ISfinder Mirror Site

ISSpi3

Family IS110
Group IS1111

MGE type ISRelated element(s) :
Isoform Synonym(s) ISswpIII

Accession number	Transposition	Origin	Host
NC_011566	ND	Shewanella piezotolerans	Shewanella piezotolerans WP2

DNA section

IS Length : 1593 bp

Ends

IR Length : 12/12

IRL : -tagacataATGACTCCCCACTAAAGGTGCTGATCTCCAATCAATCGTGG
IRR : ggcgttggtATGACTCCCCACGTCAAGCAGAACATCACTATTTCGGCCTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGACACCTTA		CTAATAATGG	0
TCCTTGTACA		CTAATAATGG	0
CTTAGCAACA		CTAATAATGG	0

DNA sequence

TAGACATAATGACTCCCCACTAAAGGTGCTGATCTCCAATCAATCGTGGGTTAGCTGAAAGCCAGAGCCAGAATAGTTTTGTTAAAGAAACTAAATCGGA
GATCAGCATGAATAAACATAACATACTTTTTATTGGCCTTGATACACATAAAGAATTTGTAGAAATTGCTTATATTGAAGATCAAAGAGGAGCGCAAGCG
GTTCATTTTGGACGAGTTTCAAGTGCGAAAGCCTCTATCACTAGACTGGCTAGGCAGTTTCAATCTAAGTACCCAGAAGCAACCTTACACTTTGTGTATG
AGGCTGGCCCCTGCGGTTACTGGATCTACCGATTGCTTACCAGCCTAGGTCACTGTTGCTATGTTATAGCCCCTTCACTTATTCCCAAAAAACCTGGTGA
GAAAATTAAAACCGATAAACGTGATGCGCTTAAATTAGCAAGGCTGCTTAAGTCGGAAGATCTTACCGCGATTTATGTTCCAGAGCCTGAAGATGAAGCT
ATCCGCGATTTATCTCGTGCTCGTGAGACCGCGATGAAAGATTTAAAAGACGCTAAATACCAGCTTAAAGCGCTTCAGCTAAGAAACAATATTAACTACA
GAGGCACTGCTAACTGGTCGCTAAAGCACTTACGCTGGCTGACTGAACTGATATTGCCGCACCCGAGTCAACAGATTGTCTTGCAAGAATACTTACAAAC
CATTTCAGAGCGGATCGCGAGGCTAAAACGGCTAGACAATGAACTTGAGCATCACGTTAAAAAGTGGCGTTAATATCCGGTCATCAAAGCCATACAAGCA
TTACGTGGAGTGAGGTTACTGGTTGCTGCAGGTGTCATTGCTGAGTTGGGAGACTTAACTCGCTTTGATCATCCCCGAAAACTAATGAGTTATGTAGGCC
TAGTTCCATCGGAACACTCAAGTGGTGGAAAGCGGCATGTGGGTGCGATAACGAAATGCGGCAATGGTCGAGCAAGACGGCTATTAGTCGAAGGGGCTCA
CTCCTATCGCTACCCCGCTAAGATCTCAACCGAGATGCAAATAAGACAAGAAGGTTTACCGAAAGAAATCATTGATATTGCATGGCAAGCGCAGCTTAGG
CTTTGTCGTCGATACCAAAAACTCATGTACAAAGGTAAACATAACAACGTAGTCGTGACAGCCATAGCCAGAGAAATGATTGCTTATATTTGGGCGATTT
CACGAGAGGTGGTACTGACAAAAGTTAACCCTAAACTACGGTTGTCGAGAGTACCTGCATGATAAACGATTTTGAGCTAATACAGTGGGTCAAGCATCGG
ATGTGACACGATCACCGAGGGCGTTAGGATGGCAATACGGTTAACTCTGTATTGAACCACGAACATAGACTGAAGACAGGTGTCACGACGCCATAATTAA
TAACAAAAGGTAAGGTAGGCGCTGCTTATTGAAAGATAAGTAATCCACGGATATCAGCATGACAACCGACGAAATTACTTGCTTCATCGACTGTGTTAGC
TCAATCCAATTTAGTCAAAAGAGCAAAAGCAGGCTTAAATATTTAGGCCGAAATAGTGATGTTCTGCTTGACGTGGGGAGTCATACCAACGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1155 bp	384 aa	108	1262	+	No

ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MNKHNILFIGLDTHKEFVEIAYIEDQRGAQAVHFGRVSSAKASITRLARQFQSKYPEATLHFVYEAGPCGYWIYRLLTSLGHCCYVIAPSLIPKKPGEKI
KTDKRDALKLARLLKSEDLTAIYVPEPEDEAIRDLSRARETAMKDLKDAKYQLKALQLRNNINYRGTANWSLKHLRWLTELILPHPSQQIVLQEYLQTIS
ERIARLKRLDNELEHHVKKWRXYPVIKAIQALRGVRLLVAAGVIAELGDLTRFDHPRKLMSYVGLVPSEHSSGGKRHVGAITKCGNGRARRLLVEGAHSY
RYPAKISTEMQIRQEGLPKEIIDIAWQAQLRLCRRYQKLMYKGKHNNVVVTAIAREMIAYIWAISREVVLTKVNPKLRLSRVPA

Blast result :

Comments

There are 4 copies of ISSpi3 encoded in the Shewanella piezotolerans genome. The transposases encoded by all 4 are degenerate. The transposase deposited here only has a point mutation and has 72% identity to that encoded by ISVisp2. The terminal inverted repeat starts 8 bp from the 5' end and 9 bp from the 3' end.

References

1] Romine, M. (2008) Direct submission.
2] Wang,F., Wang,J., Jian,H., Zhang,B., Li,S., Wang,F., Zeng,X., Gao,L., Bartlett,D.H., Yu,J., Hu,S. and Xiao,X. (2008) PLoS ONE 3 (4), E1937