ISSpi1
- Family IS3
- Group IS51
Isoform Synonym(s) ISSwp1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011566 | ND | Shewanella piezotolerans | Shewanella piezotolerans WP3 |
DNA section
IS Length : 1234 bp
Ends
IR Length : 23/28
IRL : TGGTAAGCCCCTAGAACAGTAGACACTTCATAGAGTTATACTTAACCAAA
IRR : TGGTAAGCCCCTAGTTTTCTAGACACTTTCTAAGTTCTCATAATACTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATTAAGTTC | AAC | CCTACACAAC | 3 |
| TTCAGCGGCC | ACC | ACTTTATTCA | 3 |
| CAATATTCGA | TCT | GTCTGGGTGA | 3 |
| TATCCAGCTG | CGT | TCTTATCGGG | 3 |
| CCTTCAAAGT | ACC | TCTCCCGTGG | 3 |
DNA sequence
TGGTAAGCCCCTAGAACAGTAGACACTTCATAGAGTTATACTTAACCAAAAATATGAGGTGTTACATGCGCGGAAAACGATATCCAGATGAGTTCAAAAT
AGAAGCTGTTAAACAAGTCACTGAGCGTGGTTATCAAATCGCAGATGTTGCTGATAGATTAGGCGTCACTTCCAAAAGTTTGCACAACTGGATTAATAAG
TTTGATAAGCCGGAAAAGCAACACATCACCATTGATAATCAACAAGACGAAATACGTAAACTCAAGGCTGAACTACGTCGCGTAACCGAAGAGCGAAATA
TCCTAAAGGAGGCCGCCGTGTACTTTGCAAGCGAGTCAAAGAAAAGTACACGTTCATAAAGTCTAGGCTCAAGCACTACCCAGTAAAAACCCTATGCGAA
ATACTTGGCGTTCACCGCAGCGGCTTCTATGCCTGGCTTAAGGAGCCTCTGAGTCGCAGAGCTACTGAAGATAAGCGACTACTAGGTAAAATTAAACAGT
ATTGGCTAGAAAGCGGTTGTGTGTATGGCTACCGAAATATCACTTTAGACCTTAAAGATGATGGTGAGGCTGTTGGAAAAAATCGGGTATATCGGATCAT
GAGAACAGCTGATATTAAGGCTGTACGTGGCTATAAGCGCAATCCAAGTTTTGGCAGAGGCGATGTTAGTCATACAGCACCAAATACGTTAAATAGAGGG
TTTGACATCGCTGAGCCCAATAAAGTTTGGGTGACCGACTTTACCTATATTCGGACTCATGAAGGTTGGCTATATCTCACTGTTGTTATCGATCTATTCT
CTCGACAAGTTGTCGGTTGGACAATGAAAAGTACAGCTAGAGCTGACCTAGTTATTGATGCGCTACTGATGGCGGTATGGAGACGCACTCCAACAGAAAA
GGTGCTTATCCATTCCGATCAAGGCGTGCAATATACTTGCTCTGATTGGCGCAGCTTTCTTAAAGAGCACAACCTGGAAGCGAGTATGAGTCGAAGAGGA
AACTGTCATGATAATGCAGTTGCTGAAAGCTTTTTCTCATTACTGAAAAAAGACAGGATTAAGCGAAAAGTCTACAAAACCAGAGATGAAGCGCGTGCTG
AAATATTTAACTATATCGAATATTTCTACAATCCAGTGCGGCATCATGGTAGTAATAACGGACTGTCTCCAATGAATTTTGAAAAGCAGTATTATGAGAA
CTTAGAAAGTGTCTAGAAAACTAGGGGCTTACCA
AGAAGCTGTTAAACAAGTCACTGAGCGTGGTTATCAAATCGCAGATGTTGCTGATAGATTAGGCGTCACTTCCAAAAGTTTGCACAACTGGATTAATAAG
TTTGATAAGCCGGAAAAGCAACACATCACCATTGATAATCAACAAGACGAAATACGTAAACTCAAGGCTGAACTACGTCGCGTAACCGAAGAGCGAAATA
TCCTAAAGGAGGCCGCCGTGTACTTTGCAAGCGAGTCAAAGAAAAGTACACGTTCATAAAGTCTAGGCTCAAGCACTACCCAGTAAAAACCCTATGCGAA
ATACTTGGCGTTCACCGCAGCGGCTTCTATGCCTGGCTTAAGGAGCCTCTGAGTCGCAGAGCTACTGAAGATAAGCGACTACTAGGTAAAATTAAACAGT
ATTGGCTAGAAAGCGGTTGTGTGTATGGCTACCGAAATATCACTTTAGACCTTAAAGATGATGGTGAGGCTGTTGGAAAAAATCGGGTATATCGGATCAT
GAGAACAGCTGATATTAAGGCTGTACGTGGCTATAAGCGCAATCCAAGTTTTGGCAGAGGCGATGTTAGTCATACAGCACCAAATACGTTAAATAGAGGG
TTTGACATCGCTGAGCCCAATAAAGTTTGGGTGACCGACTTTACCTATATTCGGACTCATGAAGGTTGGCTATATCTCACTGTTGTTATCGATCTATTCT
CTCGACAAGTTGTCGGTTGGACAATGAAAAGTACAGCTAGAGCTGACCTAGTTATTGATGCGCTACTGATGGCGGTATGGAGACGCACTCCAACAGAAAA
GGTGCTTATCCATTCCGATCAAGGCGTGCAATATACTTGCTCTGATTGGCGCAGCTTTCTTAAAGAGCACAACCTGGAAGCGAGTATGAGTCGAAGAGGA
AACTGTCATGATAATGCAGTTGCTGAAAGCTTTTTCTCATTACTGAAAAAAGACAGGATTAAGCGAAAAGTCTACAAAACCAGAGATGAAGCGCGTGCTG
AAATATTTAACTATATCGAATATTTCTACAATCCAGTGCGGCATCATGGTAGTAATAACGGACTGTCTCCAATGAATTTTGAAAAGCAGTATTATGAGAA
CTTAGAAAGTGTCTAGAAAACTAGGGGCTTACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 66 | 359 | + | No |
Description : First part of the transposase
ORF sequence :
MRGKRYPDEFKIEAVKQVTERGYQIADVADRLGVTSKSLHNWINKFDKPEKQHITIDNQQDEIRKLKAELRRVTEERNILKEAAVYFASESKKSTRS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 945 bp | 314 aa | 272 | 1216 | + | No |
Description : Second part of the transposase
ORF sequence :
TTSRNRRAKYPKGGRRVLCKRVKEKYTFIKSRLKHYPVKTLCEILGVHRSGFYAWLKEPLSRRATEDKRLLGKIKQYWLESGCVYGYRNITLDLKDDGEA
VGKNRVYRIMRTADIKAVRGYKRNPSFGRGDVSHTAPNTLNRGFDIAEPNKVWVTDFTYIRTHEGWLYLTVVIDLFSRQVVGWTMKSTARADLVIDALLM
AVWRRTPTEKVLIHSDQGVQYTCSDWRSFLKEHNLEASMSRRGNCHDNAVAESFFSLLKKDRIKRKVYKTRDEARAEIFNYIEYFYNPVRHHGSNNGLSP
MNFEKQYYENLESV
VGKNRVYRIMRTADIKAVRGYKRNPSFGRGDVSHTAPNTLNRGFDIAEPNKVWVTDFTYIRTHEGWLYLTVVIDLFSRQVVGWTMKSTARADLVIDALLM
AVWRRTPTEKVLIHSDQGVQYTCSDWRSFLKEHNLEASMSRRGNCHDNAVAESFFSLLKKDRIKRKVYKTRDEARAEIFNYIEYFYNPVRHHGSNNGLSP
MNFEKQYYENLESV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 66 | 1216 | + | Yes |
Chemistry : DDE
ORF sequence :
MRGKRYPDEFKIEAVKQVTERGYQIADVADRLGVTSKSLHNWINKFDKPEKQHITIDNQQDEIRKLKAELRRVTEERNILKGGRRVLCKRVKEKYTFIKS
RLKHYPVKTLCEILGVHRSGFYAWLKEPLSRRATEDKRLLGKIKQYWLESGCVYGYRNITLDLKDDGEAVGKNRVYRIMRTADIKAVRGYKRNPSFGRGD
VSHTAPNTLNRGFDIAEPNKVWVTDFTYIRTHEGWLYLTVVIDLFSRQVVGWTMKSTARADLVIDALLMAVWRRTPTEKVLIHSDQGVQYTCSDWRSFLK
EHNLEASMSRRGNCHDNAVAESFFSLLKKDRIKRKVYKTRDEARAEIFNYIEYFYNPVRHHGSNNGLSPMNFEKQYYENLESV
RLKHYPVKTLCEILGVHRSGFYAWLKEPLSRRATEDKRLLGKIKQYWLESGCVYGYRNITLDLKDDGEAVGKNRVYRIMRTADIKAVRGYKRNPSFGRGD
VSHTAPNTLNRGFDIAEPNKVWVTDFTYIRTHEGWLYLTVVIDLFSRQVVGWTMKSTARADLVIDALLMAVWRRTPTEKVLIHSDQGVQYTCSDWRSFLK
EHNLEASMSRRGNCHDNAVAESFFSLLKKDRIKRKVYKTRDEARAEIFNYIEYFYNPVRHHGSNNGLSPMNFEKQYYENLESV
Blast result :
Comments
There are 5 copies of ISSpi1 encoded in Shewanella piezotolerans (one is degenerate, having a frameshift in the transposase encoding gene). The transposase encoded by ISSpi1 has 92% identity to that encoded by ISSse2. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift
References
1] Romine, M. (2008) Direct submission.
2] Wang,F., Wang,J., Jian,H., Zhang,B., Li,S., Wang,F., Zeng,X., Gao,L., Bartlett,D.H., Yu,J., Hu,S. and Xiao,X. (2008) PLoS ONE 3 (4), E1937
2] Wang,F., Wang,J., Jian,H., Zhang,B., Li,S., Wang,F., Zeng,X., Gao,L., Bartlett,D.H., Yu,J., Hu,S. and Xiao,X. (2008) PLoS ONE 3 (4), E1937