ISfinder Mirror Site

ISPtu2

Family IS110
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Pseudoalteromonas tunicata	Pseudoalteromonas tunicata

DNA section

IS Length : 1201 bp

Ends

Left end : ATGGATAATAAACCCATCAAATTATTCTTTTAAACCGAGTTCGGGTAATGTGAGACCCAGGCTTTAGCTGCAACTAAAGCTCTCTTTTGTGGTAGTTCGA
Right end : GTGTGTCAGGAAGATGATTGTGATCTTAAATTCGATGCTCAGAGACGGAGTAATGTGGGAAGCGCCAAAAGTTTAAAATTAGCTATTGACGCCATAGTCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTTTAAACGA	CTTGT	TATACGTTTT	5
GATTGAGCAG	CTTGT	TAGCTTGCCT	5
TTTTAAACGA	CTTGT	TAACTGCCAA	5

DNA sequence

ATGGATAATAAACCCATCAAATTATTCTTTTAAACCGAGTTCGGGTAATGTGAGACCCAGGCTTTAGCTGCAACTAAAGCTCTCTTTTGTGGTAGTTCGA
TTGAGCGATGGATCCTCTGTTGAGAGACCGATAACAAGAATGAACGCCACAAAAGACTCCAAGCATCACACTCGAATAGTCTACTGGGTCTCGAACCCGC
ATTATGCAAGCAAGAGTTAGCTTATTATGAAAACAAATACTAATCAAAACATTAATGTCGGTGTCGATACCGGCAAATTTCAACTCGATATTTATATTCG
CCCTCTTGATATCTATTTCACCGTTAAGAACGATGAAAAAGGGATTGCAGAGGCTATTAAAAAAATCAAAATTCACTCTCCAAACCGCATCATTATTGAA
GCCACTGGCCGCCTTGAAATGCCCTTTATTATTGCATGTGCAAAGGCCAATTTACCCTTTGTCATTGCCAACCCAATACACATAAAACGCTTTGCTGGCG
CCATTGGTCAACGCGCTAAAACCGATAAGCTAGATGCACAGTTAATTGCACATTATGGCGAGGCAATTAAACCCACACTCTCCACCCTAAAGCCTGACAT
TATGCAGACTATGAGTGATTTGGTTGCCAGAAGAAATCAGCTGTTAGTCATGCAAACGATGGAAAAAAACCGCCTGCAAATTTTACCAAAAGAGCTGATT
TCAACCATCAAACCTATTTTAACGGCCTTTAAAAATCAGATTAAAAAAATTGAAAATAAAATTCTAAAACTCATTGAATCATGCCCTGAATATTCAGCCA
AAAACACGATCCTGCAAAGCATGAAAGGCATTGGTAAAATTGCCGCTGCCTCCATCATCAGTAACTTACCTGAGCTTGGCTATATGTCGAATAAAGAAGC
CGGTGCATTGGTTGGCGTTGCGCCTATGAATCGCGAAAGTGGCCGCTATAAAGGGCTTCGAAAAATTCAAGGTGGGCGGCATCAAGTACGCACCGTTTTA
TACATGGCGATGATGTCAGCCATCCAATCAAACCCTGTTTTTAAATCAACTTATCAGCGATTAGTTGCTGCTGGGAAACCGAAAAAAGTAGCGCTTATTG
CGTGTGTCAGGAAGATGATTGTGATCTTAAATTCGATGCTCAGAGACGGAGTAATGTGGGAAGCGCCAAAAGTTTAAAATTAGCTATTGACGCCATAGTC
T

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
951 bp	316 aa	227	1177	+	No

ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MKTNTNQNINVGVDTGKFQLDIYIRPLDIYFTVKNDEKGIAEAIKKIKIHSPNRIIIEATGRLEMPFIIACAKANLPFVIANPIHIKRFAGAIGQRAKTD
KLDAQLIAHYGEAIKPTLSTLKPDIMQTMSDLVARRNQLLVMQTMEKNRLQILPKELISTIKPILTAFKNQIKKIENKILKLIESCPEYSAKNTILQSMK
GIGKIAAASIISNLPELGYMSNKEAGALVGVAPMNRESGRYKGLRKIQGGRHQVRTVLYMAMMSAIQSNPVFKSTYQRLVAAGKPKKVALIACVRKMIVI
LNSMLRDGVMWEAPKV

Blast result :

Comments

This 1201 bp element is a member of the IS110 family; the predicted transposase exhibits 76% amino acid identity with the MooV transposase of IS492. There are three complete copies in the P.tunicata genome and the element has the identical 5 bp DR sequence as seen for IS492 in P. atlantica.

References

1] Higgins, B., Popkowski, A., Caruana, P., and Karls, A. (2009) Submitted December 2008.
2] Thomas, T., Evans, F., Schleheck, D., Mai-Prochnow, A., Burke, C., Penesyan, A., Kalisay, D., Stelzer-Braid, S., Saunders, N., Johnson, J., Ferriera, S., Kjelleberg, S, and Egan, S. (2008) PloS ONE 3(9): e3252.