ISPmi1
- Family IS3
- Group IS51
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
EU917444 | ND | Proteus mirabilis | Proteus mirabilis |
DNA section
IS Length : 1306 bp
Ends
IR Length : 23/26
IRL : TGAATCGCCCCGGGTTTGTCGGAGGCTAACTTTCTTGAGAGAATTAGCCT
IRR : TGAATCGCCCCGACTTTTTCGGAGGCGGTTTAATTTGAGTCATGCTACAT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TTTGTTGTCA | ATA | TGGCTGTAGA | 3 |
DNA sequence
TGAATCGCCCCGGGTTTGTCGGAGGCTAACTTTCTTGAGAGAATTAGCCTATGAAAAAGAGACCTGGATATTCCCCCGAAACGCGTGAACGAGCAGTAAG
GCTTGTGCTCACCACCGAACAAGATCACAGCTCACGTTGGGCGGCCATAATATCTGTTGCCAGCAAGATTGGCTGTACGCCTGAAACACTCCGCGCATGG
ATAAACCGAACTGAATCCAATAGCGCCGCTCCCGACACCGTAAACGTTACCGAATCCGAGCGAATTAAAGCCTTAGAGCGCGAGAATCGCGAACTCAAAC
GTGCGAATGAAATCCTGCGTCTAGCCTCGGCTTTTTTTGCCCAGGCGGAGCTCGACCGCAAACCGAAACTCTAGTCCATTTTGTGGAAGCCCATAAAAAT
GATTTTGGTGTCGAGCCAATTTGTCAGCAGTTACAGATTGCACCATCGACGTATTATCGACACAAGCAATTGGAGAAAAAGCCGGAGCTGAGAGCGCGGC
GAATTCAGCAGGATGAGTGTTTAGCCGTTGAAATCCAGCGTATTTGGCTTGAAAGTGATCGTAATTATGGTGCACGGAAAATCTGGAAACAGCTGCGCAG
AGAGGGGGTTGATGTGGCACGGTGTACCGTTGAGCGGCTAATGCGTCAATTGGGTGTTGAAGGTGTGCGTCGCGGCAAAAAATGTAAAACAACAATCCCT
GACAACAAAGCACATTGCCCGCAAGATCTGGTTAACCGTCACTTTAAAGCGGAGAAGCCAAATCAACTCTGGGTTGCCGACATTACTTATGTTTCCACCT
GGTCAGGTTTTGTCTATGTCGCCTTTGTAATCGATGTATTTTCGCGTCGCCTTGTTGGCTGGCGAGCGATGAAGACTATGCAGACGGATTTAATTCTGGA
CGCACTGGAGCAGGCATTGTGGGCAAGAGGAAAACCGCGCGGCGTGATTCATCACAGTGACAGGGGTAGTCAATATTTATCCATCAGCTACACCGAACGC
CTTGCTGAGGCAGGATTTAATGCGTCGGTTGGGAGCGTGGGGGATTCCTACGATAACGCCTTAGCTGAAACGATTAACGGGCTTTACAAAACTGAGGTTA
TTCATAAAGGCGCACCTTGGAAAAATCTTGAATCTGTAGAGCTGGCGACGCTTGCATGGGTCGAATGGTTTAACAACCGTCGGCTGCATAGTGCACTGGG
GTATGTACCACCGAAGGAGTTCGAAGAAAATTTTTATCAACAAACTGAGTTGGCTCATGTAGCATGACTCAAATTAAACCGCCTCCGAAAAAGTCGGGGC
GATTCA
GCTTGTGCTCACCACCGAACAAGATCACAGCTCACGTTGGGCGGCCATAATATCTGTTGCCAGCAAGATTGGCTGTACGCCTGAAACACTCCGCGCATGG
ATAAACCGAACTGAATCCAATAGCGCCGCTCCCGACACCGTAAACGTTACCGAATCCGAGCGAATTAAAGCCTTAGAGCGCGAGAATCGCGAACTCAAAC
GTGCGAATGAAATCCTGCGTCTAGCCTCGGCTTTTTTTGCCCAGGCGGAGCTCGACCGCAAACCGAAACTCTAGTCCATTTTGTGGAAGCCCATAAAAAT
GATTTTGGTGTCGAGCCAATTTGTCAGCAGTTACAGATTGCACCATCGACGTATTATCGACACAAGCAATTGGAGAAAAAGCCGGAGCTGAGAGCGCGGC
GAATTCAGCAGGATGAGTGTTTAGCCGTTGAAATCCAGCGTATTTGGCTTGAAAGTGATCGTAATTATGGTGCACGGAAAATCTGGAAACAGCTGCGCAG
AGAGGGGGTTGATGTGGCACGGTGTACCGTTGAGCGGCTAATGCGTCAATTGGGTGTTGAAGGTGTGCGTCGCGGCAAAAAATGTAAAACAACAATCCCT
GACAACAAAGCACATTGCCCGCAAGATCTGGTTAACCGTCACTTTAAAGCGGAGAAGCCAAATCAACTCTGGGTTGCCGACATTACTTATGTTTCCACCT
GGTCAGGTTTTGTCTATGTCGCCTTTGTAATCGATGTATTTTCGCGTCGCCTTGTTGGCTGGCGAGCGATGAAGACTATGCAGACGGATTTAATTCTGGA
CGCACTGGAGCAGGCATTGTGGGCAAGAGGAAAACCGCGCGGCGTGATTCATCACAGTGACAGGGGTAGTCAATATTTATCCATCAGCTACACCGAACGC
CTTGCTGAGGCAGGATTTAATGCGTCGGTTGGGAGCGTGGGGGATTCCTACGATAACGCCTTAGCTGAAACGATTAACGGGCTTTACAAAACTGAGGTTA
TTCATAAAGGCGCACCTTGGAAAAATCTTGAATCTGTAGAGCTGGCGACGCTTGCATGGGTCGAATGGTTTAACAACCGTCGGCTGCATAGTGCACTGGG
GTATGTACCACCGAAGGAGTTCGAAGAAAATTTTTATCAACAAACTGAGTTGGCTCATGTAGCATGACTCAAATTAAACCGCCTCCGAAAAAGTCGGGGC
GATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
324 bp | 107 aa | 51 | 374 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRPGYSPETRERAVRLVLTTEQDHSSRWAAIISVASKIGCTPETLRAWINRTESNSAAPDTVNVTESERIKALERENRELKRANEILRLASAFFAQAE
LDRKPKL
LDRKPKL
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
957 bp | 318 aa | 311 | 1267 | + | No |
Description : Second part of the transposase
ORF sequence :
NPASSLGFFCPGGARPQTETLVHFVEAHKNDFGVEPICQQLQIAPSTYYRHKQLEKKPELRARRIQQDECLAVEIQRIWLESDRNYGARKIWKQLRREGV
DVARCTVERLMRQLGVEGVRRGKKCKTTIPDNKAHCPQDLVNRHFKAEKPNQLWVADITYVSTWSGFVYVAFVIDVFSRRLVGWRAMKTMQTDLILDALE
QALWARGKPRGVIHHSDRGSQYLSISYTERLAEAGFNASVGSVGDSYDNALAETINGLYKTEVIHKGAPWKNLESVELATLAWVEWFNNRRLHSALGYVP
PKEFEENFYQQTELAHVA
DVARCTVERLMRQLGVEGVRRGKKCKTTIPDNKAHCPQDLVNRHFKAEKPNQLWVADITYVSTWSGFVYVAFVIDVFSRRLVGWRAMKTMQTDLILDALE
QALWARGKPRGVIHHSDRGSQYLSISYTERLAEAGFNASVGSVGDSYDNALAETINGLYKTEVIHKGAPWKNLESVELATLAWVEWFNNRRLHSALGYVP
PKEFEENFYQQTELAHVA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1217 bp | 405 aa | 51 | 1267 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRPGYSPETRERAVRLVLTTEQDHSSRWAAIISVASKIGCTPETLRAWINRTESNSAAPDTVNVTESERIKALERENRELKRANEILRLASAFFCPGG
ARPQTETLVHFVEAHKNDFGVEPICQQLQIAPSTYYRHKQLEKKPELRARRIQQDECLAVEIQRIWLESDRNYGARKIWKQLRREGVDVARCTVERLMRQ
LGVEGVRRGKKCKTTIPDNKAHCPQDLVNRHFKAEKPNQLWVADITYVSTWSGFVYVAFVIDVFSRRLVGWRAMKTMQTDLILDALEQALWARGKPRGVI
HHSDRGSQYLSISYTERLAEAGFNASVGSVGDSYDNALAETINGLYKTEVIHKGAPWKNLESVELATLAWVEWFNNRRLHSALGYVPPKEFEENFYQQTE
LAHVA
ARPQTETLVHFVEAHKNDFGVEPICQQLQIAPSTYYRHKQLEKKPELRARRIQQDECLAVEIQRIWLESDRNYGARKIWKQLRREGVDVARCTVERLMRQ
LGVEGVRRGKKCKTTIPDNKAHCPQDLVNRHFKAEKPNQLWVADITYVSTWSGFVYVAFVIDVFSRRLVGWRAMKTMQTDLILDALEQALWARGKPRGVI
HHSDRGSQYLSISYTERLAEAGFNASVGSVGDSYDNALAETINGLYKTEVIHKGAPWKNLESVELATLAWVEWFNNRRLHSALGYVPPKEFEENFYQQTE
LAHVA
Blast result :
Comments
ISPmi1 ORF1 is 59% aa similar to ISPsy21 (ORF1) and ORF2 56% to ISPsy21(ORF2). The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Wang M.H., Guo Q.L.,Wang M.G. (2008) Direct submission.
2] Mazauric, M. H., P. Licznar, M. F. Prere, I. Canal, and O. Fayet. (2008). J. Biol. Chem., 283: 20421-20432.
3] Chen, C. C., and S. T. Hu. (2006). J. Biol. Chem.281:21617-C21628.
2] Mazauric, M. H., P. Licznar, M. F. Prere, I. Canal, and O. Fayet. (2008). J. Biol. Chem., 283: 20421-20432.
3] Chen, C. C., and S. T. Hu. (2006). J. Biol. Chem.281:21617-C21628.