ISCth6
- Family IS110
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009012 | ND | Clostridium thermocellum | Clostridium thermocellum ATCC 27405 |
DNA section
IS Length : 1395 bp
Ends
Left end : CTAACGCTCTTAAATTGAGGTTGCCGGTATGAGTGGTGGAGACATTGGAAAAGGAAAGGATAGATATTTATCATAAACGAAACACCTGCTTTGTTGGAAT
Right end : ATTCTGGAAGCAAAGCAATGGAAGATAATTCTTTTTTTCGTTGAGATATGGTAACAGGAATTATATAATAGATATAGTCCTTGTAGTGAGGGAACGCGTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGGTTCTTAGACTAACGCT | AGACTACACCGAAGACAACA | 0 | |
| AGAAATGTCCCTAATCGAAT | GTACTACAGGAAAACAAGCT | 0 | |
| AGAAATGTCCCTAATCGAAT | GTACTGTTCCTAATAAAGTA | 0 |
DNA sequence
CTAACGCTCTTAAATTGAGGTTGCCGGTATGAGTGGTGGAGACATTGGAAAAGGAAAGGATAGATATTTATCATAAACGAAACACCTGCTTTGTTGGAAT
TGATATGCACAAGGACGCACATTGTGCAGTTGTAATTGATTGTTGGATGAATAAACTGGGTGAGGTTAACTTTGAAAACAGGCCATCCAGATTCCCTGCA
TTCGTTGAGGATGTAAGGAAGATTTGCGGGATAAAGGAAATTGTATTCGGACTTGAAGATACCAGAGGCTTTGGCAGAAACCTTGCTGCCTATCTGGTGG
GCAGGAAGTTTGAAGTAAAGCACGTAAACCCTGCATATACAAGCGCTGTAAGGCTTGCAAACCCCATTATTTACAAGGATGACTCCTATGATGCCTATTG
TGTGGCAAGGGTGCTCAGGGATATGGTGGACACTCTGCAGGATGCCAAGCATGAGGATATATTCTGGACAATACGGCAAATGGTGAAAAGACGGGATTTG
ATTGTAAAAAGTAATGTGATGAACAAGAACCAGCTCCACAGCCAGCTTGCTTATAGCTACCCATCCTACAGGAAATTCTTTGCCATGATTGATTCCAAGA
GTGCCTTATGCTTCTGGGAGAACTACCCGTCACCGGAGTATATATGGAAAACAACACCAGAAGAAATATATCAGACGATAAAGCCTGTGCATCAGGCGCT
TAAAATACAGCGCATCCATGAGATTATATCCATGATTGAAAGGGATGGAGACACAAGAAAGGACTATCAGCCCGAAAGGGATTTTATTGTGAGAAACATC
GTGAAGGATATCAGACACAACAAGGAGTTGATTGCCGAAATTGACGATGAACTAAGAAAGCTGATACCTTTGACAGGCTATAAGCTACATACAATGCCGG
GAATCGACCTTGTTACAGAAGCACAGATAATATCTGAAATCGGAGATATTAACCGTTTCCCTGACTCAGACAAGCTGGCTCGGTTTATGGGCTTGGCACC
GGTGCAATTCAGCTCTGCCGGAAAGGGTAAAGACCAAAGATGCAGGAATGGCAACAGGGCACTAAATGCGATATTTCACTTTCTTGCAATCCAGATGGTA
GCAGTATCGGCCTCAGGAAAGCCAAGACACCCGGTATTCAGGGAGTATTTTGAGCAGAAGGTCAAAGAGGGCAAGAACAAGCCACAGGCGCTTGTATGCG
TGGCAAGGCGGCTTGTGAGGATAATCTACGGTATGATGAAAACCAAGACTGAATACAGGCCATATGAGAAGACTGACGACAAGAACTGATTTCATATTCT
GGAAGCAAAGCAATGGAAGATAATTCTTTTTTTCGTTGAGATATGGTAACAGGAATTATATAATAGATATAGTCCTTGTAGTGAGGGAACGCGTA
TGATATGCACAAGGACGCACATTGTGCAGTTGTAATTGATTGTTGGATGAATAAACTGGGTGAGGTTAACTTTGAAAACAGGCCATCCAGATTCCCTGCA
TTCGTTGAGGATGTAAGGAAGATTTGCGGGATAAAGGAAATTGTATTCGGACTTGAAGATACCAGAGGCTTTGGCAGAAACCTTGCTGCCTATCTGGTGG
GCAGGAAGTTTGAAGTAAAGCACGTAAACCCTGCATATACAAGCGCTGTAAGGCTTGCAAACCCCATTATTTACAAGGATGACTCCTATGATGCCTATTG
TGTGGCAAGGGTGCTCAGGGATATGGTGGACACTCTGCAGGATGCCAAGCATGAGGATATATTCTGGACAATACGGCAAATGGTGAAAAGACGGGATTTG
ATTGTAAAAAGTAATGTGATGAACAAGAACCAGCTCCACAGCCAGCTTGCTTATAGCTACCCATCCTACAGGAAATTCTTTGCCATGATTGATTCCAAGA
GTGCCTTATGCTTCTGGGAGAACTACCCGTCACCGGAGTATATATGGAAAACAACACCAGAAGAAATATATCAGACGATAAAGCCTGTGCATCAGGCGCT
TAAAATACAGCGCATCCATGAGATTATATCCATGATTGAAAGGGATGGAGACACAAGAAAGGACTATCAGCCCGAAAGGGATTTTATTGTGAGAAACATC
GTGAAGGATATCAGACACAACAAGGAGTTGATTGCCGAAATTGACGATGAACTAAGAAAGCTGATACCTTTGACAGGCTATAAGCTACATACAATGCCGG
GAATCGACCTTGTTACAGAAGCACAGATAATATCTGAAATCGGAGATATTAACCGTTTCCCTGACTCAGACAAGCTGGCTCGGTTTATGGGCTTGGCACC
GGTGCAATTCAGCTCTGCCGGAAAGGGTAAAGACCAAAGATGCAGGAATGGCAACAGGGCACTAAATGCGATATTTCACTTTCTTGCAATCCAGATGGTA
GCAGTATCGGCCTCAGGAAAGCCAAGACACCCGGTATTCAGGGAGTATTTTGAGCAGAAGGTCAAAGAGGGCAAGAACAAGCCACAGGCGCTTGTATGCG
TGGCAAGGCGGCTTGTGAGGATAATCTACGGTATGATGAAAACCAAGACTGAATACAGGCCATATGAGAAGACTGACGACAAGAACTGATTTCATATTCT
GGAAGCAAAGCAATGGAAGATAATTCTTTTTTTCGTTGAGATATGGTAACAGGAATTATATAATAGATATAGTCCTTGTAGTGAGGGAACGCGTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1257 bp | 418 aa | 33 | 1289 | + | No |
Chemistry : DEDD
ORF sequence :
MVETLEKERIDIYHKRNTCFVGIDMHKDAHCAVVIDCWMNKLGEVNFENRPSRFPAFVEDVRKICGIKEIVFGLEDTRGFGRNLAAYLVGRKFEVKHVNP
AYTSAVRLANPIIYKDDSYDAYCVARVLRDMVDTLQDAKHEDIFWTIRQMVKRRDLIVKSNVMNKNQLHSQLAYSYPSYRKFFAMIDSKSALCFWENYPS
PEYIWKTTPEEIYQTIKPVHQALKIQRIHEIISMIERDGDTRKDYQPERDFIVRNIVKDIRHNKELIAEIDDELRKLIPLTGYKLHTMPGIDLVTEAQII
SEIGDINRFPDSDKLARFMGLAPVQFSSAGKGKDQRCRNGNRALNAIFHFLAIQMVAVSASGKPRHPVFREYFEQKVKEGKNKPQALVCVARRLVRIIYG
MMKTKTEYRPYEKTDDKN
AYTSAVRLANPIIYKDDSYDAYCVARVLRDMVDTLQDAKHEDIFWTIRQMVKRRDLIVKSNVMNKNQLHSQLAYSYPSYRKFFAMIDSKSALCFWENYPS
PEYIWKTTPEEIYQTIKPVHQALKIQRIHEIISMIERDGDTRKDYQPERDFIVRNIVKDIRHNKELIAEIDDELRKLIPLTGYKLHTMPGIDLVTEAQII
SEIGDINRFPDSDKLARFMGLAPVQFSSAGKGKDQRCRNGNRALNAIFHFLAIQMVAVSASGKPRHPVFREYFEQKVKEGKNKPQALVCVARRLVRIIYG
MMKTKTEYRPYEKTDDKN
Blast result :
Comments
ISCth6 is 48% aa similar to IS653.There are 3 copies in the genome.
References
1] Miriam Land (2009) Direct submission
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.