ISMex18
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium extorquens | Methylobacterium extorquens AM1 Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1167 bp
Ends
IR Length : 22/25
IRL : GTGGCCTGAGTTGGAAGTTTCCTTGCGGTTTTGGCGCGCGAGCACCATGT
IRR : GTGGTCTGAGTTGGAAGTTCGTTGGCAGAAGCGTTTGATGCTGGCGAGGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGAGACGTC | TA | GGACGTTCAT | 2 |
| CTCGGCTTCC | TA | GCTTGTAACC | 2 |
DNA sequence
GTGGCCTGAGTTGGAAGTTTCCTTGCGGTTTTGGCGCGCGAGCACCATGTGGCGGCCTTCTACGAGGAGGTCGCTACGATGGCCCGTGGTCCCAAGCCAG
CGCACCTTGATCTGACCGGGGATGAGCGCACGCGGCTGCAAGGCCTGGTGCGCCGTCGCAATGTCGGCCAGGCTCTCGCCCAGCGTGCGCGCATCGTGCT
GGCCTGCGCTGAGCCGGGTTCGACCAACAGCGGCATCGCTCGTGCGCTCGGCGTCAGCCGCATGAGCGTCACGACATGGCGCGCCCGTTTCCTGGCGCAC
CGCCTCGACGGTCTCGTCGACCTGCCCCGGTCCGGCGCTCCGCGCCAGGTCGCGGATACCGAGATCGAACAGATGATCACGCGCACCCTGGAGAGCCAGC
CGGAGAACGCCACCCACTGGTCGACACGCGCCATGGCCCGGCGGGTCGGCATGAGCCAGACCATGGTCTCGCGGATCTGGCGTGCCTTCGGGCTGCAGCC
GCACAAGATCGAGGCCTTCAAACTGTCGACCGACCCGGCTTTCGTCGACAAGGTCCGGGATGTCGTCGGCCTCTACATGGCCCCACCGCACCGCGCCGTG
GTGCTGTGTGTCGATGAGAAGCCGCAGATCCAGGCGCTGACCGGCACCGCGCCGGTGATGCCGATGCGCCCCGGCCAGCCCGAGCGCCAGACCCACGACT
ACCGACGGGCTGGTACGACCGACCTGTTCGCGGCGCTCGACGTACAGGCCGGTCGCGTCATCGGCCATTGCGCACGGCGTCATCGCTCCATCGAGTTCCG
CGCCTTTCTCGATCAGGTCGAGGCCGATGTGCCGAAGGATCTCGATGTGCACCTGGTTCTCGACAACGCGGCCACACACAAGACCAAGCTCATCCACGAC
TGGCTGCTGAAGCGTCCGCGCTGGCACCTGCACTTCACGCCGACCTCGGCCTCTTGGCTCAACCTCGTCGAGGGCTGGTTCGCGCTGCTGACGCGGCGCC
AGCTTCAACGCGGCGTGTTCGAGACCACGGGCGATCTTGAAGCCGCCATCCACGCCTACATCGACCAGACCAACGCCGAGCCGAAGCCGTTCGTCTGGAC
CAAGTCAGCCGACGCCATCCTCGCCAGCATCAAACGCTTCTGCCAACGAACTTCCAACTCAGACCAC
CGCACCTTGATCTGACCGGGGATGAGCGCACGCGGCTGCAAGGCCTGGTGCGCCGTCGCAATGTCGGCCAGGCTCTCGCCCAGCGTGCGCGCATCGTGCT
GGCCTGCGCTGAGCCGGGTTCGACCAACAGCGGCATCGCTCGTGCGCTCGGCGTCAGCCGCATGAGCGTCACGACATGGCGCGCCCGTTTCCTGGCGCAC
CGCCTCGACGGTCTCGTCGACCTGCCCCGGTCCGGCGCTCCGCGCCAGGTCGCGGATACCGAGATCGAACAGATGATCACGCGCACCCTGGAGAGCCAGC
CGGAGAACGCCACCCACTGGTCGACACGCGCCATGGCCCGGCGGGTCGGCATGAGCCAGACCATGGTCTCGCGGATCTGGCGTGCCTTCGGGCTGCAGCC
GCACAAGATCGAGGCCTTCAAACTGTCGACCGACCCGGCTTTCGTCGACAAGGTCCGGGATGTCGTCGGCCTCTACATGGCCCCACCGCACCGCGCCGTG
GTGCTGTGTGTCGATGAGAAGCCGCAGATCCAGGCGCTGACCGGCACCGCGCCGGTGATGCCGATGCGCCCCGGCCAGCCCGAGCGCCAGACCCACGACT
ACCGACGGGCTGGTACGACCGACCTGTTCGCGGCGCTCGACGTACAGGCCGGTCGCGTCATCGGCCATTGCGCACGGCGTCATCGCTCCATCGAGTTCCG
CGCCTTTCTCGATCAGGTCGAGGCCGATGTGCCGAAGGATCTCGATGTGCACCTGGTTCTCGACAACGCGGCCACACACAAGACCAAGCTCATCCACGAC
TGGCTGCTGAAGCGTCCGCGCTGGCACCTGCACTTCACGCCGACCTCGGCCTCTTGGCTCAACCTCGTCGAGGGCTGGTTCGCGCTGCTGACGCGGCGCC
AGCTTCAACGCGGCGTGTTCGAGACCACGGGCGATCTTGAAGCCGCCATCCACGCCTACATCGACCAGACCAACGCCGAGCCGAAGCCGTTCGTCTGGAC
CAAGTCAGCCGACGCCATCCTCGCCAGCATCAAACGCTTCTGCCAACGAACTTCCAACTCAGACCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1119 bp | 373 aa | 49 | 1167 | + | No |
Chemistry : DDE
ORF sequence :
VAAFYEEVATMARGPKPAHLDLTGDERTRLQGLVRRRNVGQALAQRARIVLACAEPGSTNSGIARALGVSRMSVTTWRARFLAHRLDGLVDLPRSGAPRQ
VADTEIEQMITRTLESQPENATHWSTRAMARRVGMSQTMVSRIWRAFGLQPHKIEAFKLSTDPAFVDKVRDVVGLYMAPPHRAVVLCVDEKPQIQALTGT
APVMPMRPGQPERQTHDYRRAGTTDLFAALDVQAGRVIGHCARRHRSIEFRAFLDQVEADVPKDLDVHLVLDNAATHKTKLIHDWLLKRPRWHLHFTPTS
ASWLNLVEGWFALLTRRQLQRGVFETTGDLEAAIHAYIDQTNAEPKPFVWTKSADAILASIKRFCQRTSNSDH
VADTEIEQMITRTLESQPENATHWSTRAMARRVGMSQTMVSRIWRAFGLQPHKIEAFKLSTDPAFVDKVRDVVGLYMAPPHRAVVLCVDEKPQIQALTGT
APVMPMRPGQPERQTHDYRRAGTTDLFAALDVQAGRVIGHCARRHRSIEFRAFLDQVEADVPKDLDVHLVLDNAATHKTKLIHDWLLKRPRWHLHFTPTS
ASWLNLVEGWFALLTRRQLQRGVFETTGDLEAAIHAYIDQTNAEPKPFVWTKSADAILASIKRFCQRTSNSDH
Blast result :
Comments
ISMex18 is 71% aa similar to ISMsm2.
There is no stop codon at the end of the element, the transposase use the first STOP codon after the end of the IRR. The transposase in the database is cut at the end of the IS.
There is no stop codon at the end of the element, the transposase use the first STOP codon after the end of the IRR. The transposase in the database is cut at the end of the IS.
References