ISSoc15
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007775 | ND | Synechococcus sp. | Synechococcus sp. JA-3-3Ab |
DNA section
IS Length : 1016 bp
Ends
IR Length : 19/27
IRL : CAGCCTTTTAATTTAAAATAGGGTACAGGTATTCAAGAGGAAGGAGAGAA
IRR : CAGCCTGTTAAGGGCTCAAGGGGTACAATAGCAGCACTTAGCAAACCAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCAAGGGCT | TA | ATATCTCGCT | 2 |
DNA sequence
CAGCCTTTTAATTTAAAATAGGGTACAGGTATTCAAGAGGAAGGAGAGAAAGGAATGCCGACTCATTCTTTGGATTTGCGGCAAAGAGTTGTAGCAGCCT
ACCAGGCAGGTAACACCTCCATCCGCCAGGTAGCTAAACGCTTCATGGTGACCAAAAGAACAGTACACCGCTGGGTGCGTCAGTACCAACAAACTCAAGA
TTTAGCCCCTAAGAAAGCAGGCACCAAGCGAGTGGGCATTTTGGAACAACATCGGCAGGAAGTGATGGCAATTATTACAGAACACCCAGACTTCTACCTG
TGGCAGTATCAAGAACTGTTGCGCGAGCGCTTAGGAATCAATGTAAGCATCGTCACGATACATAATTTCTTGAAAAAGCAAGGAATGACTCTAAAAAAAA
GACCTACCGCAGTGCAAAAGTCAAAGAAGAGGAGGTGCAAAGGGAACGACTAGCTTATAGTCAAGAAGTCAGGAATATTCCAGCGGAGGATTTGATTGCC
ATCGACCAGACGGGAGTCTGGGAGGGAATGGAGCGGAGAGTATCTCGGAGTTTACGTGGTCAAAGGGCTTATCATTATCGTCAGAGATACAAGGGTGAAA
AGTATACGGTTATTGGAGCTATTTCCTTGAGAGGTGTAGTTGGCTGTCGTGTCATCAAGGGTGGGATGAAGAAAGGAGATTTTTTGGAGTTTTTGAGAAG
CGAGCTATGTCCGAAGCTAGATGCGAGGAAGGTTGTGATTATGGACAATTTAAATATCCACAAGAGTCGGGAAGTTGAGGAATTGATTAGGGGGACAGGA
GCACGAATCCTATACCTGCCTGTGTATGCGCCGGAGTTGAATCCCATTGAGATGATGTGGTCGGTGTTGAAGCATTTTATTCGGCAGCTTTGCAGAATTG
GGAAATATAGCATGGAGCAGATAGTGAAGACTTCTTTACTACTGATCAATCCATCCTCCTTCCGAAGTTGGTTTGCTAAGTGCTGCTATTGTACCCCTTG
AGCCCTTAACAGGCTG
ACCAGGCAGGTAACACCTCCATCCGCCAGGTAGCTAAACGCTTCATGGTGACCAAAAGAACAGTACACCGCTGGGTGCGTCAGTACCAACAAACTCAAGA
TTTAGCCCCTAAGAAAGCAGGCACCAAGCGAGTGGGCATTTTGGAACAACATCGGCAGGAAGTGATGGCAATTATTACAGAACACCCAGACTTCTACCTG
TGGCAGTATCAAGAACTGTTGCGCGAGCGCTTAGGAATCAATGTAAGCATCGTCACGATACATAATTTCTTGAAAAAGCAAGGAATGACTCTAAAAAAAA
GACCTACCGCAGTGCAAAAGTCAAAGAAGAGGAGGTGCAAAGGGAACGACTAGCTTATAGTCAAGAAGTCAGGAATATTCCAGCGGAGGATTTGATTGCC
ATCGACCAGACGGGAGTCTGGGAGGGAATGGAGCGGAGAGTATCTCGGAGTTTACGTGGTCAAAGGGCTTATCATTATCGTCAGAGATACAAGGGTGAAA
AGTATACGGTTATTGGAGCTATTTCCTTGAGAGGTGTAGTTGGCTGTCGTGTCATCAAGGGTGGGATGAAGAAAGGAGATTTTTTGGAGTTTTTGAGAAG
CGAGCTATGTCCGAAGCTAGATGCGAGGAAGGTTGTGATTATGGACAATTTAAATATCCACAAGAGTCGGGAAGTTGAGGAATTGATTAGGGGGACAGGA
GCACGAATCCTATACCTGCCTGTGTATGCGCCGGAGTTGAATCCCATTGAGATGATGTGGTCGGTGTTGAAGCATTTTATTCGGCAGCTTTGCAGAATTG
GGAAATATAGCATGGAGCAGATAGTGAAGACTTCTTTACTACTGATCAATCCATCCTCCTTCCGAAGTTGGTTTGCTAAGTGCTGCTATTGTACCCCTTG
AGCCCTTAACAGGCTG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 399 bp | 132 aa | 55 | 453 | + | No |
Description :
ORF sequence :
MPTHSLDLRQRVVAAYQAGNTSIRQVAKRFMVTKRTVHRWVRQYQQTQDLAPKKAGTKRVGILEQHRQEVMAIITEHPDFYLWQYQELLRERLGINVSIV
TIHNFLKKQGMTLKKRPTAVQKSKKRRCKGND
TIHNFLKKQGMTLKKRPTAVQKSKKRRCKGND
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 636 bp | 211 aa | 366 | 1001 | + | No |
Description :
ORF sequence :
FLEKARNDSKKKTYRSAKVKEEEVQRERLAYSQEVRNIPAEDLIAIDQTGVWEGMERRVSRSLRGQRAYHYRQRYKGEKYTVIGAISLRGVVGCRVIKGG
MKKGDFLEFLRSELCPKLDARKVVIMDNLNIHKSREVEELIRGTGARILYLPVYAPELNPIEMMWSVLKHFIRQLCRIGKYSMEQIVKTSLLLINPSSFR
SWFAKCCYCTP
MKKGDFLEFLRSELCPKLDARKVVIMDNLNIHKSREVEELIRGTGARILYLPVYAPELNPIEMMWSVLKHFIRQLCRIGKYSMEQIVKTSLLLINPSSFR
SWFAKCCYCTP
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 947 bp | 315 aa | 55 | 1001 | + | Yes |
Description :
ORF sequence :
MPTHSLDLRQRVVAAYQAGNTSIRQVAKRFMVTKRTVHRWVRQYQQTQDLAPKKAGTKRVGILEQHRQEVMAIITEHPDFYLWQYQELLRERLGINVSIV
TIHNFLKKQGMTLKKKTYRSAKVKEEEVQRERLAYSQEVRNIPAEDLIAIDQTGVWEGMERRVSRSLRGQRAYHYRQRYKGEKYTVIGAISLRGVVGCRV
IKGGMKKGDFLEFLRSELCPKLDARKVVIMDNLNIHKSREVEELIRGTGARILYLPVYAPELNPIEMMWSVLKHFIRQLCRIGKYSMEQIVKTSLLLINP
SSFRSWFAKCCYCTP
TIHNFLKKQGMTLKKKTYRSAKVKEEEVQRERLAYSQEVRNIPAEDLIAIDQTGVWEGMERRVSRSLRGQRAYHYRQRYKGEKYTVIGAISLRGVVGCRV
IKGGMKKGDFLEFLRSELCPKLDARKVVIMDNLNIHKSREVEELIRGTGARILYLPVYAPELNPIEMMWSVLKHFIRQLCRIGKYSMEQIVKTSLLLINP
SSFRSWFAKCCYCTP
Blast result :
Comments
ISSoc15 is 99% aa similar to ISSoc4.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
SSoc15 transposase was obtained by a frameshift ( 8A motif ). At the same position in ISSoc4, there is an 9A motif. So it is possible that one of the two IS is the result of a ponctual mutation at this position.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
SSoc15 transposase was obtained by a frameshift ( 8A motif ). At the same position in ISSoc4, there is an 9A motif. So it is possible that one of the two IS is the result of a ponctual mutation at this position.
References
1]Bhaya,D., Grossman,A.R., Steunou,A.S., Khuri,N., Cohan,F.M., Hamamura,N., Melendrez,M.C., Bateson,M.M., Ward,D.M. and Heidelberg,J.F. (2007) ISME J 1 (8), 703-713.
2]Allewalt,J.P., Bateson,M.M., Revsbech,N.P., Slack,K. and Ward,D.M. (2006) Appl. Environ. Microbiol. 72 (1), 544-550.
3]Heidelberg,J. (2005) Direct submission GenBank.
4]ISfinder annotation (2009)
2]Allewalt,J.P., Bateson,M.M., Revsbech,N.P., Slack,K. and Ward,D.M. (2006) Appl. Environ. Microbiol. 72 (1), 544-550.
3]Heidelberg,J. (2005) Direct submission GenBank.
4]ISfinder annotation (2009)