC | TA | GAGTCATTGA | 2 |
| CTCGGCTAAC | TA | GCTCACTCGC | 2 |
| TGCCTGCCAC | TA | GTGACTAATT | 2 |
| AGCAGGATGC | TA | GTACGGCGCG | 2 |
DNA sequence
GAGCTGTTCCCGGCGATGTTGCGACGGGACGGTGGTTGGGGCGTTGACCGGTGAAGGAGCCAGCGATGCCTTCACCGTTGTCTGTCGATCTGCGCGAACG
CGTCGTAGCGGCCGTGGCCGGGGGCGCTTCCTTCCATTGGGCGGCGGCGCGGTTCGGGGTGAGCGTGTCGAGCGCCAGCCGCTGGTCGCAGCGGTTTGCG
CAGCAGGGTCACGTCGCGCCCAAACCGACTCACGCCGACGACAGCCTGCCGGCGATCGAGGCGCATGCGGATCTGATCCTCTCCCTCGATGAGGCGCAGC
CCGAACTCTTCCTGCGCGAATTGCGCGACGCCCTGGCCGAGCAGGGCGTGCAGACCAGCACGAGCGGGCTGTCACGCTTCTTCGCTCGCCACGGCATCAC
CCGCAAAAAGGGGCGCAGTACGCGGCTGAGCAGGAGCGGGCCGACGTGAAGGCGGCGCGTGAGGCGTGGTTCGAGGCTCAGCCCGACCTCGACCCCGACA
AGCTCGTCTTCCTCGACGAGACGGCGGCTGCCACCAACATGGCCCGCCGCTACGGCCGCGCCCCGCGCGGCGAGCGCTGCCGGCTCCTCGTACCGCAGGG
CCATTCTAAAACGACTACTGTCACCGCCGCTCTGCGCACCACCGGCCTGTGCGCCCTCGACCTCGCGGATGGCGCCACAAACGGCCAGCGCTTTCGCGAC
TACGTCGCCAACCGCCTCGTCCCGGTGCTGCGGCCCGGCGACACCGTGATCCTCGACAACCTGCAAGCTCACAAGGTCAGCGGCATCCGCGAGCGGGTCG
AAGCGGCCGGCGCGCGGCTGCTCTACCTTCCGCCCTACAGCCCCGAGTTCAACCCGATCGAACAGATCTTCGCCAAGCTGAAAGCGCTGTTGCGGACCGC
AGCCGCCCGCACCGTGGCTGACCTCTGGGACGCCATCCGGAACGCCTTCACCCGCTTCACCCCAGACGAATGCTGCAACTGCCTCGCCGCCGCCGGCTAC
GACAACGATTTGGCCGTCGCTACGTAAGCGGGAACGGCTC
CGTCGTAGCGGCCGTGGCCGGGGGCGCTTCCTTCCATTGGGCGGCGGCGCGGTTCGGGGTGAGCGTGTCGAGCGCCAGCCGCTGGTCGCAGCGGTTTGCG
CAGCAGGGTCACGTCGCGCCCAAACCGACTCACGCCGACGACAGCCTGCCGGCGATCGAGGCGCATGCGGATCTGATCCTCTCCCTCGATGAGGCGCAGC
CCGAACTCTTCCTGCGCGAATTGCGCGACGCCCTGGCCGAGCAGGGCGTGCAGACCAGCACGAGCGGGCTGTCACGCTTCTTCGCTCGCCACGGCATCAC
CCGCAAAAAGGGGCGCAGTACGCGGCTGAGCAGGAGCGGGCCGACGTGAAGGCGGCGCGTGAGGCGTGGTTCGAGGCTCAGCCCGACCTCGACCCCGACA
AGCTCGTCTTCCTCGACGAGACGGCGGCTGCCACCAACATGGCCCGCCGCTACGGCCGCGCCCCGCGCGGCGAGCGCTGCCGGCTCCTCGTACCGCAGGG
CCATTCTAAAACGACTACTGTCACCGCCGCTCTGCGCACCACCGGCCTGTGCGCCCTCGACCTCGCGGATGGCGCCACAAACGGCCAGCGCTTTCGCGAC
TACGTCGCCAACCGCCTCGTCCCGGTGCTGCGGCCCGGCGACACCGTGATCCTCGACAACCTGCAAGCTCACAAGGTCAGCGGCATCCGCGAGCGGGTCG
AAGCGGCCGGCGCGCGGCTGCTCTACCTTCCGCCCTACAGCCCCGAGTTCAACCCGATCGAACAGATCTTCGCCAAGCTGAAAGCGCTGTTGCGGACCGC
AGCCGCCCGCACCGTGGCTGACCTCTGGGACGCCATCCGGAACGCCTTCACCCGCTTCACCCCAGACGAATGCTGCAACTGCCTCGCCGCCGCCGGCTAC
GACAACGATTTGGCCGTCGCTACGTAAGCGGGAACGGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 384 bp | 127 aa | 66 | 449 | + | No |
Description : First part of the transposase
ORF sequence :
MPSPLSVDLRERVVAAVAGGASFHWAAARFGVSVSSASRWSQRFAQQGHVAPKPTHADDSLPAIEAHADLILSLDEAQPELFLRELRDALAEQGVQTSTS
GLSRFFARHGITRKKGRSTRLSRSGPT
GLSRFFARHGITRKKGRSTRLSRSGPT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 735 bp | 244 aa | 293 | 1027 | + | No |
Description : Second part of the transposase
ORF sequence :
GAARTLPARIARRPGRAGRADQHERAVTLLRSPRHHPQKGAQYAAEQERADVKAAREAWFEAQPDLDPDKLVFLDETAAATNMARRYGRAPRGERCRLLV
PQGHSKTTTVTAALRTTGLCALDLADGATNGQRFRDYVANRLVPVLRPGDTVILDNLQAHKVSGIRERVEAAGARLLYLPPYSPEFNPIEQIFAKLKALL
RTAAARTVADLWDAIRNAFTRFTPDECCNCLAAAGYDNDLAVAT
PQGHSKTTTVTAALRTTGLCALDLADGATNGQRFRDYVANRLVPVLRPGDTVILDNLQAHKVSGIRERVEAAGARLLYLPPYSPEFNPIEQIFAKLKALL
RTAAARTVADLWDAIRNAFTRFTPDECCNCLAAAGYDNDLAVAT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 962 bp | 320 aa | 66 | 1027 | + | Yes |
Chemistry : DDE
ORF sequence :
MPSPLSVDLRERVVAAVAGGASFHWAAARFGVSVSSASRWSQRFAQQGHVAPKPTHADDSLPAIEAHADLILSLDEAQPELFLRELRDALAEQGVQTSTS
GLSRFFARHGITRKKGAQYAAEQERADVKAAREAWFEAQPDLDPDKLVFLDETAAATNMARRYGRAPRGERCRLLVPQGHSKTTTVTAALRTTGLCALDL
ADGATNGQRFRDYVANRLVPVLRPGDTVILDNLQAHKVSGIRERVEAAGARLLYLPPYSPEFNPIEQIFAKLKALLRTAAARTVADLWDAIRNAFTRFTP
DECCNCLAAAGYDNDLAVAT
GLSRFFARHGITRKKGAQYAAEQERADVKAAREAWFEAQPDLDPDKLVFLDETAAATNMARRYGRAPRGERCRLLVPQGHSKTTTVTAALRTTGLCALDL
ADGATNGQRFRDYVANRLVPVLRPGDTVILDNLQAHKVSGIRERVEAAGARLLYLPPYSPEFNPIEQIFAKLKALLRTAAARTVADLWDAIRNAFTRFTP
DECCNCLAAAGYDNDLAVAT
Blast result :
Comments
ISMex21 is 78% aa similar to ISMdi20.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References