ISSse2
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009831 | ND | Shewanela sediminis | Shewanela sediminis |
DNA section
IS Length : 1234 bp
Ends
IR Length : 26/33
IRL : TGGTAAGCCCCTAGAACAGTAGACACTTCATAGAGTTATAATGGACCAAA
IRR : TGGTAAGCCCCTAGTTATCTAGACACTTTCTAGTTTCTCAAAATACTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAGCTGTTAC | AGG | GTTTGATGTT | 3 |
| GTTCAGGGTT | CTTTTGTAAG | 0 |
DNA sequence
TGGTAAGCCCCTAGAACAGTAGACACTTCATAGAGTTATAATGGACCAAAAATATGAGGTGTTACATGCGCGGAAAACGATATCCCGACGAGTTCAAAAT
CGAAGCAGTTAAACAGGTCACAGAGCGTGGTCACCAGATCGCCGATGTGGCAGAAAGATTAGGCGTCACTCCCAAAAGTTTGCACAACTGGATTAACAAG
TTTGATAAGCCAGAAAAGCAACACATCACCATTGATAATCAGCAAGACGAAATACGTAAACTGAAAGCGGAACTACGTCGGGTAACCGAAGAGAGAAATA
TCCTAAAGGAGGCCGCCGTGTACTTTGCAAGCGAGTCAAAGAAAAGTACACGTTCATAAAGTCCAGGCTCAAGCAATATCCTGTCAGAACCCTGTGTCGA
ATACTTGATGTTCACCACAGTGGCTTCTATGCGTGGCTAAAGGAGCCTTTGAGTAACCGAGAAGCTGAAGATAAAAGATTGCTAGGTAAGATTAAGCAAT
TTTGGCTGGAGAGTGGCTGTGTGTATGGTTATCGAAATATTACATTGGACCTCAAGGATGACGGTGAATCGGTTGGTAAAAATCGTGTGCATCGCATTAT
GAAAGCTGCTCAGATTAAGGCTGTACGAGGCTATAAGCGTAATCCAAGCTTTGGTGGTGGCGATGTTAGCCATACAGCACCAAACACGCTAAATCGTGGC
TTTGATGTCGCTAAGCCTAACAAAGTTTGGGTGACCGATTTTACTTATATCCGCACCCATGAAGGTTGGTTGTATCTAACTGTTGTGATCGACCTATTCT
CACGACAAGTTGTCGGTTGGACGATGAAAAGCACACCTAAGGCGGACTTGGTTATTGACGCGCTATTGATGGCGATATGGCGACGTTCTCCGACCGAAAA
GGTACTGATACATTCCGATCAAGGTGTGCAATATACCTGTTCAGACTGGCGTAGTTTTCTTAAAGAACACAACCTTGAAGCAAGTATGAGCCGCAGAGGA
AACTGTCATGATAACGCTGTTGCAGAGAGCTTTTTCTCACTCCTAAAGAAGGATAGAGTTAAGCGAAAAGTCTACAAAACCAGAGACGAAGCACGCTCTG
AAATATTTAACTATATAGAATATTTCTATAATCCAGTTCGACATCATGGTAGTAATAACGGACTGTCTCCGATGAAGTTCGAAAAGCAGTATTTTGAGAA
ACTAGAAAGTGTCTAGATAACTAGGGGCTTACCA
CGAAGCAGTTAAACAGGTCACAGAGCGTGGTCACCAGATCGCCGATGTGGCAGAAAGATTAGGCGTCACTCCCAAAAGTTTGCACAACTGGATTAACAAG
TTTGATAAGCCAGAAAAGCAACACATCACCATTGATAATCAGCAAGACGAAATACGTAAACTGAAAGCGGAACTACGTCGGGTAACCGAAGAGAGAAATA
TCCTAAAGGAGGCCGCCGTGTACTTTGCAAGCGAGTCAAAGAAAAGTACACGTTCATAAAGTCCAGGCTCAAGCAATATCCTGTCAGAACCCTGTGTCGA
ATACTTGATGTTCACCACAGTGGCTTCTATGCGTGGCTAAAGGAGCCTTTGAGTAACCGAGAAGCTGAAGATAAAAGATTGCTAGGTAAGATTAAGCAAT
TTTGGCTGGAGAGTGGCTGTGTGTATGGTTATCGAAATATTACATTGGACCTCAAGGATGACGGTGAATCGGTTGGTAAAAATCGTGTGCATCGCATTAT
GAAAGCTGCTCAGATTAAGGCTGTACGAGGCTATAAGCGTAATCCAAGCTTTGGTGGTGGCGATGTTAGCCATACAGCACCAAACACGCTAAATCGTGGC
TTTGATGTCGCTAAGCCTAACAAAGTTTGGGTGACCGATTTTACTTATATCCGCACCCATGAAGGTTGGTTGTATCTAACTGTTGTGATCGACCTATTCT
CACGACAAGTTGTCGGTTGGACGATGAAAAGCACACCTAAGGCGGACTTGGTTATTGACGCGCTATTGATGGCGATATGGCGACGTTCTCCGACCGAAAA
GGTACTGATACATTCCGATCAAGGTGTGCAATATACCTGTTCAGACTGGCGTAGTTTTCTTAAAGAACACAACCTTGAAGCAAGTATGAGCCGCAGAGGA
AACTGTCATGATAACGCTGTTGCAGAGAGCTTTTTCTCACTCCTAAAGAAGGATAGAGTTAAGCGAAAAGTCTACAAAACCAGAGACGAAGCACGCTCTG
AAATATTTAACTATATAGAATATTTCTATAATCCAGTTCGACATCATGGTAGTAATAACGGACTGTCTCCGATGAAGTTCGAAAAGCAGTATTTTGAGAA
ACTAGAAAGTGTCTAGATAACTAGGGGCTTACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 66 | 359 | + | No |
Description : First part of the transposase
ORF sequence :
MRGKRYPDEFKIEAVKQVTERGHQIADVAERLGVTPKSLHNWINKFDKPEKQHITIDNQQDEIRKLKAELRRVTEERNILKEAAVYFASESKKSTRS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 957 bp | 318 aa | 260 | 1216 | + | No |
Description : Second part of the transposase
ORF sequence :
TESGTTSGNRREKYPKGGRRVLCKRVKEKYTFIKSRLKQYPVRTLCRILDVHHSGFYAWLKEPLSNREAEDKRLLGKIKQFWLESGCVYGYRNITLDLKD
DGESVGKNRVHRIMKAAQIKAVRGYKRNPSFGGGDVSHTAPNTLNRGFDVAKPNKVWVTDFTYIRTHEGWLYLTVVIDLFSRQVVGWTMKSTPKADLVID
ALLMAIWRRSPTEKVLIHSDQGVQYTCSDWRSFLKEHNLEASMSRRGNCHDNAVAESFFSLLKKDRVKRKVYKTRDEARSEIFNYIEYFYNPVRHHGSNN
GLSPMKFEKQYFEKLESV
DGESVGKNRVHRIMKAAQIKAVRGYKRNPSFGGGDVSHTAPNTLNRGFDVAKPNKVWVTDFTYIRTHEGWLYLTVVIDLFSRQVVGWTMKSTPKADLVID
ALLMAIWRRSPTEKVLIHSDQGVQYTCSDWRSFLKEHNLEASMSRRGNCHDNAVAESFFSLLKKDRVKRKVYKTRDEARSEIFNYIEYFYNPVRHHGSNN
GLSPMKFEKQYFEKLESV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 66 | 1216 | + | Yes |
Chemistry : DDE
ORF sequence :
MRGKRYPDEFKIEAVKQVTERGHQIADVAERLGVTPKSLHNWINKFDKPEKQHITIDNQQDEIRKLKAELRRVTEERNILKEAAVYFASESKKKYTFIKS
RLKQYPVRTLCRILDVHHSGFYAWLKEPLSNREAEDKRLLGKIKQFWLESGCVYGYRNITLDLKDDGESVGKNRVHRIMKAAQIKAVRGYKRNPSFGGGD
VSHTAPNTLNRGFDVAKPNKVWVTDFTYIRTHEGWLYLTVVIDLFSRQVVGWTMKSTPKADLVIDALLMAIWRRSPTEKVLIHSDQGVQYTCSDWRSFLK
EHNLEASMSRRGNCHDNAVAESFFSLLKKDRVKRKVYKTRDEARSEIFNYIEYFYNPVRHHGSNNGLSPMKFEKQYFEKLESV
RLKQYPVRTLCRILDVHHSGFYAWLKEPLSNREAEDKRLLGKIKQFWLESGCVYGYRNITLDLKDDGESVGKNRVHRIMKAAQIKAVRGYKRNPSFGGGD
VSHTAPNTLNRGFDVAKPNKVWVTDFTYIRTHEGWLYLTVVIDLFSRQVVGWTMKSTPKADLVIDALLMAIWRRSPTEKVLIHSDQGVQYTCSDWRSFLK
EHNLEASMSRRGNCHDNAVAESFFSLLKKDRVKRKVYKTRDEARSEIFNYIEYFYNPVRHHGSNNGLSPMKFEKQYFEKLESV
Blast result :
Comments
There are 2 copies of ISSse2 encoded in the Shewanella sediminis genome. The ISSse2 transposase has 62% identity to ISGpr1_aa3. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Romine, M.F. (2008) Direct submission.