ISVsa21
- Family IS91
- Group
Isoform Synonym(s) Vsa3
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011311 | ND | Aliivibrio salmonicida | Aliivibrio salmonicida LFI1238 plasmid pVSAL840 |
DNA section
IS Length : 1353 bp
Ends
oriIS : ACAATAACGTGACAACATCACGTTAGCCTTTTCTATGTACGACCCTAATCATTTTAAACATCTATTTACTCTTGGCAGGTGCTTCTATAAAGCATACAAA II struct. : No
terIS : TTTTGAGTAAAAAACAAACAGGTTGGGTGTATTTAGAGAATTAGAAATAATTGGTCTTGTCGAATGTCCTTCGAGGACTGAAGGTATGGCTCGATATTGT II struct. : No
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| ACGCTGCAACCCTTTCGAAT | TGTTGCTCTCAACTTTG |
DNA sequence
ACAATAACGTGACAACATCACGTTAGCCTTTTCTATGTACGACCCTAATCATTTTAAACATCTATTTACTCTTGGCAGGTGCTTCTATAAAGCATACAAA
GATAATTACCCTTTTCGCACCGTAGAATTAGAAGCCGTCGTAAAATATCTTTCTTGCAAAACAGATCGCTTAGGGAAGCTAACCTATTCATGCAGAAACA
ACGCCTGCTCACACGTAAAATACGTTTTTAGTACTTGTAAGAGTAAGTTATGCCCCTCGTGTGGCCAAAAGGCCACAGAGCGTTGGATAGCAGCTCAGAG
TGAAATACTTCCAGATTGTAAATTCCGTCATATTACCTTCACTATGCCTGGTCAGTTTTGGCCCTTATTTCAATTAAACCGATGGCTCTTGGGGGATCTT
TTTTCTATTTCTGCAAATACGTTACTCACTCAGGCGAAAACTAAAAAATTAACCATAGGTATTTTTGCGGCTCTTCATACCTATGGTCGGAAGCTAAACT
TTAATTGTCATATTCACTTAGCATTAGCTGAATTAGGACTGGATAAGCATGAAAACTTAAAAAAGTTTTCATTTAAGTTTGCTTCATTAATGAAGCAATG
GCGATACGGGATAATCAAACTACTGAGAGATAATTATGACCGACTGATATTCCCATCAGAGCTTAGCGATGAAGCAAAAAACACTCAGTCTTGGAATGGT
TTTTTAAACACCCAATATAATCGTCATTGGAATGTGAATATCGCCAAAAAGACAAGCCATAAGAAACATACCGCTAAATATTTAGGAAGTTACGTAAAGA
AGCCGCCGATAGCGGCCTCTCGCCTAAAAGAATATGCGAATGGGGAGGTCACGTTCACCTACTTAGACCACAACAGCAAAAAACATAAAGAGTTATCGCT
TACTCAAACAGAAATGATGTTGAGGGTGCTGAGCCATGTCCCAGAAAAATATTTTAAGATGATACGTTACTTCGGTTTTTTATCGACACGTTTACGTGGC
GATATGCTTCCAATTATTTATGAAAAGCTAGAGCAAAAAGTGAATAAACCAATGGTTTTTAGTTTTGCATCGATGATGAAAGCTCTTATGAAAGTAGACC
CATTTGAATGTATTTTATGTGGTTCAAAAATGTTTTTCAGCGGATTTATCCGAGGGCCTAAGCTTGGTCAATTGGTCAGTTCAATAGAAAACCTAGCGTT
ATTAAGGCCAATGTAGACAGCATGACTAGGATAGAACCGTCTTTTTGATGTCATTTTGAGTAAAAAACAAACAGGTTGGGTGTATTTAGAGAATTAGAAA
TAATTGGTCTTGTCGAATGTCCTTCGAGGACTGAAGGTATGGCTCGATATTGT
GATAATTACCCTTTTCGCACCGTAGAATTAGAAGCCGTCGTAAAATATCTTTCTTGCAAAACAGATCGCTTAGGGAAGCTAACCTATTCATGCAGAAACA
ACGCCTGCTCACACGTAAAATACGTTTTTAGTACTTGTAAGAGTAAGTTATGCCCCTCGTGTGGCCAAAAGGCCACAGAGCGTTGGATAGCAGCTCAGAG
TGAAATACTTCCAGATTGTAAATTCCGTCATATTACCTTCACTATGCCTGGTCAGTTTTGGCCCTTATTTCAATTAAACCGATGGCTCTTGGGGGATCTT
TTTTCTATTTCTGCAAATACGTTACTCACTCAGGCGAAAACTAAAAAATTAACCATAGGTATTTTTGCGGCTCTTCATACCTATGGTCGGAAGCTAAACT
TTAATTGTCATATTCACTTAGCATTAGCTGAATTAGGACTGGATAAGCATGAAAACTTAAAAAAGTTTTCATTTAAGTTTGCTTCATTAATGAAGCAATG
GCGATACGGGATAATCAAACTACTGAGAGATAATTATGACCGACTGATATTCCCATCAGAGCTTAGCGATGAAGCAAAAAACACTCAGTCTTGGAATGGT
TTTTTAAACACCCAATATAATCGTCATTGGAATGTGAATATCGCCAAAAAGACAAGCCATAAGAAACATACCGCTAAATATTTAGGAAGTTACGTAAAGA
AGCCGCCGATAGCGGCCTCTCGCCTAAAAGAATATGCGAATGGGGAGGTCACGTTCACCTACTTAGACCACAACAGCAAAAAACATAAAGAGTTATCGCT
TACTCAAACAGAAATGATGTTGAGGGTGCTGAGCCATGTCCCAGAAAAATATTTTAAGATGATACGTTACTTCGGTTTTTTATCGACACGTTTACGTGGC
GATATGCTTCCAATTATTTATGAAAAGCTAGAGCAAAAAGTGAATAAACCAATGGTTTTTAGTTTTGCATCGATGATGAAAGCTCTTATGAAAGTAGACC
CATTTGAATGTATTTTATGTGGTTCAAAAATGTTTTTCAGCGGATTTATCCGAGGGCCTAAGCTTGGTCAATTGGTCAGTTCAATAGAAAACCTAGCGTT
ATTAAGGCCAATGTAGACAGCATGACTAGGATAGAACCGTCTTTTTGATGTCATTTTGAGTAAAAAACAAACAGGTTGGGTGTATTTAGAGAATTAGAAA
TAATTGGTCTTGTCGAATGTCCTTCGAGGACTGAAGGTATGGCTCGATATTGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1192 bp | 393 aa | 25 | 1216 | + | No |
Chemistry : Y2
ORF sequence :
MYDPNHFKHLFTLGRCFYKAYKDNYPFRTVELEAVVKYLSCKTDRLGKLTYSCRNNACSHVKYVFSTCKSKLCPSCGQKATERWIAAQSEILPDCKFRHI
TFTMPGQFWPLFQLNRWLLGDLFSISANTLLTQAKTKKLTIGIFAALHTYGRKLNFNCHIHLALAELGLDKHENLKKFSFKFASLMKQWRYGIIKLLRDN
YDRLIFPSELSDEAKNTQSWNGFLNTQYNRHWNVNIAKKTSHKKHTAKYLGSYVKKPPIAASRLKEYANGEVTFTYLDHNSKKHKELSLTQTEMMLRVLS
HVPEKYFKMIRYFGFLSTRLRGDMLPIIYEKLEQKVNKPMVFSFASMMKALMKVDPFECILCGSKMFFSGFIRGPKLGQLVSSIENLALLRPM
TFTMPGQFWPLFQLNRWLLGDLFSISANTLLTQAKTKKLTIGIFAALHTYGRKLNFNCHIHLALAELGLDKHENLKKFSFKFASLMKQWRYGIIKLLRDN
YDRLIFPSELSDEAKNTQSWNGFLNTQYNRHWNVNIAKKTSHKKHTAKYLGSYVKKPPIAASRLKEYANGEVTFTYLDHNSKKHKELSLTQTEMMLRVLS
HVPEKYFKMIRYFGFLSTRLRGDMLPIIYEKLEQKVNKPMVFSFASMMKALMKVDPFECILCGSKMFFSGFIRGPKLGQLVSSIENLALLRPM
Blast result :
Comments
ISVsa21 is 62% aa similar to ISVsa9.
References
1] ISfinder annotation (2009)
2] Hjerde,E., Lorentzen,M.S., Holden,M.T.G., Seeger,K., Paulsen,S., Bason,N., Churcher,C., Harris,D., Norbertczak,H., Quail,M.A., Sanders,S., Thurston,S., Parkhill,J., Willassen,N.P. and Thomson,N.R. (2008) Direct submission GenBank.
2] Hjerde,E., Lorentzen,M.S., Holden,M.T.G., Seeger,K., Paulsen,S., Bason,N., Churcher,C., Harris,D., Norbertczak,H., Quail,M.A., Sanders,S., Thurston,S., Parkhill,J., Willassen,N.P. and Thomson,N.R. (2008) Direct submission GenBank.