ISMdi20
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1056 bp
Ends
IR Length : 21/26
IRL : GAGCCGTGCCCTAGCGGGTTGAATCACTTGGGGTAGGCGATCTGGCTGGC
IRR : GAGCCGTGCCCTTTCAGGTTGGCTCATAGGCATCGTATCCAGCAGCGGTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCGCAGGTC | TA | GCGGATCCGG | 2 |
DNA sequence
GAGCCGTGCCCTAGCGGGTTGAATCACTTGGGGTAGGCGATCTGGCTGGCGTGTGATTCACTGGGTGGGCTTGTGATGGAGGATGCCATGCCTTCAGCCC
TGTCGGTCGATCTGCGCGAGCGTGTAGTCGCCGCGATTGAAGCGGGTGCATCGCGGCGTCAGGCGGCCAAACGCTTCGGTGTCGGGGCGGCCAGCGCGGT
CCGTTGGCATGAGCGCTTCCGACAGGACGGGCAGATCGCGCCCAAGCCCATGGGCGGCGACCGCAACTCGCAGCGTCTGGAAGCGCAGGCCGCCCTGATC
CTTCGGCTCAACGAAGAACACCCGCTCTTCTTCCTGCGGGAACTGCGCGACAGGCTCTTGGAACGCGGCGTGCGGGCAAGCACGAGCAGCCTGTCACGCT
TCTTCGCCCGGCATGGGATCACCCGTAAAAAGGGGCTGTCCACGCGGCCGAGCAGGAGCGGTCGGATGTGAGGGCCGCGCGCGAGGACTGGTTCTATCGC
CAACCCGACCTTGATCCGGATCGCCTGGTTTTTCTCGACGAGACGGCTGCCACTACGAAGATGGTCCGCCGCTATGGTCGTGCTCCGTGCGGTCAGCGCT
GCCGGATCGCCGTGCCCCACGGGCACTACAAGACCACCACCATCACCGCCGCCCTGCGCGCCACGGGACTGATTGCCAGGAGCGTCTTCGATGGAGCCAC
GAATGGTATGCGCTTCCGCGCCTACGTCACCGACACCCTGGCTCCGCTGCTGAGGCCCGGTGACACCGTCATCCTCGACAATCTGAACGCCCACAAGGTC
GCCGGGGTGCGCGAGGCCATCGAGGCGGTCGGCGCACGGGTTCTCTACCTCCCGCCCTACAGTCCCGACTTCAACCCGATCGAGCAGGTCTTTGCCAAGG
CCAAAGCCCTCCTCCGGACGGCTGCAGCACGGACCGCCGCCGACCTGACCACCGCCATCGAGGACGCGTTCGCCGCTGTCCGACCGGATGAGTGCCGAAA
CTACATCACCGCTGCTGGATACGATGCCTATGAGCCAACCTGAAAGGGCACGGCTC
TGTCGGTCGATCTGCGCGAGCGTGTAGTCGCCGCGATTGAAGCGGGTGCATCGCGGCGTCAGGCGGCCAAACGCTTCGGTGTCGGGGCGGCCAGCGCGGT
CCGTTGGCATGAGCGCTTCCGACAGGACGGGCAGATCGCGCCCAAGCCCATGGGCGGCGACCGCAACTCGCAGCGTCTGGAAGCGCAGGCCGCCCTGATC
CTTCGGCTCAACGAAGAACACCCGCTCTTCTTCCTGCGGGAACTGCGCGACAGGCTCTTGGAACGCGGCGTGCGGGCAAGCACGAGCAGCCTGTCACGCT
TCTTCGCCCGGCATGGGATCACCCGTAAAAAGGGGCTGTCCACGCGGCCGAGCAGGAGCGGTCGGATGTGAGGGCCGCGCGCGAGGACTGGTTCTATCGC
CAACCCGACCTTGATCCGGATCGCCTGGTTTTTCTCGACGAGACGGCTGCCACTACGAAGATGGTCCGCCGCTATGGTCGTGCTCCGTGCGGTCAGCGCT
GCCGGATCGCCGTGCCCCACGGGCACTACAAGACCACCACCATCACCGCCGCCCTGCGCGCCACGGGACTGATTGCCAGGAGCGTCTTCGATGGAGCCAC
GAATGGTATGCGCTTCCGCGCCTACGTCACCGACACCCTGGCTCCGCTGCTGAGGCCCGGTGACACCGTCATCCTCGACAATCTGAACGCCCACAAGGTC
GCCGGGGTGCGCGAGGCCATCGAGGCGGTCGGCGCACGGGTTCTCTACCTCCCGCCCTACAGTCCCGACTTCAACCCGATCGAGCAGGTCTTTGCCAAGG
CCAAAGCCCTCCTCCGGACGGCTGCAGCACGGACCGCCGCCGACCTGACCACCGCCATCGAGGACGCGTTCGCCGCTGTCCGACCGGATGAGTGCCGAAA
CTACATCACCGCTGCTGGATACGATGCCTATGAGCCAACCTGAAAGGGCACGGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 399 bp | 132 aa | 73 | 471 | + | No |
Description : First part of the transposase
ORF sequence :
VMEDAMPSALSVDLRERVVAAIEAGASRRQAAKRFGVGAASAVRWHERFRQDGQIAPKPMGGDRNSQRLEAQAALILRLNEEHPLFFLRELRDRLLERGV
RASTSSLSRFFARHGITRKKGLSTRPSRSGRM
RASTSSLSRFFARHGITRKKGLSTRPSRSGRM
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 615 bp | 204 aa | 429 | 1043 | + | No |
Description : Second part of the transposase
ORF sequence :
KGAVHAAEQERSDVRAAREDWFYRQPDLDPDRLVFLDETAATTKMVRRYGRAPCGQRCRIAVPHGHYKTTTITAALRATGLIARSVFDGATNGMRFRAYV
TDTLAPLLRPGDTVILDNLNAHKVAGVREAIEAVGARVLYLPPYSPDFNPIEQVFAKAKALLRTAAARTAADLTTAIEDAFAAVRPDECRNYITAAGYDA
YEPT
TDTLAPLLRPGDTVILDNLNAHKVAGVREAIEAVGARVLYLPPYSPDFNPIEQVFAKAKALLRTAAARTAADLTTAIEDAFAAVRPDECRNYITAAGYDA
YEPT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 971 bp | 323 aa | 73 | 1043 | + | Yes |
Chemistry : DDE
ORF sequence :
VMEDAMPSALSVDLRERVVAAIEAGASRRQAAKRFGVGAASAVRWHERFRQDGQIAPKPMGGDRNSQRLEAQAALILRLNEEHPLFFLRELRDRLLERGV
RASTSSLSRFFARHGITRKKGAVHAAEQERSDVRAAREDWFYRQPDLDPDRLVFLDETAATTKMVRRYGRAPCGQRCRIAVPHGHYKTTTITAALRATGL
IARSVFDGATNGMRFRAYVTDTLAPLLRPGDTVILDNLNAHKVAGVREAIEAVGARVLYLPPYSPDFNPIEQVFAKAKALLRTAAARTAADLTTAIEDAF
AAVRPDECRNYITAAGYDAYEPT
RASTSSLSRFFARHGITRKKGAVHAAEQERSDVRAAREDWFYRQPDLDPDRLVFLDETAATTKMVRRYGRAPCGQRCRIAVPHGHYKTTTITAALRATGL
IARSVFDGATNGMRFRAYVTDTLAPLLRPGDTVILDNLNAHKVAGVREAIEAVGARVLYLPPYSPDFNPIEQVFAKAKALLRTAAARTAADLTTAIEDAF
AAVRPDECRNYITAAGYDAYEPT
Blast result :
Comments
ISMdi20 is 76% aa similar to ISMdi1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References