ISfinder Mirror Site

ISVba1

Family ISAs1
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAZW01000006	ND	Vibrionales bacterium	Vibrionales bacterium SWAT-3

DNA section

IS Length : 1234 bp

Ends

IR Length : 14/19

IRL : AAGGGCGGGATCGAACTTTAACCATTGAAAATAATGGATTTATTTTACAG
IRR : AAGGGCGCGAATATACTTTGTTAATCTGATGTAAAAAGAACTTTTTCCCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAGATTGCTGGTA	CCCATAACGG	AGCCATGAGCCTT	10

DNA sequence

AAGGGCGGGATCGAACTTTAACCATTGAAAATAATGGATTTATTTTACAGGGAACTTTTTTAGATAATCACGATCTGATCATGAAGTAAAAAAACATGAG
AAATTCGCACATGATTATCATTGAGCAACTTAAAAAAGTGAAAGATACCCGTTCGCACATCAATCAAGTTTATCCTGTTATGGAAGTCGCTTTCGTGGTC
ATAACAGCCATGATTTGTGGTCAAAATAAATGGACAGAGATTAAGGATTTCGGTGAAGGAAATATCGAGTGGTTACGTGAGTATTTCCCCTACGAAAATG
GTCTTCCCACTCGACATAGTATAGCTGCGATCATGAGATCCGTTGTGCCAGAGACGCTATTGGAAGCTATGGTGGGCTGGGTCAATTTGCACAGAGAGAA
GCATGCTCAGCCTATAATCAGTGTTGATGGGAAGGTTCTAAAAGGGGCAAAAGCAAGCAAACAACAGCACCCCCTCTATATGGTTTCGGCATTTGATGTA
GACGAGGGTTTGACTCTCACACACCAACCTTGTGACGGCAAAGGTATGGAGCTGCTAGCAATAAGAAACATGCTAGACGCTTTAGATATCAAAGGATGTT
TATTAACTGCTGATGCCCTGCATTGTCAGGTTGAAACACTGAATAAAGTAGTTGATAAAGGTGGAGATATCCTAGTACAAGTTAAACTGAATCAACCAAG
TTTATTAGCAGAAATTGATGTGCAATTCCAATACTATTGGGCTTTGCCTGAAGATAAACAGGAATCATACATCACGGAAGAGAAAGGGCATGGTAGAAAA
GAGGTTCGTGAGGTTTACGTGCTTCCTGCAGCCTTCAGCGAAGCACTCAGACAGAAGTGGTGTCTCGTAAAAAGCATTGTTGCAGTGGTAAGAGATAGGA
GTGTCAAAGGGAAAGGAAGTTATGAAACCTCGTACTATATTTGCACAGACCATTTATCTTTAGAGTTAGCCTCAAAGGCAACAAGAAAGCATTGGCATAT
TGAGAATCAGCAGCACTGGGCGTTGGACGTAATTTTCAAAGAAGACGAGCAGCGAATTTACGCTGGGGATTCAGCGTTGAATATGGCTTGTTGTCGTCGC
TTTGTGCAGAACCTATTTAGAAAATCAGAGGGTAACTTGTCTGTACCAAGAAAGATGAACCAAGCCGCATGGAATAAAGATTACAGGGAAAAAGTTCTTT
TTACATCAGATTAACAAAGTATATTCGCGCCCTT

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1104 bp	367 aa	111	1214	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MIIIEQLKKVKDTRSHINQVYPVMEVAFVVITAMICGQNKWTEIKDFGEGNIEWLREYFPYENGLPTRHSIAAIMRSVVPETLLEAMVGWVNLHREKHAQ
PIISVDGKVLKGAKASKQQHPLYMVSAFDVDEGLTLTHQPCDGKGMELLAIRNMLDALDIKGCLLTADALHCQVETLNKVVDKGGDILVQVKLNQPSLLA
EIDVQFQYYWALPEDKQESYITEEKGHGRKEVREVYVLPAAFSEALRQKWCLVKSIVAVVRDRSVKGKGSYETSYYICTDHLSLELASKATRKHWHIENQ
QHWALDVIFKEDEQRIYAGDSALNMACCRRFVQNLFRKSEGNLSVPRKMNQAAWNKDYREKVLFTSD

Blast result :

Comments

ISVba1 is 63% aa similar to ISSba1. The ends are not CAGGG as in other ISAs1 family member but AAGGG, this is the cas in all copies n this genome.

References

1] ISfinder annotation (2009)
2] Azam,F., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.