ISThsp20
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Thiomonas sp. | Thiomonas sp. 3As |
DNA section
IS Length : 833 bp
Ends
IR Length : 17/19
IRL : GATAGTGTCGTCACGAGATATGGCATGATGGCGTTGTTCGCAACGACTGC
IRR : GGTCGTGTCGTCACGAGATGCGCAGCCAGAGGACGATGCATCGAATTTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCATGCGGAACCC | TA | GCCTGATGCTGGT | 2 |
DNA sequence
GATAGTGTCGTCACGAGATATGGCATGATGGCGTTGTTCGCAACGACTGCGCTATTGAGATGCCAGAGACTCTTCATCGACACGACCTATCAGACAGGCA
CTGGGAATTGCTCGAGCCCCATCTGCCGGGGCGAGCGGGGGTTTGGGGTGGGGTTGCGCAGGACAACCGCCAATTCATCAATGCCGTGTTCTGGATTCTT
CGCACCGGCGCGCCGTGGCGAGACCTGCCGGCCTCGTATGGTGGATGGAAAAACACCCATCGGCGTTTTTGCCGCTGGCGTGACCAGGGCATTTGGGAGT
CGCTGCTCGAGCAGTTCATTGTGGAGCCTGATTTCGAATGGCTCATGATGGATGCCAGCCATTGCAAAGTCCATCCACACGCCGCAGGAGCGCGGGGTGG
CAATCAGGCCATGAGCCGTACAAAAGGGGGCTCAACACCAAGATACATCTGGCCGTGGATGCGCATGGTCTGCCGCTCAGAGCGGTTATTACAGAAGGTG
CCCGAGCGGATTGCGCAGAGGCTGAGCGGTTGATGGATGGATTCAGGATGCAGTGTCTACTTGCCGATAAAGGCTACGACAGCGATGCCATCGTGGCGAA
AGCCCAAGAGCAAGGTGCGCAAGCCGTCATTCCGTCGCGCAAGAACCGCAAGCAACCCAGGGCCTATGACAAGGCGCTCTACAAACTGCGCCATCTGGTC
GAGAACGCCTTCCTACATCTCAAGCGATGGAGGGGCATCGCCACGCGCTACGCGAAAAACGTCGCCTCATTCGTTGCCGCCGTGCAAATTCGATGCATCG
TCCTCTGGCTGCGCATCTCGTGACGACACGACC
CTGGGAATTGCTCGAGCCCCATCTGCCGGGGCGAGCGGGGGTTTGGGGTGGGGTTGCGCAGGACAACCGCCAATTCATCAATGCCGTGTTCTGGATTCTT
CGCACCGGCGCGCCGTGGCGAGACCTGCCGGCCTCGTATGGTGGATGGAAAAACACCCATCGGCGTTTTTGCCGCTGGCGTGACCAGGGCATTTGGGAGT
CGCTGCTCGAGCAGTTCATTGTGGAGCCTGATTTCGAATGGCTCATGATGGATGCCAGCCATTGCAAAGTCCATCCACACGCCGCAGGAGCGCGGGGTGG
CAATCAGGCCATGAGCCGTACAAAAGGGGGCTCAACACCAAGATACATCTGGCCGTGGATGCGCATGGTCTGCCGCTCAGAGCGGTTATTACAGAAGGTG
CCCGAGCGGATTGCGCAGAGGCTGAGCGGTTGATGGATGGATTCAGGATGCAGTGTCTACTTGCCGATAAAGGCTACGACAGCGATGCCATCGTGGCGAA
AGCCCAAGAGCAAGGTGCGCAAGCCGTCATTCCGTCGCGCAAGAACCGCAAGCAACCCAGGGCCTATGACAAGGCGCTCTACAAACTGCGCCATCTGGTC
GAGAACGCCTTCCTACATCTCAAGCGATGGAGGGGCATCGCCACGCGCTACGCGAAAAACGTCGCCTCATTCGTTGCCGCCGTGCAAATTCGATGCATCG
TCCTCTGGCTGCGCATCTCGTGACGACACGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 474 bp | 157 aa | 60 | 533 | + | No |
Description : First part of the transposase
ORF sequence :
MPETLHRHDLSDRHWELLEPHLPGRAGVWGGVAQDNRQFINAVFWILRTGAPWRDLPASYGGWKNTHRRFCRWRDQGIWESLLEQFIVEPDFEWLMMDAS
HCKVHPHAAGARGGNQAMSRTKGGSTPRYIWPWMRMVCRSERLLQKVPERIAQRLSG
HCKVHPHAAGARGGNQAMSRTKGGSTPRYIWPWMRMVCRSERLLQKVPERIAQRLSG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 492 bp | 163 aa | 332 | 823 | + | No |
Description : Second part of the transposase
ORF sequence :
FRMAHDGCQPLQSPSTRRRSAGWQSGHEPYKRGLNTKIHLAVDAHGLPLRAVITEGARADCAEAERLMDGFRMQCLLADKGYDSDAIVAKAQEQGAQAVI
PSRKNRKQPRAYDKALYKLRHLVENAFLHLKRWRGIATRYAKNVASFVAAVQIRCIVLWLRIS
PSRKNRKQPRAYDKALYKLRHLVENAFLHLKRWRGIATRYAKNVASFVAAVQIRCIVLWLRIS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 764 bp | 254 aa | 60 | 823 | + | Yes |
Chemistry : DDE
ORF sequence :
MPETLHRHDLSDRHWELLEPHLPGRAGVWGGVAQDNRQFINAVFWILRTGAPWRDLPASYGGWKNTHRRFCRWRDQGIWESLLEQFIVEPDFEWLMMDAS
HCKVHPHAAGARGGNQAMSRTKGGLNTKIHLAVDAHGLPLRAVITEGARADCAEAERLMDGFRMQCLLADKGYDSDAIVAKAQEQGAQAVIPSRKNRKQP
RAYDKALYKLRHLVENAFLHLKRWRGIATRYAKNVASFVAAVQIRCIVLWLRIS
HCKVHPHAAGARGGNQAMSRTKGGLNTKIHLAVDAHGLPLRAVITEGARADCAEAERLMDGFRMQCLLADKGYDSDAIVAKAQEQGAQAVIPSRKNRKQP
RAYDKALYKLRHLVENAFLHLKRWRGIATRYAKNVASFVAAVQIRCIVLWLRIS
Blast result :
Comments
ISThsp20 is 94% aa similar to ISThsp6. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)