ISPmi1

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
EU917444 ND Proteus mirabilis
Proteus mirabilis
DNA section
IS Length : 1306 bp

Ends


IR Length : 23/26

IRL : TGAATCGCCCCGGGTTTGTCGGAGGCTAACTTTCTTGAGAGAATTAGCCT
IRR : TGAATCGCCCCGACTTTTTCGGAGGCGGTTTAATTTGAGTCATGCTACAT

Insertion site


Left flankDirect repeatRight flankDR Length
TTTGTTGTCAATATGGCTGTAGA3

DNA sequence

TGAATCGCCCCGGGTTTGTCGGAGGCTAACTTTCTTGAGAGAATTAGCCTATGAAAAAGAGACCTGGATATTCCCCCGAAACGCGTGAACGAGCAGTAAG
GCTTGTGCTCACCACCGAACAAGATCACAGCTCACGTTGGGCGGCCATAATATCTGTTGCCAGCAAGATTGGCTGTACGCCTGAAACACTCCGCGCATGG
ATAAACCGAACTGAATCCAATAGCGCCGCTCCCGACACCGTAAACGTTACCGAATCCGAGCGAATTAAAGCCTTAGAGCGCGAGAATCGCGAACTCAAAC
GTGCGAATGAAATCCTGCGTCTAGCCTCGGCTTTTTTTGCCCAGGCGGAGCTCGACCGCAAACCGAAACTCTAGTCCATTTTGTGGAAGCCCATAAAAAT
GATTTTGGTGTCGAGCCAATTTGTCAGCAGTTACAGATTGCACCATCGACGTATTATCGACACAAGCAATTGGAGAAAAAGCCGGAGCTGAGAGCGCGGC
GAATTCAGCAGGATGAGTGTTTAGCCGTTGAAATCCAGCGTATTTGGCTTGAAAGTGATCGTAATTATGGTGCACGGAAAATCTGGAAACAGCTGCGCAG
AGAGGGGGTTGATGTGGCACGGTGTACCGTTGAGCGGCTAATGCGTCAATTGGGTGTTGAAGGTGTGCGTCGCGGCAAAAAATGTAAAACAACAATCCCT
GACAACAAAGCACATTGCCCGCAAGATCTGGTTAACCGTCACTTTAAAGCGGAGAAGCCAAATCAACTCTGGGTTGCCGACATTACTTATGTTTCCACCT
GGTCAGGTTTTGTCTATGTCGCCTTTGTAATCGATGTATTTTCGCGTCGCCTTGTTGGCTGGCGAGCGATGAAGACTATGCAGACGGATTTAATTCTGGA
CGCACTGGAGCAGGCATTGTGGGCAAGAGGAAAACCGCGCGGCGTGATTCATCACAGTGACAGGGGTAGTCAATATTTATCCATCAGCTACACCGAACGC
CTTGCTGAGGCAGGATTTAATGCGTCGGTTGGGAGCGTGGGGGATTCCTACGATAACGCCTTAGCTGAAACGATTAACGGGCTTTACAAAACTGAGGTTA
TTCATAAAGGCGCACCTTGGAAAAATCTTGAATCTGTAGAGCTGGCGACGCTTGCATGGGTCGAATGGTTTAACAACCGTCGGCTGCATAGTGCACTGGG
GTATGTACCACCGAAGGAGTTCGAAGAAAATTTTTATCAACAAACTGAGTTGGCTCATGTAGCATGACTCAAATTAAACCGCCTCCGAAAAAGTCGGGGC
GATTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
324 bp107 aa51374+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKRPGYSPETRERAVRLVLTTEQDHSSRWAAIISVASKIGCTPETLRAWINRTESNSAAPDTVNVTESERIKALERENRELKRANEILRLASAFFAQAE
LDRKPKL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
957 bp318 aa3111267+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

NPASSLGFFCPGGARPQTETLVHFVEAHKNDFGVEPICQQLQIAPSTYYRHKQLEKKPELRARRIQQDECLAVEIQRIWLESDRNYGARKIWKQLRREGV
DVARCTVERLMRQLGVEGVRRGKKCKTTIPDNKAHCPQDLVNRHFKAEKPNQLWVADITYVSTWSGFVYVAFVIDVFSRRLVGWRAMKTMQTDLILDALE
QALWARGKPRGVIHHSDRGSQYLSISYTERLAEAGFNASVGSVGDSYDNALAETINGLYKTEVIHKGAPWKNLESVELATLAWVEWFNNRRLHSALGYVP
PKEFEENFYQQTELAHVA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1217 bp405 aa511267+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKRPGYSPETRERAVRLVLTTEQDHSSRWAAIISVASKIGCTPETLRAWINRTESNSAAPDTVNVTESERIKALERENRELKRANEILRLASAFFCPGG
ARPQTETLVHFVEAHKNDFGVEPICQQLQIAPSTYYRHKQLEKKPELRARRIQQDECLAVEIQRIWLESDRNYGARKIWKQLRREGVDVARCTVERLMRQ
LGVEGVRRGKKCKTTIPDNKAHCPQDLVNRHFKAEKPNQLWVADITYVSTWSGFVYVAFVIDVFSRRLVGWRAMKTMQTDLILDALEQALWARGKPRGVI
HHSDRGSQYLSISYTERLAEAGFNASVGSVGDSYDNALAETINGLYKTEVIHKGAPWKNLESVELATLAWVEWFNNRRLHSALGYVPPKEFEENFYQQTE
LAHVA

 

Blast result :
Comments
ISPmi1 ORF1 is 59% aa similar to ISPsy21 (ORF1) and ORF2 56% to ISPsy21(ORF2). The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Wang M.H., Guo Q.L.,Wang M.G. (2008) Direct submission.
2] Mazauric, M. H., P. Licznar, M. F. Prere, I. Canal, and O. Fayet. (2008). J. Biol. Chem., 283: 20421-20432.
3] Chen, C. C., and S. T. Hu. (2006). J. Biol. Chem.281:21617-C21628.