ISMex2

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
DNA section
IS Length : 1031 bp

Ends


IR Length : 16/23

IRL : TGTGATCTTTCCATAGGTTGTCCATCCGGTCCGGACCGAGGAAGCTGAGG
IRR : TGTGGATGCTCAACAGGTTGTCCTCTGTCAGGCGGATGCGGCTGATGGGT

Insertion site


Left flankDirect repeatRight flankDR Length
GGAAGGCGCt/gCTAGCGCCACCCCT6
GAGCGCGCCt/gCTAGCCTGTGGATCA6
ACATCGCTGc/gCTAGc/aGCCGTCTCC8
TATTCGGCCa/gCTAGc/aGCCGCCACC8
GCGCCAAACGCTAGc/aGCGCATTCC6
GCGGGGCGCt/gCTAGc/aTCCGGCGAA8
CCCTTCGCGGCTAGc/aGCCTCACGG6
GTGCGCCGCt/gCTAGCGCACCCGTG6
CCCAATTGCt/gCTAGCGTCGGGGTG6
CGCTGCTGAt/gCTAGc/gTCCTGCGCG8
GGAATGCGCt/gCTAGc/aACGACGGCT8
GCAACAAAAc/gCTAGc/aGCGGCACAG8
GGATGATGCt/gCTAGCGGCCGGTGC6
GGACGACGCt/gCTAGCGGTACGGGG6
CGCTGCTGCGCTAGc/aGCGCTTTCC6
CGGTGCGGCt/gCTAGc/aGGAAAGACC8

DNA sequence

TGTGATCTTTCCATAGGTTGTCCATCCGGTCCGGACCGAGGAAGCTGAGGTTGCGAAGCTTCAGACCCCTTGGAGGGACCGGATGAACATCCACCAGAAT
GCGCGTTTGACGCCGTCCGGTCGAGAGAGGCTGGTGCGGTTGGCCCGGAGTGGCCTCGCCCCGCAGGCGGTGGCTGAGAGGATGGGGGTCTGTACCAAGA
CGGTCCGCAAGTGGGTCGCCCGCTTTGAAGCCGAAGGCACCGCCGGGCTGCAGGATCGCTCCTCGCGGCCCCGCGGCCTGTACCGGCCGACCCCGGCCGA
GACACAGGCGGCCATCGTAGCCCTGCGTCGGCAGCGCCTGACCGGCGCGCAGATCGCGCGCGACTTGGCCGTCTCGCCCGCCACCGTCAGCCGGGTCCTG
CGTCGGCACGGCCTGAGCCGCATCCGCGATCTCGAACCGCCGCCACCCGTTCAGCGCTACGAGCGTGAGCGGCCAGGCGAGTTGATCCACATCGACATCA
AGAAGCTCGGTCGCTTCGCACGAACCGGTCATCGCATCACCGGCGACCGCACCCGGCAGAGTTCAGGCCGCGGCATCGGCTGGGAGTTCCTGCACCTGGC
CATCGACGACCATTCTCGGCTGGCCTACTCCGAGATCCTGCCCGACGAGACCCGGGCCTCCTGCCTCGCCTTCCTCTTCAACGCCCTGCGGTTCTTCCGC
CGCCACGGTATCGGTGTCGAGCGCGTCATGACCGACAACGGGCCAGCCTTCAAATCCAAGCGCTACGCCAAGGCCTTGCGGCGCCTCCACATCGGCCACA
AGCGCACGCGACCCTACACGCCGAGGACCAACGGCAAGGTCGAGCGCTTCGTCCAGACCTCCCTGCGCGAATGGGCGTACGCCCGGGCCTACGACACCTC
CGAGCAGCGCCGCGCCGAACTGCCCGCCTTCTTGTTCCGCTACAACTGGCAGCGCCCACACGGCGGAATCAACGGCAGCACACCCATCAGCCGCATCCGC
CTGACAGAGGACAACCTGTTGAGCATCCACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
902 bp300 aa1341035+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VRLARSGLAPQAVAERMGVCTKTVRKWVARFEAEGTAGLQDRSSRPRGLYRPTPAETQAAIVALRRQRLTGAQIARDLAVSPATVSRVLRRHGLSRIRDL
EPPPPVQRYERERPGELIHIDIKKLGRFARTGHRITGDRTRQSSGRGIGWEFLHLAIDDHSRLAYSEILPDETRASCLAFLFNALRFFRRHGIGVERVMT
DNGPAFKSKRYAKALRRLHIGHKRTRPYTPRTNGKVERFVQTSLREWAYARAYDTSEQRRAELPAFLFRYNWQRPHGGINGSTPISRIRLTEDNLLSIHS

 

Blast result :
Comments
The end of the transposase is in the target site : CTAG. This IS has 16 copies in AM1 and all of them end with Tpase ORF.
ISMex2 is 71% aa similar to ISRel2.
References