ISCth8
- Family IS110
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009012 | ND | Clostridium thermocellum | Clostridium thermocellum ATCC 27405 |
DNA section
IS Length : 1907 bp
Ends
Left end : TCCTTGTACAATTAATATTGTTATTTCATCAGTGACTTTTGCCACTGTTCGACCCTGTTAACATAATGTCATATTTGTTGCTTTTCTAAACATCAAGCAC
Right end : AGGTGGTCTTGTCGTTGTGCCTTTTTTCGCTGTCAGGAGTTTTAGAAAATTCTTAAAATTTTTTCTTAAAAACTCTTGACTTTAATTAGCTGGTCTTTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATGAGAATTTTATCTTTCA | TATTTTTTATTTATTATCTC | 0 | |
| CCTTCATATGTTTATTTAAC | GTGATTTACAAAAAATTATA | 0 | |
| TCCACCCATATCTTTATCAC | ATATCTGTATTTGTTGCTTG | 0 | |
| AATGTATTCATATAATATCC | TTTTATCAATGGGTTAATAC | 0 |
DNA sequence
TCCTTGTACAATTAATATTGTTATTTCATCAGTGACTTTTGCCACTGTTCGACCCTGTTAACATAATGTCATATTTGTTGCTTTTCTAAACATCAAGCAC
TTCTATGTGCCATCCCCTAGGGGATGGCGGTGGCTTTCAAATCCTGCCGCCTGCGGTTCCCCATCTATGACCGCTTGCTAGCTTTCGTCTGGCCTGAACC
TTGTCAATCATGTATAATATTTTCCGGACTTGGAGAGTGTTTGTAATCACTCCTGGGACGGACCTTATTGGGCCGACTACCCGTAAGAAAGAGTATTTAT
CCATTCCGGTAAGTCTAAATGATTTGGCTGGTTGAGGCCAGCATTTCAGCTGGCTCCTCGTGTCACACGCAAGGTTGTTTACTTACATGAGAAGGAATGG
AGTCTTTTAAGTCCATTAATCTTATAAAGGAGGCATTTTTATGAATTTTAGACCCATCGCCGGAATCGATGTCGGCAAGTTCTTCAGTGAGATGGCAATT
CTTTCTCCATCCAATGAAGTAATTGCCCGCATGAAGATCCGCCATGATTCCAGTACTGACGTTGAAAGAGCCGTTGAATTACTGAAAAAAACGGAAAAGG
ACTTTGATTCTAGGCCTTTCGTCGTCATGGAATCCACTGGGCACTATCACAAAATCCTTTTCCATTCACTTTATAAAGCTGGATTTGAGGTTTCTGTCAT
AAACCCCATCCAAACTGATTCTATCAAAAATATTGGAATAAGGAAAGTGAAAAATGATAAAGTGGATGCCCGGAAAATTGCTCTGCTATACAGATTTCAG
GAGCTTAAAACTACCAATATCCCCGACGAGGATATTGAATGTCTGCGAAGCCTTTGCCGCCAGTACTACAAGCTCTCTGACGAACTTACTGCTTACAAAA
ACAGGCTTATGGGTATTGTTGACCAACTCATGCTAAACTTCAAGGATGTATTCCCTAATATCTTTTCAAAGGCTGCTCTTGCAGTATTGGAGAAATATCC
TGCACCTGCGCATATTCTTAAAGCGAACAGAAACAAGTTGATTGCACTGATACAGAAGAATTCCCGCAGAAGCCTTAAATGGGCAACTGCAAAGTATGAG
CTTTTGAATTCCAAGGCCAAAGAATTTGCACCTTTAAGCATTAGTAACTCTTCAAATGTTGCCATGCTTGGTGTGTATATCTCTATGATTAAAACCTTGG
AAGAAAACCTTGAGAAAGTCCTCAAAGCCATTCGTTCATTGATTATTGAAGATATGGCAAAGGACATGCCCATGCTGGCACTGACTCTCGAGCTTCTACA
AAGCATTCCAGGTATAGGACTTATCTCTGCTGTTACCATTCTGGCTGAAATTGGCGACTTTTCAGCCTTTTCAAAGCCAGGCAAGCTAGTTGCTTATTTC
GGTATTGACCCCTCTGTAATGCAGTCCGGAGAGTTTACCGGCACACAAAACAAGATGTCAAAAAGGGGGTCAAGACTGCTTCGCAGAGTACTTTTCACAA
TTGCTCTTGCTAATATCCGCACCAAGCGGGACAAAACAGCTTGCAACCCTGTACTGATGGAATATTACAAAAACAAATGCCAGAGCAAGCCCAAGAAAGT
AGCTTTGGGGGCTGTTATGCGTAAGCTTGTTAATTATATTTTTGCTGTTCTTAGGGATAGAAAGCCTTACGAATTACGTTCTCCCCAAGAGCACGCGCAA
ATGCTTGCAGCGAAGCACACAGCAGCTTAGTAGTACTTGTACTCGATGTTTAGTTTTCAAAAATCAATTTACTATTTCAGGCCAGCTATTTTATGTCTAC
TAATCTTAGGTGGTCTTGTCGTTGTGCCTTTTTTCGCTGTCAGGAGTTTTAGAAAATTCTTAAAATTTTTTCTTAAAAACTCTTGACTTTAATTAGCTGG
TCTTTCT
TTCTATGTGCCATCCCCTAGGGGATGGCGGTGGCTTTCAAATCCTGCCGCCTGCGGTTCCCCATCTATGACCGCTTGCTAGCTTTCGTCTGGCCTGAACC
TTGTCAATCATGTATAATATTTTCCGGACTTGGAGAGTGTTTGTAATCACTCCTGGGACGGACCTTATTGGGCCGACTACCCGTAAGAAAGAGTATTTAT
CCATTCCGGTAAGTCTAAATGATTTGGCTGGTTGAGGCCAGCATTTCAGCTGGCTCCTCGTGTCACACGCAAGGTTGTTTACTTACATGAGAAGGAATGG
AGTCTTTTAAGTCCATTAATCTTATAAAGGAGGCATTTTTATGAATTTTAGACCCATCGCCGGAATCGATGTCGGCAAGTTCTTCAGTGAGATGGCAATT
CTTTCTCCATCCAATGAAGTAATTGCCCGCATGAAGATCCGCCATGATTCCAGTACTGACGTTGAAAGAGCCGTTGAATTACTGAAAAAAACGGAAAAGG
ACTTTGATTCTAGGCCTTTCGTCGTCATGGAATCCACTGGGCACTATCACAAAATCCTTTTCCATTCACTTTATAAAGCTGGATTTGAGGTTTCTGTCAT
AAACCCCATCCAAACTGATTCTATCAAAAATATTGGAATAAGGAAAGTGAAAAATGATAAAGTGGATGCCCGGAAAATTGCTCTGCTATACAGATTTCAG
GAGCTTAAAACTACCAATATCCCCGACGAGGATATTGAATGTCTGCGAAGCCTTTGCCGCCAGTACTACAAGCTCTCTGACGAACTTACTGCTTACAAAA
ACAGGCTTATGGGTATTGTTGACCAACTCATGCTAAACTTCAAGGATGTATTCCCTAATATCTTTTCAAAGGCTGCTCTTGCAGTATTGGAGAAATATCC
TGCACCTGCGCATATTCTTAAAGCGAACAGAAACAAGTTGATTGCACTGATACAGAAGAATTCCCGCAGAAGCCTTAAATGGGCAACTGCAAAGTATGAG
CTTTTGAATTCCAAGGCCAAAGAATTTGCACCTTTAAGCATTAGTAACTCTTCAAATGTTGCCATGCTTGGTGTGTATATCTCTATGATTAAAACCTTGG
AAGAAAACCTTGAGAAAGTCCTCAAAGCCATTCGTTCATTGATTATTGAAGATATGGCAAAGGACATGCCCATGCTGGCACTGACTCTCGAGCTTCTACA
AAGCATTCCAGGTATAGGACTTATCTCTGCTGTTACCATTCTGGCTGAAATTGGCGACTTTTCAGCCTTTTCAAAGCCAGGCAAGCTAGTTGCTTATTTC
GGTATTGACCCCTCTGTAATGCAGTCCGGAGAGTTTACCGGCACACAAAACAAGATGTCAAAAAGGGGGTCAAGACTGCTTCGCAGAGTACTTTTCACAA
TTGCTCTTGCTAATATCCGCACCAAGCGGGACAAAACAGCTTGCAACCCTGTACTGATGGAATATTACAAAAACAAATGCCAGAGCAAGCCCAAGAAAGT
AGCTTTGGGGGCTGTTATGCGTAAGCTTGTTAATTATATTTTTGCTGTTCTTAGGGATAGAAAGCCTTACGAATTACGTTCTCCCCAAGAGCACGCGCAA
ATGCTTGCAGCGAAGCACACAGCAGCTTAGTAGTACTTGTACTCGATGTTTAGTTTTCAAAAATCAATTTACTATTTCAGGCCAGCTATTTTATGTCTAC
TAATCTTAGGTGGTCTTGTCGTTGTGCCTTTTTTCGCTGTCAGGAGTTTTAGAAAATTCTTAAAATTTTTTCTTAAAAACTCTTGACTTTAATTAGCTGG
TCTTTCT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1299 bp | 429 aa | 432 | 1730 | + | No |
Chemistry : DEDD
ORF sequence :
MNFRPIAGIDVGKFFSEMAILSPSNEVIARMKIRHDSSTDVERAVELLKKTEKDFDSRPFVVMESTGHYHKILFHSLYKAGFEVSVINPIQTDSIKNIGI
RKVKNDKVDARKIALLYRFQELKTTNIPDEDIECLRSLCRQYYKLSDELTAYKNRLMGIVDQLMLNFKDVFPNIFSKAALAVLEKYPAPAHILKANRNKL
IALIQKNSRRSLKWATAKYELLNSKAKEFAPLSISNSSNVAMLGVYISMIKTLEENLEKVLKAIRSLIIEDMAKDMPMLALTLELLQSIPGIGLISAVTI
LAEIGDFSAFSKPGKLVAYFGIDPSVMQSGEFTGTQNKMSKRGSRLLRRVLFTIALANIRTKRDKTACNPVLMEYYKNKCQSKPKKVALGAVMRKLVNYI
FAVLRDRKPYELRSPQEHAQMLAAKHTAA
RKVKNDKVDARKIALLYRFQELKTTNIPDEDIECLRSLCRQYYKLSDELTAYKNRLMGIVDQLMLNFKDVFPNIFSKAALAVLEKYPAPAHILKANRNKL
IALIQKNSRRSLKWATAKYELLNSKAKEFAPLSISNSSNVAMLGVYISMIKTLEENLEKVLKAIRSLIIEDMAKDMPMLALTLELLQSIPGIGLISAVTI
LAEIGDFSAFSKPGKLVAYFGIDPSVMQSGEFTGTQNKMSKRGSRLLRRVLFTIALANIRTKRDKTACNPVLMEYYKNKCQSKPKKVALGAVMRKLVNYI
FAVLRDRKPYELRSPQEHAQMLAAKHTAA
Blast result :
Comments
ISCth8 is 63% aa similar to ISDha10. There are 4 copies in the genome.
References
1] Miriam Land (2009) Direct submission
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.