ISThsp21
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Thiomonas sp. | Thiomonas sp. 3As |
DNA section
IS Length : 932 bp
Ends
IR Length : 15/17
IRL : GAACTTATCCGTAAATAGATAAAATGGCACTCCGAGTCGCGAGATGCGCG
IRR : GAATCTATCCGTAAATAATATTGACCTGGAGCGGCACCTTCCGGAACGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCTTACCCCTGTTCGAC | TTA | GGAAAGATCTGCACAACCCA | 3 |
DNA sequence
GAACTTATCCGTAAATAGATAAAATGGCACTCCGAGTCGCGAGATGCGCGGCGTCGGAGCAATGCATTGGCCAAGCGAGCGGAGTCGTGGGTCGTGACGG
ATGCATTCTGGCAACGGGCGGAGCCTTTGGTTCCACAGCGGGTCGCGCCGGTCGACAGGGTGTATAAGCGCAGGCCCGGGGCGGGGCGACCGCCGAAGCC
AGCGCGGCAGGTGTTTGAGGCGATTGTGTACGTGCTGCGCACGGGCTGTCAGTGGAAGGCGTTGCCCAAGGAGCGATTTGGCAGTGCCAGCGCCGTGCAC
AAGCGCTTCCTGGAGTGGGAAAAGGCAGGTTTCTTCGAGGCGCTGTGGAAGGCCGGCTTGGCCGAGTACGACGACATGCAAGGCATTGCGTGGCGATGGC
AGAGCGTGGACGGCGCGATGACGCGTGCGCCGCTGGCACAGCAATCCGTCGGGCCAAACCCGACGGATCGGGGGAAAAAATGGGAGCAAGCGTCACCTGC
TGGTGGACGGCCGTGGCGTCCCGTTGTTGCTCATCGTGACCGGGGCGAACATCAATGACTGCAAGCGCCTGGACGAGGTGCTGAGCGCGATCATGGTCAA
GCGCGAGAGCCCACCGTCGCGGCGCCACAAGCACCTGTGCGCAGACGCCGGCTATCGCGGTGCCGACAACGCTCGCACCATCGAAAAGCATGACAACATC
GCCCACGTCGTCAGCCGCCGCAAAGAGGCAGACATCAAGCGGCGTGACCCGACGAAGAAGGCGCGACGTTGGGTCGTCGAGGTCTGCCATAGCTGGTTCA
ACCGGTTGCGCAAGCTGCTGGTGCGCTACGAGAAGCTCGAGCGCAGTTTCGTCGCGCTCAACCACCTCGCCGCAGCGATCATTGCGTTCCGGAAGGTGCC
GCTCCAGGTCAATATTATTTACGGATAGATTC
ATGCATTCTGGCAACGGGCGGAGCCTTTGGTTCCACAGCGGGTCGCGCCGGTCGACAGGGTGTATAAGCGCAGGCCCGGGGCGGGGCGACCGCCGAAGCC
AGCGCGGCAGGTGTTTGAGGCGATTGTGTACGTGCTGCGCACGGGCTGTCAGTGGAAGGCGTTGCCCAAGGAGCGATTTGGCAGTGCCAGCGCCGTGCAC
AAGCGCTTCCTGGAGTGGGAAAAGGCAGGTTTCTTCGAGGCGCTGTGGAAGGCCGGCTTGGCCGAGTACGACGACATGCAAGGCATTGCGTGGCGATGGC
AGAGCGTGGACGGCGCGATGACGCGTGCGCCGCTGGCACAGCAATCCGTCGGGCCAAACCCGACGGATCGGGGGAAAAAATGGGAGCAAGCGTCACCTGC
TGGTGGACGGCCGTGGCGTCCCGTTGTTGCTCATCGTGACCGGGGCGAACATCAATGACTGCAAGCGCCTGGACGAGGTGCTGAGCGCGATCATGGTCAA
GCGCGAGAGCCCACCGTCGCGGCGCCACAAGCACCTGTGCGCAGACGCCGGCTATCGCGGTGCCGACAACGCTCGCACCATCGAAAAGCATGACAACATC
GCCCACGTCGTCAGCCGCCGCAAAGAGGCAGACATCAAGCGGCGTGACCCGACGAAGAAGGCGCGACGTTGGGTCGTCGAGGTCTGCCATAGCTGGTTCA
ACCGGTTGCGCAAGCTGCTGGTGCGCTACGAGAAGCTCGAGCGCAGTTTCGTCGCGCTCAACCACCTCGCCGCAGCGATCATTGCGTTCCGGAAGGTGCC
GCTCCAGGTCAATATTATTTACGGATAGATTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 492 bp | 163 aa | 67 | 558 | + | No |
Description : First part of the transposase
ORF sequence :
LAKRAESWVVTDAFWQRAEPLVPQRVAPVDRVYKRRPGAGRPPKPARQVFEAIVYVLRTGCQWKALPKERFGSASAVHKRFLEWEKAGFFEALWKAGLAE
YDDMQGIAWRWQSVDGAMTRAPLAQQSVGPNPTDRGKKWEQASPAGGRPWRPVVAHRDRGEHQ
YDDMQGIAWRWQSVDGAMTRAPLAQQSVGPNPTDRGKKWEQASPAGGRPWRPVVAHRDRGEHQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 507 bp | 168 aa | 422 | 928 | + | No |
Description : Second part of the transposase
ORF sequence :
RVRRWHSNPSGQTRRIGGKNGSKRHLLVDGRGVPLLLIVTGANINDCKRLDEVLSAIMVKRESPPSRRHKHLCADAGYRGADNARTIEKHDNIAHVVSRR
KEADIKRRDPTKKARRWVVEVCHSWFNRLRKLLVRYEKLERSFVALNHLAAAIIAFRKVPLQVNIIYG
KEADIKRRDPTKKARRWVVEVCHSWFNRLRKLLVRYEKLERSFVALNHLAAAIIAFRKVPLQVNIIYG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 862 bp | 287 aa | 67 | 928 | + | Yes |
Chemistry : DDE
ORF sequence :
LAKRAESWVVTDAFWQRAEPLVPQRVAPVDRVYKRRPGAGRPPKPARQVFEAIVYVLRTGCQWKALPKERFGSASAVHKRFLEWEKAGFFEALWKAGLAE
YDDMQGIAWRWQSVDGAMTRAPLAQQSVGPNPTDRGKKNGSKRHLLVDGRGVPLLLIVTGANINDCKRLDEVLSAIMVKRESPPSRRHKHLCADAGYRGA
DNARTIEKHDNIAHVVSRRKEADIKRRDPTKKARRWVVEVCHSWFNRLRKLLVRYEKLERSFVALNHLAAAIIAFRKVPLQVNIIYG
YDDMQGIAWRWQSVDGAMTRAPLAQQSVGPNPTDRGKKNGSKRHLLVDGRGVPLLLIVTGANINDCKRLDEVLSAIMVKRESPPSRRHKHLCADAGYRGA
DNARTIEKHDNIAHVVSRRKEADIKRRDPTKKARRWVVEVCHSWFNRLRKLLVRYEKLERSFVALNHLAAAIIAFRKVPLQVNIIYG
Blast result :
Comments
ISThsp21 is 92% aa similar to ISCARN38. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)