ISfinder Mirror Site

ISAcma32

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017 plasmid

DNA section

IS Length : 1161 bp

Ends

IR Length : 17/20

IRL : CTCCCTTCCCGCTGTAAAATGGGGAAAAGAGCAAATAGTCACATTGAATC
IRR : CTCCCTTCCCGCCATAAGATTATGGAACAAGTGAAAATATAAAATTCAGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGTTGCCCTT	TA	AATAGTCCTT	2
AACGTTCACT	TA	ATTATGAAAG	2
CAACCATAGC	TTA	TTTCCTAAAC	3
TCACCTCAAA	TTA	GTCAGCAACT	3
TCAGCTCTGA	TTA	TTACATGTGT	3

DNA sequence

CTCCCTTCCCGCTGTAAAATGGGGAAAAGAGCAAATAGTCACATTGAATCGAACACAAGCCTTTCTGGACAAGCCTTTTGATGCGGATGAGTGGCAGAAA
TTCTACTACCGCAATCAACAACACTACATTCGACAGCGCTTGACCGCGATCAAATTGCTGAATGAAGGACACAGTCGTACCCAAGTGAGCGAACAAGTGG
GGTGTAGCTATGATACGTTAACGCGTTGGATGGACAAATATCTCGATGGTGGACTCCAGGGCTTGGTCCAACCCATTCGACATCAAAAACCTAGTCGGTT
GAGCCCAGAGGAACAACAACAACTCAAAGAGATGGTTCTGACGCAACGTCCGACAGACTATGGTATTGACCGCAATATGTGGACAGGTGCAATTTTAGCC
GTTGTGATTGAGCAGCGTTTCGAGGTGCAGCTGAAAGATTCGCGTATCTACGAATTGCTATCAGAGTTGGGGTTATCTTACCAACGCGCCCATCGTGACT
ATGCGAATGCTGACCTGAACGCCCAGAAAGAGTGGGTGGCGGCTGTTAAAAAAACTGCAATCACGACAATCGGATGAGCGCATCTTGTTCTTTGACGAAT
TTGCGGTGTCTGAGCGTCCTAGGTTGTTCTATGGCTGGGCAGAACGCAACACTCGTCCACAGGTCAAGAGTAATGAGCGGGCTCGCAACAAGCTCAATGG
GTTACTGTGCGTCGATGCCCACAGTGGCGAAGAGTATTTCGCTCTGAGCCCACAGGCTAAAACAGAAGATATCTCTGAGTACTTTGCAGACCTATGTCTG
GATAGCGTTGAGCTGGGCTACACTCAACTGTGCCTTATTCTCGATAACAATCCGACCCATAAGGGCAAGATGCGCTCCCAACTCGCTATCCATCTAGAGC
AGATGGGGCTCACCCAATCGATTCAAGTTGAGTTTTTATATTTGCCGTCCTACTCTCCCAAGTTGAACTTGGTTGAATATGTGATTCACTTACTACGCTT
ACGATGTCTGCACCATCTCCCTCTTGGAACGACCCTGACACAGATCAAACAACAGCTCCATGAGTTCTTTGCTGCTAACCAGTTTCTATCGGCTGAACAA
GTTCAAAACAGCCTGAATTTTATATTTTCACTTGTTCCATAATCTTATGGCGGGAAGGGAG

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
534 bp	177 aa	44	577	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MNRTQAFLDKPFDADEWQKFYYRNQQHYIRQRLTAIKLLNEGHSRTQVSEQVGCSYDTLTRWMDKYLDGGLQGLVQPIRHQKPSRLSPEEQQQLKEMVLT
QRPTDYGIDRNMWTGAILAVVIEQRFEVQLKDSRIYELLSELGLSYQRAHRDYANADLNAQKEWVAAVKKTAITTIG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
624 bp	207 aa	519	1142	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TPRKSGWRLLKKLQSRQSDERILFFFFDEFAVSERPRLFYGWAERNTRPQVKSNERARNKLNGLLCVDAHSGEEYFALSPQAKTEDISEYFADLCLDSVE
LGYTQLCLILDNNPTHKGKMRSQLAIHLEQMGLTQSIQVEFLYLPSYSPKLNLVEYVIHLLRLRCLHHLPLGTTLTQIKQQLHEFFAANQFLSAEQVQNS
LNFIFSLVP

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1099 bp	365 aa	44	1142	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNRTQAFLDKPFDADEWQKFYYRNQQHYIRQRLTAIKLLNEGHSRTQVSEQVGCSYDTLTRWMDKYLDGGLQGLVQPIRHQKPSRLSPEEQQQLKEMVLT
QRPTDYGIDRNMWTGAILAVVIEQRFEVQLKDSRIYELLSELGLSYQRAHRDYANADLNAQKEWVAAVKKLQSRQSDERILFFDEFAVSERPRLFYGWAE
RNTRPQVKSNERARNKLNGLLCVDAHSGEEYFALSPQAKTEDISEYFADLCLDSVELGYTQLCLILDNNPTHKGKMRSQLAIHLEQMGLTQSIQVEFLYL
PSYSPKLNLVEYVIHLLRLRCLHHLPLGTTLTQIKQQLHEFFAANQFLSAEQVQNSLNFIFSLVP

Blast result :

Comments

ISAcma32 is 42% aa similar to ISSpn2.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible +1 frameshift.

References

1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)