ISfinder Mirror Site

ISBsp3

Family IS3
Group IS150

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAXV01000003	ND	Bacillus sp.	Bacillus sp. B14905

DNA section

IS Length : 1500 bp

Ends

IR Length : 31/40

IRL : TGAACTGAACCCCGAATAGTGGACACTTTAAAAAGTGACCCTATTCGGGG
IRR : TGAACTGAACCCTATAAAGTAGACAGTTTAATAAAAGACCATTTGCTACA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACTTCCTTTT	TTC	AACGGGAAGT	3
GGATGCTCAC	CTT	ACTGGGAGTC	3
AGGCTCCAAT	TAA	TAGAGCAACT	3
AAGCCTAGTA	GCT	ATACAGTTAC	3
TAAGTATAGT	TTA	TACTCATGGG	3
GGCTCAAAAT	TA	ATTGAGTACC	2

DNA sequence

TGAACTGAACCCCGAATAGTGGACACTTTAAAAAGTGACCCTATTCGGGGTTTTTTGTTCATTTCACAAAAAAATCTCGTTATACTAAATATACGAAATT
AAGAACGGGAGAAATTAGCATGAGTAAAATCATTTTTAACGAACATCAGATACGACAAATAGAAGCGAATCGATTTGTAGCATCTGTATCAGATCGTTCT
ATTCAATATACAGCGGAATATAAAATCTTTGCGGTCAAAGAAAATCTAGCTGGTAAGGGACCTGTACAAATCTTTAAAGAAAACGGGTTTGATTTAGAAG
TGATTGGTGTGGAAAAAGCAAGGTCATCGATTAAACGCTGGAGAGCATCTTTTAAAACTCATGGAGAACAAGCATTTTCAGAGGAAAGACGCGGAAAAGG
AGGTTCTGGTCGACCAAAAAAAGAACACATGTCAGCTGAAAAGAAATTAGAAAAAGCAGAGGCACGTATTCGTTTACTTGAAGCTGAATTAACACTGTTA
AAAAAGCTAGACGAGATGGAAAGGCAGGCAAAGAAACGGCTATTTTAATACCAAGGGAAAAGTATCAAGCAATTAATGATACCGTTCGGCTGTATCAGTT
GAAAAATATGACACGTTATTTATGTGATTTGGCAAATGTAAGCCCCAGTGGTTATTATAAATGGCTCCAAAATAGTGAGAAACAAGCGATGCGAGAAGAA
GCAGATTTTCAGGACTACGTCCTACTAAAAACGATTTATGATGCGTCAAAAGGAAAAGTTGGTTATCGGGGTCTTTACATGGCACTAGAAGAACTACTTG
AAATCCCGATGAATCATAAAAAAATTCTACGATTAATGCGTAAATTCAATCTATATTCGAAAGTAAGACGTGCAAACCCTTATCGAAATCTTGCCAAAGC
TACAGAAATCCATCGTCAAGTACCTAATCATCTAAATCGTAATTTTCATCAAACAGAGCCAGGCAAAGTGTTTTTAACAGATATTACGTATTTACAATAT
CGTGGCGGACAAACAGCGTATCTATCGTGTATAAAGGATGTTGCAACACGAGAAATCGTTGCATATGAGGTATCGACAAATTTACGCATGGAGTTTGTTT
ATCGTACGCTAAAAAAATTAGAAGAGCATATCAATGACAATTTACATCCTGAAGCGATGATTCATTCAGATCAAGGTTTCCATTATACACACCCAGCATT
TCAAAAATGCGTCAAAGAAATGGGATTACTTCAGTCAATGTCTCGCCGAGGAAACTGCCTAGATAATTCGCCAATGGAGTCATTCTTTGGGCATTTTAAA
GATGAAATAGAATATAAAGAAGCTGGAAGCTTATCAGAATTAAAATATATGATAGATGATTATATGGAGCACTACAACAATACAAGAAAGCAATGGACGT
TAAAAAAAATGACTCCGGCAGCATACCGAAGTCATTTAATCGCTGTTTAATGTAGCAAATGGTCTTTTATTAAACTGTCTACTTTATAGGGTTCAGTTCA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
429 bp	142 aa	120	548	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSKIIFNEHQIRQIEANRFVASVSDRSIQYTAEYKIFAVKENLAGKGPVQIFKENGFDLEVIGVEKARSSIKRWRASFKTHGEQAFSEERRGKGGSGRPK
KEHMSAEKKLEKAEARIRLLEAELTLLKKLDEMERQAKKRLF

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
963 bp	320 aa	488	1450	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

INTVKKARRDGKAGKETAILIPREKYQAINDTVRLYQLKNMTRYLCDLANVSPSGYYKWLQNSEKQAMREEADFQDYVLLKTIYDASKGKVGYRGLYMAL
EELLEIPMNHKKILRLMRKFNLYSKVRRANPYRNLAKATEIHRQVPNHLNRNFHQTEPGKVFLTDITYLQYRGGQTAYLSCIKDVATREIVAYEVSTNLR
MEFVYRTLKKLEEHINDNLHPEAMIHSDQGFHYTHPAFQKCVKEMGLLQSMSRRGNCLDNSPMESFFGHFKDEIEYKEAGSLSELKYMIDDYMEHYNNTR
KQWTLKKMTPAAYRSHLIAV

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1331 bp	443 aa	120	1450	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSKIIFNEHQIRQIEANRFVASVSDRSIQYTAEYKIFAVKENLAGKGPVQIFKENGFDLEVIGVEKARSSIKRWRASFKTHGEQAFSEERRGKGGSGRPK
KEHMSAEKKLEKAEARIRLLEAELTLLKKARRDGKAGKETAILIPREKYQAINDTVRLYQLKNMTRYLCDLANVSPSGYYKWLQNSEKQAMREEADFQDY
VLLKTIYDASKGKVGYRGLYMALEELLEIPMNHKKILRLMRKFNLYSKVRRANPYRNLAKATEIHRQVPNHLNRNFHQTEPGKVFLTDITYLQYRGGQTA
YLSCIKDVATREIVAYEVSTNLRMEFVYRTLKKLEEHINDNLHPEAMIHSDQGFHYTHPAFQKCVKEMGLLQSMSRRGNCLDNSPMESFFGHFKDEIEYK
EAGSLSELKYMIDDYMEHYNNTRKQWTLKKMTPAAYRSHLIAV

Blast result :

Comments

ISBsp3 is 64% ISClte1.

References

1] Edwards,R., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2006) Direct submission GenBank.
2] ISfinder annotation (2009).