ISMex7
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium extorquens | Methylobacterium extorquens AM1 |
DNA section
IS Length : 1285 bp
Ends
IR Length : 22/27
IRL : GAGAGCTGGCTCGGGAAAACTGGACAGGCCGGATAAGTGGAGTTTCTGCC
IRR : GAGAGCTGGCTCGGTTGATCTGAACAGTTTTGGCGCTGTCGCTAAGTGGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCCCATGGC | TACTTCGGCA | 0 |
DNA sequence
GAGAGCTGGCTCGGGAAAACTGGACAGGCCGGATAAGTGGAGTTTCTGCCTGAAGGCGGCATTATCGCCGCGACAGGAGAGACCATGGCGAAACGACCGC
GGCGGAACCACACGCCGGCCTTCAAGGCGAAGGTGGCCCTTGCAGCCGTGAAAGGCGAGAAGACGATCGTCGAGCTGGCACAGCAGTTCGACGTGCATCC
GAACCAGATCACGCAATGGCGCAATCAGCTTCTCGACGGGGCTGCGGGCGTGTTCGACGGCGAGGCGAAGGCCGCGGCGCCGGCTCCGATGATCGACGTG
AAGACGCTGCACGCGAAGATCGGGGAACTGACGCTGGAGAATGATTTTTTGTCCGGCGCGCTCGGGAAGGCCGGCCTTCTGAGCGCAAAGCGATGATCGA
CCGTCATCACGACCTGCCGCTCAGCCGGCAGGCGAAGGTGCTGGGGATCAGCCGGGGCAGCGTCTACTATCTGCCCCGCGCGGTTCCGGCTGCCGATCTG
GCTCTGATGCGCCGGATCGATGCGCTGCATCTGGAATATCCCTTCGCCGGCAGCCGGATGTTGCAAGGGCTTCTCAATGCCGAGGGTCATGTCGCCGGGC
GCCTGCACGTCGCCACGCTGATGAAGCGCATGGGGATCGAGGCGCTCTATCGCAGACCGAGGACCTCGAAGCGGGCGCCTGGGCACGCGATCTTCCCCTA
CCTGCTGCGCAAGCTGCCGGTGACGCGTCCCAATCAGGTCTGGGCGATGGACATCACCTACATCCCGATGGCGCGGGGCTTCGTCTATCTCGCCGCCGTC
GTCGATTGGTTCAGCCGCAAGGTGCTGGCCTGGCGGCTCTCGATCACGATGGACACCACCTTCTGCATCGAGGCGGTCGAGGACGCCATGGCCCGCTTTG
GCAAGCCGGAGATCTTCAATACGGACCAGGGCTCGCAGTTCACCAGCCGCGAGTTCACCGGCCTGCTGATCGCAGCCGGCATCAGGATCAGCATGGACGG
CAAGGGTGCCTGGCGCGACAACGTCTTCGTCGAACGCCTCTGGAAATCAGTCAAATACGAGGAGGTCTATCTGCGCGCCTATGCCAGCGTGTCGGAGGCC
CGTGCCTCGATCGGCCGGTATCTCGACTTCTTCAACACCCGGAGGCCTCATCAGGGCTTGGGACGCCAGACGCCGGACCAAGCCTACTTCAACGCGCTGC
TGCCAATCCCGGCGGTGGCATAACCAGGGCTGAAACCCACTTAGCGACAGCGCCAAAACTGTTCAGATCAACCGAGCCAGCTCTC
GGCGGAACCACACGCCGGCCTTCAAGGCGAAGGTGGCCCTTGCAGCCGTGAAAGGCGAGAAGACGATCGTCGAGCTGGCACAGCAGTTCGACGTGCATCC
GAACCAGATCACGCAATGGCGCAATCAGCTTCTCGACGGGGCTGCGGGCGTGTTCGACGGCGAGGCGAAGGCCGCGGCGCCGGCTCCGATGATCGACGTG
AAGACGCTGCACGCGAAGATCGGGGAACTGACGCTGGAGAATGATTTTTTGTCCGGCGCGCTCGGGAAGGCCGGCCTTCTGAGCGCAAAGCGATGATCGA
CCGTCATCACGACCTGCCGCTCAGCCGGCAGGCGAAGGTGCTGGGGATCAGCCGGGGCAGCGTCTACTATCTGCCCCGCGCGGTTCCGGCTGCCGATCTG
GCTCTGATGCGCCGGATCGATGCGCTGCATCTGGAATATCCCTTCGCCGGCAGCCGGATGTTGCAAGGGCTTCTCAATGCCGAGGGTCATGTCGCCGGGC
GCCTGCACGTCGCCACGCTGATGAAGCGCATGGGGATCGAGGCGCTCTATCGCAGACCGAGGACCTCGAAGCGGGCGCCTGGGCACGCGATCTTCCCCTA
CCTGCTGCGCAAGCTGCCGGTGACGCGTCCCAATCAGGTCTGGGCGATGGACATCACCTACATCCCGATGGCGCGGGGCTTCGTCTATCTCGCCGCCGTC
GTCGATTGGTTCAGCCGCAAGGTGCTGGCCTGGCGGCTCTCGATCACGATGGACACCACCTTCTGCATCGAGGCGGTCGAGGACGCCATGGCCCGCTTTG
GCAAGCCGGAGATCTTCAATACGGACCAGGGCTCGCAGTTCACCAGCCGCGAGTTCACCGGCCTGCTGATCGCAGCCGGCATCAGGATCAGCATGGACGG
CAAGGGTGCCTGGCGCGACAACGTCTTCGTCGAACGCCTCTGGAAATCAGTCAAATACGAGGAGGTCTATCTGCGCGCCTATGCCAGCGTGTCGGAGGCC
CGTGCCTCGATCGGCCGGTATCTCGACTTCTTCAACACCCGGAGGCCTCATCAGGGCTTGGGACGCCAGACGCCGGACCAAGCCTACTTCAACGCGCTGC
TGCCAATCCCGGCGGTGGCATAACCAGGGCTGAAACCCACTTAGCGACAGCGCCAAAACTGTTCAGATCAACCGAGCCAGCTCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 312 bp | 103 aa | 85 | 396 | + | No |
Description : First part of the transposase
ORF sequence :
MAKRPRRNHTPAFKAKVALAAVKGEKTIVELAQQFDVHPNQITQWRNQLLDGAAGVFDGEAKAAAPAPMIDVKTLHAKIGELTLENDFLSGALGKAGLLS
AKR
AKR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 879 bp | 292 aa | 345 | 1223 | + | No |
Description : Second part of the transposase
ORF sequence :
FFVRRAREGRPSERKAMIDRHHDLPLSRQAKVLGISRGSVYYLPRAVPAADLALMRRIDALHLEYPFAGSRMLQGLLNAEGHVAGRLHVATLMKRMGIEA
LYRRPRTSKRAPGHAIFPYLLRKLPVTRPNQVWAMDITYIPMARGFVYLAAVVDWFSRKVLAWRLSITMDTTFCIEAVEDAMARFGKPEIFNTDQGSQFT
SREFTGLLIAAGIRISMDGKGAWRDNVFVERLWKSVKYEEVYLRAYASVSEARASIGRYLDFFNTRRPHQGLGRQTPDQAYFNALLPIPAVA
LYRRPRTSKRAPGHAIFPYLLRKLPVTRPNQVWAMDITYIPMARGFVYLAAVVDWFSRKVLAWRLSITMDTTFCIEAVEDAMARFGKPEIFNTDQGSQFT
SREFTGLLIAAGIRISMDGKGAWRDNVFVERLWKSVKYEEVYLRAYASVSEARASIGRYLDFFNTRRPHQGLGRQTPDQAYFNALLPIPAVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1139 bp | 379 aa | 85 | 1223 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKRPRRNHTPAFKAKVALAAVKGEKTIVELAQQFDVHPNQITQWRNQLLDGAAGVFDGEAKAAAPAPMIDVKTLHAKIGELTLENDFFVRRAREGRPSE
RKAMIDRHHDLPLSRQAKVLGISRGSVYYLPRAVPAADLALMRRIDALHLEYPFAGSRMLQGLLNAEGHVAGRLHVATLMKRMGIEALYRRPRTSKRAPG
HAIFPYLLRKLPVTRPNQVWAMDITYIPMARGFVYLAAVVDWFSRKVLAWRLSITMDTTFCIEAVEDAMARFGKPEIFNTDQGSQFTSREFTGLLIAAGI
RISMDGKGAWRDNVFVERLWKSVKYEEVYLRAYASVSEARASIGRYLDFFNTRRPHQGLGRQTPDQAYFNALLPIPAVA
RKAMIDRHHDLPLSRQAKVLGISRGSVYYLPRAVPAADLALMRRIDALHLEYPFAGSRMLQGLLNAEGHVAGRLHVATLMKRMGIEALYRRPRTSKRAPG
HAIFPYLLRKLPVTRPNQVWAMDITYIPMARGFVYLAAVVDWFSRKVLAWRLSITMDTTFCIEAVEDAMARFGKPEIFNTDQGSQFTSREFTGLLIAAGI
RISMDGKGAWRDNVFVERLWKSVKYEEVYLRAYASVSEARASIGRYLDFFNTRRPHQGLGRQTPDQAYFNALLPIPAVA
Blast result :
Comments
ISMex7 is 83% ISKpn11.
Ends are not typical of the family.The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Ends are not typical of the family.The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References