ISMex14
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium extorquens | Methylobacterium extorquens AM1 |
DNA section
IS Length : 1381 bp
Ends
IR Length : 25/33
IRL : GGTAGCGTCCGCGGACGAGGTGTAAATTCGAGGACCGCTTTGGCGGGGAT
IRR : GGTCGTGTCCGTCAATGAGGTGGAAATTCGGGGTGGAGTTGGAGCGGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGCGGTGGG | GTTTTTCA | CGTGCCTACC | 8 |
| GATCGGGCCA | CCAGGCGGC | AGCATCGGCC | 9 |
DNA sequence
GGTAGCGTCCGCGGACGAGGTGTAAATTCGAGGACCGCTTTGGCGGGGATTGGGGTGGCCATCGGGCCATGCGGCTAAGGTGGAGTTTGCGGACTTCAAC
CCGAACCGGGAGCACCCGATGACCGACGACAGAGTGGCACTGATCGAGGCTTTTCAGAAGGCTGACGACGGCAACTTCCTGCGCAGCCTGGCCGAGACGG
TCCTGCAGATCCTGATGGAGGCCGATGTGGACGGTCTGATCGGCGCGGGTCGTTACGAGCGGTCTGGCGAGCGCAGCACCTACCGCAACGGGTATCGCGA
ACGCAGCCTCGACACGCGGCTGGGCTCGCTCAACCTCAAGATCCCCAAGCTGCGCACCGGCAGCTACTTCCCCGGCTTCCTGGAGCCAAGGAAGACCGTC
GAGAAGGCGCTGGTGGCGGTGATCCAGGAGGCGTGGATCGCAGGCGTGTCGACTCGGCGCGTGGACGACCTGGTGCAGGCCATGGGGCTGTCGGGCATCT
CGAAGTCCTCCGTGTCGAAGCTGTGCAAGGACATCGATGAGCGGGTGAACGCCTTCCTCAAGCGCCCGCTGTCCGGCGCATGGCCCTATCTCTGGCTCGA
CGCCACCTACCTCAAGGTGCGTGAGGGCGGGCGCATCGTCTCCGTAGCCGCCATAGTCGCCGTGGCGGTGGACACCGAAGGTCGACGCGAGATCATCGGG
CTGCACATCGGCCCGTCCGAGGCCGAGGTGTTCTGGACCGATTTCCTGCGCGACCTGGTCAAGCGCGGGCTGTCAGGTGTGCAACTTGTCATCTCGGACG
CGCACGAGGGGCTTAAAGCCGCCATATGCCGCGTCCTGAAGGCGACCTGGCAAAGGTGCCGGGTTCATTGGACCCGCAACGCGCTCGCTTATGTGCCGCG
CGCTCAGCAGACCATGGTCGCCGCCGGCTTGCGCCACGCCTTCCAGCAGCCCGACCAGGAGGCAGCCCGAGCCGCGCTCCACCATCTGGCTGAGCAGCTT
CACAACCGTTGGCCGAAGCTCAAAGGCTTCATCGATGCCACGAGCGAGGACGTGCTCGCCTACCTGACGTTCCCGCTGCAGCACCGAACGAAGTTGCACT
CCACAAACCCGCTGGAGCGTCTCAACAAGGAGATCAAGCGACGCGCCGACGTGGTGGGCATCTTCCCCAACACCGACTCGATCCAGCGTCTGATCGGAGC
CGTGCTGTTGGAGGCCAACGACGAATGGCAGCTCCAGCACCGCTACATGCAGATCGAGGGCATGGCTGGCTTCACCCCGAGCCTGCTCGACGACACCCTC
ACAGCAATTCCACCTCAGGCCGCCTGACCGATGGCCGCTCCAACTCCACCCCGAATTTCCACCTCATTGACGGACACGACC
CCGAACCGGGAGCACCCGATGACCGACGACAGAGTGGCACTGATCGAGGCTTTTCAGAAGGCTGACGACGGCAACTTCCTGCGCAGCCTGGCCGAGACGG
TCCTGCAGATCCTGATGGAGGCCGATGTGGACGGTCTGATCGGCGCGGGTCGTTACGAGCGGTCTGGCGAGCGCAGCACCTACCGCAACGGGTATCGCGA
ACGCAGCCTCGACACGCGGCTGGGCTCGCTCAACCTCAAGATCCCCAAGCTGCGCACCGGCAGCTACTTCCCCGGCTTCCTGGAGCCAAGGAAGACCGTC
GAGAAGGCGCTGGTGGCGGTGATCCAGGAGGCGTGGATCGCAGGCGTGTCGACTCGGCGCGTGGACGACCTGGTGCAGGCCATGGGGCTGTCGGGCATCT
CGAAGTCCTCCGTGTCGAAGCTGTGCAAGGACATCGATGAGCGGGTGAACGCCTTCCTCAAGCGCCCGCTGTCCGGCGCATGGCCCTATCTCTGGCTCGA
CGCCACCTACCTCAAGGTGCGTGAGGGCGGGCGCATCGTCTCCGTAGCCGCCATAGTCGCCGTGGCGGTGGACACCGAAGGTCGACGCGAGATCATCGGG
CTGCACATCGGCCCGTCCGAGGCCGAGGTGTTCTGGACCGATTTCCTGCGCGACCTGGTCAAGCGCGGGCTGTCAGGTGTGCAACTTGTCATCTCGGACG
CGCACGAGGGGCTTAAAGCCGCCATATGCCGCGTCCTGAAGGCGACCTGGCAAAGGTGCCGGGTTCATTGGACCCGCAACGCGCTCGCTTATGTGCCGCG
CGCTCAGCAGACCATGGTCGCCGCCGGCTTGCGCCACGCCTTCCAGCAGCCCGACCAGGAGGCAGCCCGAGCCGCGCTCCACCATCTGGCTGAGCAGCTT
CACAACCGTTGGCCGAAGCTCAAAGGCTTCATCGATGCCACGAGCGAGGACGTGCTCGCCTACCTGACGTTCCCGCTGCAGCACCGAACGAAGTTGCACT
CCACAAACCCGCTGGAGCGTCTCAACAAGGAGATCAAGCGACGCGCCGACGTGGTGGGCATCTTCCCCAACACCGACTCGATCCAGCGTCTGATCGGAGC
CGTGCTGTTGGAGGCCAACGACGAATGGCAGCTCCAGCACCGCTACATGCAGATCGAGGGCATGGCTGGCTTCACCCCGAGCCTGCTCGACGACACCCTC
ACAGCAATTCCACCTCAGGCCGCCTGACCGATGGCCGCTCCAACTCCACCCCGAATTTCCACCTCATTGACGGACACGACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1209 bp | 402 aa | 55 | 1263 | + | No |
Chemistry : DDE
ORF sequence :
MTDDRVALIEAFQKADDGNFLRSLAETVLQILMEADVDGLIGAGRYERSGERSTYRNGYRERSLDTRLGSLNLKIPKLRTGSYFPGFLEPRKTVEKALVA
VIQEAWIAGVSTRRVDDLVQAMGLSGISKSSVSKLCKDIDERVNAFLKRPLSGAWPYLWLDATYLKVREGGRIVSVAAIVAVAVDTEGRREIIGLHIGPS
EAEVFWTDFLRDLVKRGLSGVQLVISDAHEGLKAAICRVLKATWQRCRVHWTRNALAYVPRAQQTMVAAGLRHAFQQPDQEAARAALHHLAEQLHNRWPK
LKGFIDATSEDVLAYLTFPLQHRTKLHSTNPLERLNKEIKRRADVVGIFPNTDSIQRLIGAVLLEANDEWQLQHRYMQIEGMAGFTPSLLDDTLTAIPPQ
AA
VIQEAWIAGVSTRRVDDLVQAMGLSGISKSSVSKLCKDIDERVNAFLKRPLSGAWPYLWLDATYLKVREGGRIVSVAAIVAVAVDTEGRREIIGLHIGPS
EAEVFWTDFLRDLVKRGLSGVQLVISDAHEGLKAAICRVLKATWQRCRVHWTRNALAYVPRAQQTMVAAGLRHAFQQPDQEAARAALHHLAEQLHNRWPK
LKGFIDATSEDVLAYLTFPLQHRTKLHSTNPLERLNKEIKRRADVVGIFPNTDSIQRLIGAVLLEANDEWQLQHRYMQIEGMAGFTPSLLDDTLTAIPPQ
AA
Blast result :
Comments
ISMex14 is 83% aa similar to ISAli7.
References
[1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.