ISRosp5

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AAYC01000001 ND Roseobacter sp.
Roseobacter sp. SK209-2-6
DNA section
IS Length : 1217 bp

Ends


IR Length : 19/25

IRL : TGATCCTCCCCCACTGAAGTGATCCGCCCGTATAGTTAGGAAACGGAGGA
IRR : TGATCAGCCCCATCGAAGTGGTCCAGTGTGAGTGTTAGTGCATGATCGGC

Insertion site


Left flankDirect repeatRight flankDR Length
TTTCGAATACGTGGGAAAAC0

DNA sequence

TGATCCTCCCCCACTGAAGTGATCCGCCCGTATAGTTAGGAAACGGAGGATCAGATATGGGAATCAAGCGACACAAGCCCGAAGAGATTGTCACGAAGTT
ACGGCAGGTTGAGGTGCTTTGCGGCCAAGGGATGCCGCGCGTAGACGCCATTCGACAGGTGCAGATTACAGAACAAACCTTCTATCTCTGGCGCAAGCAA
TATGGCGGTATGGGAACCGACCAACTGAAGGAACTAAAGCGACTTCAGAAGGAGAATGACCGGCTGCGTCGGGCAGTTTCTGATCTGACATTGGACAAGC
TCATTCTGTCGGAAGCTGCACGGGGAAACTTCTGAGCCCCTCGCGCCGCCGTAACTGCATTGATCATGTGCGACGCCAGTTCCACGTCTCAGAGCGTCGC
GCCTGCCGTGTTCTGGGACAGCACCGTTCCACACAGAGGCGGGTGCCTGTTGGACGTGCTGACGAAGACCGGTTGGTGTCTGACATGATCGAACTGACAC
GCCAATATGGTCGATACGGTTACCGTCGGATAGCGGCTCTTCTGAGAGACGCCGGCTGGCAAATCAACGACAAACGTGTTGAGCGCCTATGGCGGCGTGA
GGGGCTGAAAGTTCCAATGAAACAACCGAAGAAAGGACGGCTCTGGCTGAACGACGGGTCGTGTGTTCGACTCCGGCCCGAATATCGTGACCATGTCTGG
TCCTATGACTTCGTGCACCATCGAACGGATGACGGGAAAGCGTTCCGGACACTGAACATTCTGGATGAACACAGCCGGGAATGCCTGGCGATCCGGGTGA
AGAGGCGCTTGAACTCGGCAGAAGTTATTGACGCGCTGACAGATCTTTTCATCCTGCGTGGCGTTCCTGCTTTCATCAGATCAGATAATGGCCCAGAGTT
CATTGCTCAGGCCGTTCGAGATTGGATCGCAGCGGTCGGTGCCCAAACGGCCTACATTGAACCAGGATCACCTTGGGAGAACGGATACTGTGAAAGCTTC
AACGCGCGCTTCAGAGACGAGCTGCTGAATGGAGAGGTATTTTACAGCCTGCGTGAAGCCCAGATCCTGATTGAACAATGGAGAAATCACTACAACACAA
AACGGCCGCATAGTGCTCTGGGCTATCGCCCACCGGCTCCGGAAGCCATCGTTCCGGTGGAAACCAGGCCGATCATGCACTAACACTCACACTGGACCAC
TTCGATGGGGCTGATCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
279 bp92 aa57335+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MGIKRHKPEEIVTKLRQVEVLCGQGMPRVDAIRQVQITEQTFYLWRKQYGGMGTDQLKELKRLQKENDRLRRAVSDLTLDKLILSEAARGNF

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
900 bp299 aa2841183+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SDIGQAHSVGSCTGKLLSPSRRRNCIDHVRRQFHVSERRACRVLGQHRSTQRRVPVGRADEDRLVSDMIELTRQYGRYGYRRIAALLRDAGWQINDKRVE
RLWRREGLKVPMKQPKKGRLWLNDGSCVRLRPEYRDHVWSYDFVHHRTDDGKAFRTLNILDEHSRECLAIRVKRRLNSAEVIDALTDLFILRGVPAFIRS
DNGPEFIAQAVRDWIAAVGAQTAYIEPGSPWENGYCESFNARFRDELLNGEVFYSLREAQILIEQWRNHYNTKRPHSALGYRPPAPEAIVPVETRPIMH

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1127 bp375 aa571183+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGIKRHKPEEIVTKLRQVEVLCGQGMPRVDAIRQVQITEQTFYLWRKQYGGMGTDQLKELKRLQKENDRLRRAVSDLTLDKLILSEAARGKLLSPSRRRN
CIDHVRRQFHVSERRACRVLGQHRSTQRRVPVGRADEDRLVSDMIELTRQYGRYGYRRIAALLRDAGWQINDKRVERLWRREGLKVPMKQPKKGRLWLND
GSCVRLRPEYRDHVWSYDFVHHRTDDGKAFRTLNILDEHSRECLAIRVKRRLNSAEVIDALTDLFILRGVPAFIRSDNGPEFIAQAVRDWIAAVGAQTAY
IEPGSPWENGYCESFNARFRDELLNGEVFYSLREAQILIEQWRNHYNTKRPHSALGYRPPAPEAIVPVETRPIMH

 

Blast result :
Comments
Genome in progress.
ISRosp5 is 82% aa similar to ISMex5.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Ward,B., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2006) Direct submission GenBank.
2] ISfinder annotation (2009).