ISfinder Mirror Site

ISBaps1

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_ACMX01000030	ND	Bacillus pseudomycoides	Bacillus pseudomycoides DSM 12442

DNA section

IS Length : 2424 bp

Ends

IR Length : 7/15

IRL : GTAAGCGTCAAACATTCCCCACTTACAATTTGTAAATAACCCATGAGATA
IRR : GTAACTCCAAAATATACAATAAAAGTTGATTTTGGAGTTATTTTTTGTTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TACCAGCTTCTTT		TTTACGTTTACTT	0

DNA sequence

GTAAGCGTCAAACATTCCCCACTTACAATTTGTAAATAACCCATGAGATAATCTCCGTACATACTACATGTGGAGGTTTTTTTTATGAAAAGACACCTAT
TAAAGAAAGAATGGGAGACTAATATTCAAGACTATAAAAACAGCGGATTATCAAAAGTTGCCTGGTGTCAGAAACAGAACCTACGGGTTCATCGGCTATA
TTACTGGCTAAACAAATTATCACCTGAAACGGAAGATCAAAATAAAAATGAGCGTGTGAATTGGATTTCTATGAATGTCCCTGTCGTAGAAGAAAACATA
CATGCCACCATTCGAATTCACATGCAAGAAGCCACTATTGAGTTAGATGTGCCTTTTACTCCACAAGTGCTCCTACAAGTGATACAGACGGTGAAACAAC
AATGATATCCGAAATCGCAATTGAAAAAGTATATCTTGCACAAGGGGCAACAGATTTACGTAAATCAATTGATGGACTAGCAGCTATTGTAAAGGAAGAA
TTTGAGTTAGACCCTTTTTCTTCTTGTTTATTTGTTTTTTGTAATCGCTCACGCGATAAACTAAAAATTCTTGTTTGGGAACATAATGGTTTCTGGTTGC
ACTATCGCAGATTGGAAAAGGGAACGTTTTACTGGCCAACTGGTCATCCGGGTGTGCCACTTACCATTTCAAGACGACAATTGAGATGGCTTCTTGATGG
ATTTGCTTTTCAACAAAAACAAGGTCATCAAGCCGTACATGCGAGAACCATTCTATAAAAATCATATATGAAAAAGGAATTTGGATGATTTTATCGAATG
GATTTATCTATGAAAACAACACTCTCACATTCAAAACAACCCACAATTGAATCACTTCAAGCTCAAGTAGAGGAACTCACGGAAAAAGTGAGATGGTATG
AAGAACAATTTCGTCTTAGTCAACAAAAACGGTTTGGTACGTCTAGCGAAAAAACACCTAATAATCAACTCGCTTTAGAACTGTTTAATGAGGCTGAGAA
AGAAAGTGACTCTGAAACCCCTGAATCAGCTGTTGAAACAATCACCTATCGTCGTAAGAAGAAACACGGTCATAGGGATGAGTCTGTCCAAAACCTTCCG
GTTGAAACGATTGAATATCGTTTACTTGATATGGAACAGGTTTGTTTGTGCTGTGGTGGTGCTTTGCATGAGATGAGTGTAGAAGTCCGTAAGGAACTGA
CGATTATCCCTGCGGAAGTAAAGGTGACGGAACATAAGAGGTATGTATACGCTTGTCGTAAGTGTGAATTAGATGAGGTATCTACACCGATTGTGACGGC
TAAGATGCCAGCCCCTGCCTTTCCTAAAAGTATCGCTTCCCCATCGATTATGGCGTATATCATGACACAAAAATATGTAGAAGGATTGCCTCTTTATCGT
CAGAAAAAGCATTTTAAACGAATGGAAATCTTCTTATCACGACAAACGATGGGAAATTGGTTATTATATGGGGCGGATCGATGGTTAGTGGCTTTGTATG
AAAGGATGCATGAACATTTACTTACTCGAACCATTCTTCATGCGGACGAAACAACCTTCCAAGTCTTACGCGTACCTGGTCGAGCCGCAACAACCAAGTC
CTATCTGTGGTTATATCGTACAGGACGAGAGGATATCCCTATTGTCCTCTATGACTATCAACCTACAAGAGCAGGCGAACATCCGAAACGTTTTTTAACT
GGTTTTGAAGGATATTTACAGGTAGATGGATATAGTGGTTATCATCAAGTACCGGATGTCACCCTTATCGGATGTTGGGCGCATGCGAGACGAAAGTTTG
ATGAAGCCTTAAAGGCTTTACCTGATTCACAGAAGGGGAAAAAAGTGAAGGCGAGCGAAGGTTTACACTTCTGTAATCAACTCTATTCGATTGAAAGGAA
GCTCAGACATGTCAACCCTACCGAGCGATATGAGCAGAGACTGAAAAAAAGCAGGCCCATTCTGAACCTTTTTTCAGCATGGCTTCATGAACAGAAAGAT
CGTGTTTTACCAAAAAGTGCGCTAGGCAAAGCCATCACTTATTGTTTGAATCAACGGAATCATCTTGAAGCTTTTTTATTAGACGGTCGTTTAGAAATCG
ATAACAACAGAAGTGAACGTTCTATTAAACCGTTTGTAATCGGGAGAAAAAATTGGATGTTCTCAAATACACCGCGAGGTGCCAGAGGAAGTGCCATCAT
GTATAGTGTGGTGGAAACTGCAAAAGAGAATGGTTTAAGTCCTTATCATTATCTTCGTTATTTATTTGAAACACTTCCCAACATCGATTTAAACAATAAA
GAGGAACTGGATAAAATCTTGCCGTGGTCAGCGGATTTACCATCTAGCTGTAGAGTGCCGAAAAAAAGTGAAGTAAACAAAAAATAACTCCAAAATCAAC
TTTTATTGTATATTTTGGAGTTAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
321 bp	106 aa	85	405	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MKRHLLKKEWETNIQDYKNSGLSKVAWCQKQNLRVHRLYYWLNKLSPETEDQNKNERVNWISMNVPVVEENIHATIRIHMQEATIELDVPFTPQVLLQVI
QTVKQQ

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
357 bp	118 aa	402	758	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MISEIAIEKVYLAQGATDLRKSIDGLAAIVKEEFELDPFSSCLFVFCNRSRDKLKILVWEHNGFWLHYRRLEKGTFYWPTGHPGVPLTISRRQLRWLLDG
FAFQQKQGHQAVHARTIL

Blast result :

Comments

Genome in progress. Accession number: NZ_ACMX01000030.
ISBaps1 is 47%(orfA) aa similar to ISCce5, 68%(orfB) to ISSba7, and 63%(orfC) to ISCce5.

References

1] ISfinder annotation (2009).
2]Read,T.D., Akmal,A., Bishop-Lilly,K., Chen,P.E., Cook,C., Kiley,M.P., Lentz,S., Mateczun,A., Nagarajan,N., Nolan,N., Osborne,B.I., Pop,M., Sozhamannan,S., Stewart,A.C., Sulakvelidze,A., Thomason,B., Willner,K. and Zwick,M.E. (2009) Direct submission GenBank.