ISfinder Mirror Site

ISThi1

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_011901	ND	Thioalkalivibrio sp.	Thioalkalivibrio sp. HL-EbGR7

DNA section

IS Length : 1191 bp

Ends

IR Length : 11/16

IRL : GGAAACTCTGAAAAAGACTTTCTGATTTGGCAAAATACCGCGACCCCACC
IRR : GGATGGTCTGAAGTAGTTGTGTATTTCTGGCTGACTTCAGCCCCTGCCGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGACAGGCCATAAGGCC	CTAG	AGGACAAGCTTGGG	4

DNA sequence

GGAAACTCTGAAAAAGACTTTCTGATTTGGCAAAATACCGCGACCCCACCCACCGAGTTTCCCGATGAAGCAGATGACCTTCGCCGACGCCGAGTACGCT
GGCAAGCGCAAGCAAACCCGCAAGGAGTTGTTCCTGATCGAGATGGATCGGGTGGTGCCGTGGAAGGGCTTGATTGCTTTGATCGAGCCACATTATCCGA
AGGGTGAAGGTGGCCGTCCGGCCTACCCGTTGATGGCGATGCTGCGTGTGCATCTGCTGCAGAACTGGTTCGGCTACAGCGATCCAGCGATGGAGGAAGC
GCTGTACGAAACCACGATCCTGCGCCAGTTTGCCGGGCTGAACCTGGAGCGCATCCCCGACGAAACCACCATTCTCAACTTCCGCCGCTTGCTGGAGAAA
CACGAGCTGGCGGCCGGCATCCTCGCTGTCATCAATGGCTATCTGGGCGACCGCGGCCTGTCGCTGCGCCAGGGCACCATCGTCGATGCAACGCTGATCA
ATGCGCCCAGTTCGACCAAGAACAAGGACGGCAAGCGCGACCCGGAAATGCACCAGACCAAGAAGGGAAACCAGTATTATTTTGGCATGAAGGCCCACAT
CGGCGCCGATGACGAATCGGGTCTGGTGCACAGCGTAGTGGGCACGGCGGCCAATGTGGCGGATGTCACCCAGGTGGACAAATTGCTGCATGGCGACGAA
AACGTGGTCTGCGCCGATGCAGGCTACACCGGTGTCGAAAAGCGGCCCGAGCATGAAGGACGTGAAGTTATCTGGCAGGTGGCGGCACGCCGCAGCACCT
ACAAAAAACTCGATAAGCGCAGCGTGCTGTACAAAGCCAAGCGCAAGATTGAAAAGGCCAAGGCTCAGGTGCGCGCCAAGGTCGAGCATCCGTTCCGGGT
AATCAAGCGCCAGTTCGGTTACACCAAGGTGCGCTTTCGCGGCTTGGCCAAAAACACGGCGCAACTGGTGACACTGTTCGCTCTGTCGAACCTGTGGATG
GCGCGCCGACATTTACTGGCGAATGCAGGAGAGGTGCGCCTGTAATGTGGAAAATGGCTGCCGCGAGGTGCTCGCGGCGGCTAAAAACACAGAAATAAGC
GGGTGATCTGAGCGTTTTTGATCGATTTGCCGCTTTTAAAATCGGCAGGGGCTGAAGTCAGCCAGAAATACACAACTACTTCAGACCATCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
981 bp	326 aa	65	1045	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKQMTFADAEYAGKRKQTRKELFLIEMDRVVPWKGLIALIEPHYPKGEGGRPAYPLMAMLRVHLLQNWFGYSDPAMEEALYETTILRQFAGLNLERIPDE
TTILNFRRLLEKHELAAGILAVINGYLGDRGLSLRQGTIVDATLINAPSSTKNKDGKRDPEMHQTKKGNQYYFGMKAHIGADDESGLVHSVVGTAANVAD
VTQVDKLLHGDENVVCADAGYTGVEKRPEHEGREVIWQVAARRSTYKKLDKRSVLYKAKRKIEKAKAQVRAKVEHPFRVIKRQFGYTKVRFRGLAKNTAQ
LVTLFALSNLWMARRHLLANAGEVRL

Blast result :

Comments

ISThi1 is 97% aa similar to ISPst7.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Clum,A., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Sorokin,D.Y. and Muyzer,G. (2009) Direct submission GenBank.